- Jing ZHANG;Wen XU;Dinghong WU;Dayuan ZHU;Chuanjian LU;Xiaohui QIU;
[Objective] To research on the chemical component changes of RADIX ASTRAGALI and RADIX NOTOGINSENG after compatibility by two different extracting methods. [Method] Changes of chemical components in compatibility extracting solution and single drug extracting solution were compared by HPLC. Combined with the molecular weight information provided by LC-MS, structures of the changed chemical components were deduced. [Result] Compared with the ethanol extracting method, water extracting-alcohol precipitating method had significantly higher contents of ginsenoside Rh1 and Rg2; and ginsenoside Rh4 and notoginsenoside T5 and Fe were detected only in the water extracting-alcohol precipitating solution; other 4 chemical component peaks were only detected in the ethanol extracting solution with low polarity, but they were not found in the water extracting-alcohol precipitating solution. [Conclusion] As for the compatibility extracting solution and single drug extracting solution, no new chemical components were detected in the extracting solution before or after compatibility, but the contents of some chemical components enhanced after compatibility. As for the different extracting methods, dissolution types and contents of chemical components changed significantly in compatibility extracting solution, especially the chemical components dissolution of RADIX NOTOGINSENG.
2013年01期 v.4 1-4+7页 [查看摘要][在线阅读][下载 203K] <正>The editorial board member of the Editorial Department of Medicinal Plant No. 568 of Binkang Road, Hangzhou City, China Tel: 0571-87774766 E-mail: wangrw@conbagroup.com Ruwei Wang, an eminent senior engineer and Ph.D. supervisor,
2013年01期 v.4 2页 [查看摘要][在线阅读][下载 249K] - Chunhong LI;Zhiqiang MEI;Jinping ZHANG;Ji TIAN;
[Objective] To research the optimal extraction technology of total flavonoids from young leaves of Toona sinensis (A.Juss) Roem. [Method] With the total flavonoids in young leaves of T. sinensis as the investigation index, extraction technology of total flavonoids was studied by orthogonal test. [Result] The optimal extraction conditions for total flavonoids from the young leaves of T. sinensis were 45 min ultrasonic extraction, 70% ethanol concentration, and 40 ml ethanol volume. Under this condition, the extraction rate of total flavonoids from the young leaves of T. sinensis reached 3.33%. [Conclusion] This method was reasonable and feasible, and could be used for the production.
2013年01期 v.4 5-7页 [查看摘要][在线阅读][下载 171K] - Xianshu WANG;Qin WANG;Jiannan SHI;
Using process simulation software Aspen Plus, distillation equilibrium citral separating process from Litsea cubeba oil was simulated by the Modified Group Contribution Method (UNIFAC-DMD). Based on the characteristics of L. cubeba oil components, vapor-liquid equilibrium between citral and other components was analyzed under the distillation pressure of 0.24 kPa.The two-stage distillation equilibrium separation route was established firstly; then sensitivity analysis of distillation temperature and pressure was carried out. The results showed that when the distillation pressure of the two stages was 0.24 kPa, distillation temperature of the first stage was 50 ℃, and that of the second stage was 58 ℃, the content of citral in distillation residue reached 93.35% and the total yield was 31.63%.
2013年01期 v.4 8-10+13页 [查看摘要][在线阅读][下载 283K] - Yanfeng TANG;Zhanzhong HOU;Zhibao WANG;Guoqiang FAN;
[Objective] To conduct map scanning of 41 types of rhubarb by near-infrared diffuse reflectance spectrometry, and to provide a new method for the pharmacognostic identification of rhubarb. [Method] Near-infrared spectra were compressed by wavelet transform technique. Training time was reduced; rhubarb samples in different production areas were identified by both near-infrared spectroscopy and clustering analysis method. [Result] After compression of wavelet transform by near-infrared spectroscopy, spectral variables reduced from 700 to 44. Wavelet compressed near-infrared spectra were inputted to clustering tree identification model. Based on clustering analysis, certified rhubarb was identified. And the recognition correct rate reached 82.93%. [Conclusion] Near-infrared diffuse reflectance spectrometry was a rapid and simple identification and analysis technology, and could be used for the quality control of rhubarb.
2013年01期 v.4 11-13页 [查看摘要][在线阅读][下载 187K] - Yundong SHI;
[Objective] To develop a method for the determination of Selenium in Chinese medicinal herb Erigeron breviscapus (Vant.) Hand.-Mazz.. [Method] Various test conditions and effect factors for determination of Selenium by flow injection-hydride generation-atomic absorption spectrometry (FI-HG-AAS) were discussed. [Result] The linear range for Selenium is 0-100 μg/L. The relative standard deviation is less than 2% and the recovery is 98%-102%. [Conclusion] This method is simple, rapid and has been applied to the determination of Selenium in Chinese medicinal herb E.breviscapus with satisfactory results.
2013年01期 v.4 14-15页 [查看摘要][在线阅读][下载 124K] - Kequn HE;Wenyun LU;Qiong YANG;Yuzhong ZHAO;
[Objective] To detect the content of total flavonoids in Hemiphragma heterophyllum Wall. [Method] Total flavonoids were extracted from H. heterophyllum by ultrasound assisted method. With rutin as the reference substance, relative percentage content was detected by UV-Vis spectrophotometry at 510 nm. [Result] Rutin reference substance showed good linear relationship within the range of 0 and 0.22 mg/mL (R2=0.999 7); and RSD value was 0.425%. The average value of total flavonoids in H. heterophyllum was 9.335%. [Conclusion] Detecting the total flavonoids content in H. heterophyllum by UV-Vis spectrophotometry was simple and reliable, which provided scientific references for the development of medicinal value of H. heterophyllum.
2013年01期 v.4 16-18页 [查看摘要][在线阅读][下载 199K] <正>CABI Database,one of the most authoritative and prestigious abstract data bases concerning agriculture and forestry,is compiled by Centre for Agricultural Bioscience International. This data base,since 1973,has embodied 5 million records,with an annual increase rate of 0. 18 million abstracts or so. The data,involving 70 kinds of languages,are from 14 000 periodicals,books,professional reports,proceedings and so on,which are published by more than 130 countries worldwide. It covers all the related disciplines
2013年01期 v.4 18页 [查看摘要][在线阅读][下载 110K] - Gang LIU;Yuchen LIU;Dewen QIU;Zhenyu WANG;Minghua QIU;
[Objective] To detect the polysaccharides content in Herminium bulleyi(Rolfe) Tang et Wang and its immunomodulatory effects. [Method] With glucose as the control substance, polysaccharides content in H. bulleyi was detected by phenol-sulphuric acid method. And the immunomodulatory effects of H. bulleyi polysaccharides on mice were researched. [Result] Content of H. bulleyi polysaccharides was 58.5%, which could significantly enhance the spleen weight of mice, promote the development of antibody, and enhance the humoral immunity of mice. [Conclusion] H. bulleyi polysaccharides had certain promotion effects on the specific immunity of organism.
2013年01期 v.4 19-21+24页 [查看摘要][在线阅读][下载 163K] - Xin CHEN;Zhongfa JIANG;Ning LI;Yuqin SHI;Benyan ZHANG;
[Objective] To explore the antagonistic effects of curcumin on formaldehyde-induced oxidative damage in cell. [Method] A549 cell were used as experimental materials, experiment was divided into normal control group, 0.1 mmol/L formaldehyde exposure group, and curcumine group (0.1 mmol/L formaldehyde + 2.5-20.0 mg/L curcumine). NOS, MDA, SOD and GSH-PX activities were detected in A549 cells. [Result] SOD, NOS and GSH-Px activities in A549 cells in formaldehyde exposure group were(21.79±1.13), (1.88±0.16) and (27.83±0.2)U/mg prot, respectively. Compared with control group, SOD, NOS and GSH-Px activities were significantly decreased (P<0.05), but MDA content increased significantly(P<0.05).Compared with the formaldehyde exposure group, GSH-Px activity in curcumin groups enhanced; and MDA content decreased (P<0.05). Compared with control group, differences in GSH-Px activity and MDA content in 40 mg/L curcumine group had no statistical significance (P>0.05). [Conclusion] Curcumin could enhance the antioxidant enzyme activity in A549 cells, showing certain dose-effect relationship.
2013年01期 v.4 22-24页 [查看摘要][在线阅读][下载 146K] - Along KANG;Yingshuang TANG;Jinqiang MA;
[Objective] To optimize the extraction technology of effective components from DAPHNES CORTEX by orthogonal test. [Method] The effects of ethanol concentration, ethanol dosage, refluxing time and refluxing times on the extraction of daphnetin, total coumarins and total flavonoids were investigated by orthogonal test with multi-index comprehensive evaluation. Data processing was carried out with the multiple guidelines grading method. [Result] The optimal extraction technology of effective components was that DAPHNES CORTEX was refluxed twice with 70% ethanol at the solid-liquid ratio of 1∶ 8 (g/mL), 2 h each time. [Conclusion] Because of well-designed design, this technology could extract effective components from DAPHNES CORTEX with reliable results and satisfied effects.
2013年01期 v.4 25-27+31页 [查看摘要][在线阅读][下载 187K] - Jianling JIN;Hui ZHANG;Bo LIU;Yupin CAI;Peiji GAO;
[Objective] To assay the mutagenicity of Enphorbia lunulata Bge decoction and to modify the Ames test for evaluating the mutagenicity of herbal medicine samples in vitro. [Method] The mutagenicity of E. lunulata decoction was assayed by standard Ames test. The teratogenicity was done by mammalian bone marrow chromosomal aberration test. In modified Ames test system, the influence of histidine in E.lunulata decoction was excluded by additional negative control, in which the test medium was supplied with histidine (histidine amount equaled to the histidine in different concentration of E.lunulata decoction). [Result] The mutagenicity of E.lunulata decoction was positive in standard Ames test. The teratogenicity of E.lunulata decoction was negative in mammalian bone marrow chromosomal aberration test. By the modified Ames test,the mutagenicity of E.lunulata decoction was negative. [Conclusion] The standard Ames test was not suitable for evaluating the mutagenicity of E.lunulata decoction, but the modified Ames test was. The mutagenicity in vitro and the teratogenicity in vivo of E.lunulata decoction were all negative.
2013年01期 v.4 28-31页 [查看摘要][在线阅读][下载 197K] -
<正>Chemical Abstracts ( CA) ,a reputable international search tool,which involves the widest disciplinary field,collects the most literature types,offers the most searching ways,and has the most prodigious volumes,is edited and published by CAS-Chemical Abstracts in the USA. CA has reported more than 10 000 kinds of technology journals,technology reports,meeting papers,academic
2013年01期 v.4 31页 [查看摘要][在线阅读][下载 101K] - Qiuxia MENG;Lizhen ZHANG;Wei NIU;Qiang JIN;Yu NIU;
[Objective] To prepare flavonoids and polyphenols with different polarity from roots and flowers of Caragana microphylla Lam., and to test their antimicrobial activities in vitro. [Method] Roots and flowers were subjected to crude extraction and liquid-liquid partitioning after ultrasound-assisted crude extraction. The antimicrobial activity of resultant extracts and fractions were determined, and the correlations between their antimicrobial activity and total flavonoid and total polyphenol contents were analyzed. [Result] The fractions and crude extract show inhibitory effect on Bacillus subtilis and Fusarium spp., but did not show substantial inhibitory effect against Staphylococcu saureus and Escheichia coli. Dichloromethane fraction of flower crude extract exhibited the highest inhibitory activity against B. subtilis and Fusarium spp., with titers of 176.78 IU/mg and 243.97 IU/mg, respectively. Correlation analyses suggested that flavonoids and polyphenols were compounds responsible for inhibitory effect of C. microphylla on B. subtilis and Fusarium spp. [Conclusion] Flavonoids and polyphenols from flowers and roots of C. microphylla may have various applications and great potential in development of plant-derived antimicrobial food additives, fresh fruit preservatives and medicines.
2013年01期 v.4 32-35+41页 [查看摘要][在线阅读][下载 226K] - Zhongqin LI;Ruizhang GUAN;Lihong WANG;Hongwei LIU;
[Objective] To study the in vitro inhibitory effects of traditional Chinese medicines on eel pathogens. [Method] Drug sensitivity tests of single and double-compound traditional Chinese medicines to 12 common eel pathogens in vitro were conducted by ultrafine powder agar dilution method. We studied on the inhibitory effects of single traditional Chinese medicines (RHIZOMA ET RADIX POLYGONI CUSPIDATI, PERICARPIUM GRANATI, RADIX ET RHIZOMA RHEI, RADIX SCUTELLARIAE, GALLA CHINENSIS, RHIZOMA COPTIDIS, RABDOSIA RUBESCENS, RADIX ANGELICAE PUBESCENTIS, RHIZOMA DRYOPTERIS CRASSIRHIZOMATIS, HERBA EQUISETI HIEMALIS) and the double-compound traditional Chinese medicines (GALLA CHINENSIS, RADIX ET RHIZOMA RHEI, PERICARPIUM GRANATI, RHIZOMA ET RADIX POLYGONI CUSPIDATI, RADIX SCUTELLARIAE and RHIZOMA COPTIDIS) on the 12 pathogenic strains isolated from diseased eels. [Result] The single traditional Chinese medicines in this research showed different degrees of inhibitory effects. Among them, GALLA CHINENSIS had the strongest antibacterial effects, followed with RADIX ET RHIZOMA RHEI and PERICARPIUM GRANATI. And HERBA EQUISETI HIEMALIS had the poorest antibacterial effects. 68.3% of the double-compound traditional Chinese medicines had synergistic effects (FIC<1); and medicines with significant synergistic effects accounted for 26.0%. Compound of RADIX ET RHIZOMA RHEI and PERICARPIUM GRANATI showed the optimal antibacterial effects. Compared with single traditional Chinese medicines, double-compound traditional Chinese medicines could enhance the inhibitory effects of the most pathogenic strains, and could greatly reduce the administration concentration of single medicine. [Conclusion] Single and double-compound traditional Chinese medicines all had certain in vitro inhibitory effects on eel pathogens.
2013年01期 v.4 36-41页 [查看摘要][在线阅读][下载 192K] <正>AGRIS (The Agricultural Sciences and Technology) is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO. It embodies continued publications,documents,corpuses,books,technology reports,patents,maps,meeting papers,and other literatures,which are collected by 135
2013年01期 v.4 41页 [查看摘要][在线阅读][下载 105K] - Yuanqing FU;Fengwei MO;Houzhi LIAO;
[Objective] To establish the method for content determination of kaempferol in Lysimachia fortunei Maxim. by HPLC. [Method] HPLC analysis for separation of kaempferol was performed on the Shimadzu VP-ODS C18 chromatographic column (150 mm×4.6 mm, 5 μm) with mobile phase of acetonitrile-0.2% phosphoric acid solution (V/V, 42∶ 58) at a flow rate of 1.0 mL/min. The detection wavelength was set at 370 nm. [Result] The linear range of kaempferol ranged from 0.206 2 to 1.031 0 μg/mL (r=0.999 8) with an average recovery rate of 98.67% (RSD=1.24%, n=6). [Conclusion] This method is accurate, sensitive and good in reproducibility. It can be applied for quality control of L. fortunei.
2013年01期 v.4 42-43+46页 [查看摘要][在线阅读][下载 155K] - Shijie LI;Yilong CHEN;Danyans ZHANG;
[Objective] To make the use of high performance liquid chromatographic method (HPLC) to determine the content of active ingredient adenosine in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from different production places in Guangdong Province. [Method] A HPLC method was established for determining the content of adenosine. To be specific, HPLC analysis was performed on a chromatographic column ZORBAXExtend-C18 (4.6 mm×250 mm, 5 μm) with methanol-water(V/V, 7∶93)as the mobile phase, detection wavelength at 260 nm, flow rate at 1.0 ml/min and column temperature at 30 ℃. [Result] Adenosine had good linear relationship between concentration and peak area from 4.65 to 232.6 ng/ml, the recovery rate was 100.3%, RSD=2.2%(n=6); the sample from Conghua in Guangdong Province had the highest content, while the two wild species had the lowest of that. [Conclusion] The method is simple, accurate, rapid and reproducible. It can complete the existing quality standard of RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE compound feed additives, and make the control of quality efficiently.
2013年01期 v.4 44-46页 [查看摘要][在线阅读][下载 176K] - Xinliang WANG;Jiliang WU;Min ZHENG;Ji ZHAO;
[Objective] To research on the inhibitory effects of tectorigenin from Pueraria Flos on the proliferation of human hepatoma cell line SMMC-7721. [Method] Total isoflavones in Pueraria Flos was obtained from the methanol extracts of wild Pueraria Flos by hydrochloric acid thermal hydrolysis. Total isoflavones were isolated by silica gel column so as to obtain tectorigenin. When the human hepatoma cell line SMMC-7721 was at logarithmic growth phase, different concentrations of tectorigenin were added for intervention. Changes of cell morphology were observed by inverted microscope. Inhibitory effects on hepatoma cell were analyzed by MTT; Annexin V-PI double staining was also adopted; effects of tectorigenin on cell early apoptosis were analyzed by flow cytometry. [Result] Tectorigenin significantly reduced the number of adherent hepatoma cells, turned the cancel cells to small and round ones. The inhibitory effects of tectorigenin enhanced sharply as the concentration increased within 1.0-8.0 μg/mL, showing significant dose-effect relationship. Under the concentrations of 5.00, 10.00 and 20.00 μg/mL, tectorigenin could significantly enhance the early apoptosis rate of hepatoma cells (P<0.01). [Conclusion] Tectorigenin showed significant inhibitory effects on the proliferation of human hepatoma cell line SMMC-7721. This mechanism might be related to the apoptosis promotion of cancel cell.
2013年01期 v.4 47-50页 [查看摘要][在线阅读][下载 359K] - Buming LIU;Kaijia HE;Wenjie LU;Sixiang LIU;Yan HUANG;Demei CHEN;
[Objective] To study chemical constituents of Mahonia duclouxiana Gagnep. [Method] The isolation and purification of the constituents were performed by chromatography and spectral analysis (TLC, IR, 1H NMR, 13C NMR, MS) from the ethanol extract.[Result] Six non-alkaloid monomeric compounds were extracted from M.duclouxiana stems. Those compounds were confirmed as 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl) propan-1-one (I), syringaresinol (II), syringaresinol-4-O-β-D-monoglucoside (III), daucosterol (IV), fumaric acid (V) and stearic acid (VI), respectively. [Conclusion] These six compounds are isolated and identified from this plant for the first time, and compound I is isolated and identified from this genus for the first time.
2013年01期 v.4 51-52页 [查看摘要][在线阅读][下载 132K] - Fangfang LIU;Qi XUE;Junzheng WANG;Zhiyang LIU;Yan LIU;
[Objective] To determine the content of quercetin-rhamnoside in different medicinal parts of Acanthopanax senticosus (Rupr. et Maxim.) Harms and compare them, so as to provide scientific basis for rational utilization and quality evaluation of A. senticosus. [Method] HPLC was used to determine the content of quercetin-rhamnoside in root, stem, leaf, flower and fruit. HPLC analysis was performed on Dalian Jiangshen Nucleosil C18 column (4.6 mm×150 mm, 5 μm) with mobile phase of methyl alcohol-water (V/V, 40∶ 60) and flow rate of 1.1 mL/min. The other conditions were: detection wavelength of 370 nm, column temperature of 30 ℃, sampling amount of 10 μL. [Result] Different medicinal parts of A. senticosus were arranged in order of quercetin-rhamnoside content: flower buds > leaves > fruits > stems > roots. The content of quercetin-rhamnoside was the highest in flower buds (4.43%), followed by leaves (0.61%), and the least in roots (0.01%). [Conclusion] This method is simple, quick and accurate, and can be used for analysis of quercetin-rhamnoside in different medicinal parts of A. senticosus.
2013年01期 v.4 53-54+57页 [查看摘要][在线阅读][下载 267K] - Ying HAO;Xingguo ZHANG;Yuwen CHEN;Xiaozhi LONG;Qian WANG;
[Objective] To study the relevance rules of flavonoid content of domestic high-quality Ophiopogon japonicus (Thunb) Ker-Gawl. tubers at different developmental stages and determine their quality characteristics, so as to provide a scientific basis for GAP quality control and utilization of O. japonicus. [Method] O.japonicus tubers from Hangzhou City (3-year-old) and Sichuan Province (1-year-old) were graded in accordance with the fresh tuber grading standards. After enzyme killing in microwave and drying at -50 ℃, the flavonoid content of O.japonicus tubers was determined by ultraviolet spectrophotometry. SPSS17.0 version statistical software was adopted to analyze the law of correlation between the average biomass and the flavonoids content of tubers at different developmental stages. [Result] The average flavonoid content of Hangzhou and Sichuan O. japonicus tubers at different developmental stages was 0.121%-0.192% and 0.135%-0.208%, respectively. With the increase of developmental stage, the accumulation of photosynthetic products was negatively related to the flavonoids content of O. japonicus tubers (P<0.001). The regression equation was written as y1=-0.047x+0.2 (r2=0.997) for Hangzhou O. japonicus and y2=-0.050x+0.217 (r2=0.992) for Sichuan O. japonicus. [Conclusion] The accumulation of photosynthetic products is closely related to the content of secondary metabolites flavonoids in O. japonicus tubers at different developmental stages. It is scientific to divide the domestic O. japonicus tubers by the traditional grade specifications.
2013年01期 v.4 55-57页 [查看摘要][在线阅读][下载 213K] - Keng WU;Qiong YOU;Ruina HUANG;Yanming TU;Jianguang LIANG;
[Objective] To explore protective effects of naringin on myocardial structure function with STZ-induced diabetic cardiomyopathy in rats via regulation of peroxisome proliferation factor receptor γ (PPARγ) and its possible mechanism. [Method] 80 male Wister experimental rats of Type 2 diabetic cardiomyopathy of 6-week old were randomly divided into five groups of 16 each, including the model control group, low-, medium-, high-dose naringin treatment groups, PPARγ agonist pioglitazone treatment group. 16 normal rats were used as the normal control. After oral administration of different doses of naringin (40, 20, 10 mg/kg·d), the expression of PPARγ was tested by immunohistochemistry and the expression of peripheral blood B brain natriuretic peptide (BNP) and cardiac troponin I (cTnI) were detected by quantitative immunofluorescence. The myocardial cell ultra-structure was observed under the transmission electron microscope. [Result] ① In the model control group, the enhanced PPARγ expression was prominently stained in endothelial cells and myocardial cell cytoplasm area. Compared with the normal control group, oral glucose tolerance test (OGTT) was positive, the level of heart/body weight, FFA, HOMA-iR, GSP and BNP increased, and the myocardial cell ultra-structure was injured in diabetes cardiomyopathy model control group. ② Compared with the model control group, the enhanced PPARγ expression were down-regulated, PPARγ positive vascular density weaken and the corresponding gray value increased (P<0.05), the injury in the myocardial cell ultra-structure and OGTT ameliorated, the level of heart/body weight, FFA, HOMA-iR, GSP and BNP decreased in the medium- or high-dose naringin and pioglitazone treatment groups. ③ Compared with the pioglitazone treatment group, the level of decrease was better in BNP but poor in HOMA-iR in the high-dose naringin treatment group (P<0.05). [Conclusion] Naringin could ease the injury in the myocardial cell ultra-structure. Its mechanisms may be associated to regulating myocardial PPARγ express mediated improvement of insulin resistance, blocking activation of FFA and inhibiting BNP.
2013年01期 v.4 58-61页 [查看摘要][在线阅读][下载 239K] - Xiaofeng ZHU;Tingchun WANG;Ronghua ZHANG;Panpan WANG;Li YANG;
[Objective] To observe the effects of total flavonoids in Drynaria fortunei (Kunze) J. Sm. (TFDF) on osteoblasts (OB) differentiation and the expression of p38 MAPK and BMP-2/Smads signaling in osteoblasts after treatment by Advanced Glycation End Products (AGEs), so as to explore the action mechanism for preventing and curing osteoporosis of TFDF. [Method] Primary osteoblasts of newborn Sprague-daweley rats were extracted and cultured by the double enzyme digestion method, and its biological characteristics were observed. pNPP, ELISA and Alizarin dyeing were respectively used to test ALP, Type I collagen (Col I), osteocalcin (BGP), BMP-2, and mineralization of osteoblasts after treatment by different concentration of AGEs and after treatment by different concentration of TFDF and AGEs (400 mg/L). Western-blot was used to detect p38 and Smad1/5/8 protein phosphorylation after treatment by TFDF and AGEs (400 mg/L). [Result] The levels of ALP, Type I collagen, osteocalcin and mineralization in osteoblasts treated by different concentrations of AGEs (200, 400 and 800 mg/L) were lower than those in the control group. TFDF could increase ALP, Type I collagen, osteocalcin, BMP-2 and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs (400 mg/L). TFDF (50 mg/L) can increase protein phosphorylation of p38 and Smad1/5/8 in osteoblasts after treatment by AGEs (400 mg/L). [Conclusion] AGEs can decrease differentiation and mineralization of osteoblasts. TFDF can increase differentiation and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs, which may be related to p38 and BMP-2/Smads signaling.
2013年01期 v.4 62-66页 [查看摘要][在线阅读][下载 304K] - Zhong LI;Yanling LU;Jing HUANG;Liangxing LI;Ganpeng LI;
[Objective] To study the flavonoids from Elsholtzia communis (Coll. et Hemsl.) Diels. [ Method] The compounds were isolated and purified by using a variety of separating materials combined with many kinds of separation means, their structures were identified by making use of 13C-NMR, 1H-NMR, MS, and other modern spectrum method. [ Result] Six flavonoids were isolated and identified from E. communis, including apigenin-7-O-β-D-glucoside (1), luteolin-7-O-β-D-glucoside (2), quercetin-7-O-β-D-glucoside (3), quercetin-4′-O-β-D-glucoside (4), apigenin-5-O-β-D-glucoside (5), and kaempferol-7-O-β-D-glucoside (6). [ Conclusion] All compounds are isolated from E. communis for the first time, of which compounds 3-6 are obtained from Elsholtzia for the first time.
2013年01期 v.4 67-68+71页 [查看摘要][在线阅读][下载 165K] - Songtao LI;Shulin ZHAI;Yong CHE;
[Objective] To optimize the extraction technology of tannins from Ziziphus jujuba roots, and to establish stable determination method of tannins content in Z. jujuba roots. [Method] Content of total tannins was detected by casein method. With tannins content as the evaluation index, the extraction time, solid-liquid ratio, extraction times, and acetone concentration were taken as the investigation factors. Extraction technology conditions were optimized by orthogonal design. [Result] Results showed that extraction times was the major influencing factor. The optimal extraction condition was as follows: 70% acetone concentration, 1∶20(W/V, g/mL)solid-liquid ratio, 30 min extraction time and 3 extraction times. Under this condition, the tannins content in Z. jujuba roots reached 6.82%. [Conclusion] The optimized extraction technology was reasonable, simple and feasible.
2013年01期 v.4 69-71页 [查看摘要][在线阅读][下载 173K] - Shuixian MA;Na ZHANG;Shuya ZHOU;Lijuan YANG;
[Objective] To study the preparation method and properties of inclusion complexes formed by alpinetin with cyclodextrins (α-CD, β-CD and γ-CD). [Method] Properties of inclusion complexes formed by alpinetin with cyclodextrins were studied by UV spectrum titration, XRD, DSC and so on. [Result] After the formation of inclusion complexes of alpinetin with cyclodextrins (α-CD, β-CD and γ-CD), the stability and solubility of alpinetin enhanced. [Conclusion] Alpinetin had application potential as medical molecular.
2013年01期 v.4 72-74页 [查看摘要][在线阅读][下载 270K] - Keke LI;Xiying LUAN;
[Objective] To research the immunoregulatory function of the ethanol extracts (BRE) from RHIZOMA BISTORTAE. [Method] In the mice in vitro test, phagocytic function of mice macrophages RAW264.7 on neutral red was detected by colorimetric analysis. In the mice in vivo test, viscera index was calculated according to the weight of spleen and thymus. T lymphocyte proliferation and NK cell activity were detected by MTT method. Level of serum hemolysin in mice was detected based on the immune of chicken erythrocyte. IL-2 content in serum was measured by ELISA method. [Result] BRE could significantly enhance the phagocytic function of macrophages RAW264.7 in mice, increase the spleen and thymus weight in mice, promote the proliferation of T lymphocyte, enhance the cytotoxicity of NK cell, and increase the levels of serum hemolysin and IL-2 in mice. [Conclusion] RHIZOMA BISTORTAE ethanol extracts (BRE) had certain immunoregulatory functions.
2013年01期 v.4 75-78+82页 [查看摘要][在线阅读][下载 188K] - Mengting GAO;Fangfang MA;Shaohui WU;Tingshun LUO;Chenggui ZHANG;Guangming LIU;
Icariin (ICA) is the main active ingredient of Epimedium brevicornum Maxim. that is a group of monomers of Chinese medicine plant having vast prospects of application. ICA has a variety of important pharmacological effects, such as protecting cardiovascular system, nourishing yin deficiency, tonifying yang, promoting multiplication and development of osteoblasts, regulating immune system, and anti-tumor, thereby having higher medicinal value and health care value. To fully develop this resource, the in-depth researches are done on pharmacological effects and clinical application of ICA, which have abroad developing prospects. A summary of ICA pharmacological research progress is made in this paper, aiming at providing basis for further development and utilization of ICA.
2013年01期 v.4 79-82页 [查看摘要][在线阅读][下载 190K] 下载本期数据