• Preliminarily Research on the Biological Characteristics of Silybum marianum Seeds

    SUN Jiang;LIU Xin-bo;

    [Objective]To research on the biological characteristics of Silybum marianum (L.) Gaertn. seeds. [Method] With the S. marianum seeds in three cultivation sites as the test materials, seed size and 1000-grain weight were measured; semi-thin section was adopted; and the germination situation of seeds under different temperatures were redearched. [Result] Seed was composed of embryo and scarfskin. Scarfskin consisted of five layers; the outwards four layers were peel, and the inwards was testa. Seed embryo developed well without endosperm, fat was the main storage material of cotyledon. The suitable temperature for seed germination was 25℃ and few seeds had dormancy phenomenon. [Conclusion] The basic biology data of S. marianum seeds were accumulated, which provided theoretical basis for the cultivation, classification and pharmacognostic identification of S. marianum seeds.

    2012年10期 v.3 1-3+7页 [查看摘要][在线阅读][下载 208K]
  • Introduction and Cultivation of Wild Glechoma longituba in Three Different Areas

    ZHANG Yan-ping;YANG Zhi-gang;CHEN Xian-guo;YI Xin-sheng;

    [Objective] To select the high-quality variety of Glechoma longituba (Nakai) Kupr., and to offer preparations for the large-scale cultivation and structure adjustment of the planting industrialization of Chinese medicinal materials. [Method] After three years' cultivation in Anshun Vocational and Technical College and in Shuangbao Town of Xixiu District in Anshun City, G. longituba from three different areas of Sichuan, Jiangxi and Anshun were transplanted in Drug Garden of Anshun Vocational and Technical College in 2008. Comparative tests on the cultivation were conducted, in order to investigate the plant growth, flowering, stems and leaves growth speeds, yield per plant, medicinal content and so on. The suitable variety of G. longituba for Guizhou Province was selected. [Result] Flowering habits of G. longituba in three areas were basically the same. The flowering periods were all in early spring, and lasted relatively long. The flowering seasons of G. longituba in Sichuan and Anshun were 4-7d longer than that in Jiangxi. The vegetative growth per plant and yields per plant of G. longituba growing in three different areas were in the order of Sichuan﹥Anshun﹥Jiangxi; yields and medicinal contents were in the order of Sichuan﹥Anshun﹥Jiangxi. [Conclusion] Due to its high yield and high quality, Sichuan G. longituba is the main extension variety in Guizhou Province at present. If the varieties having strong resistance to leaf spot could be selected from Anshun G. longituba, the yield could enhance greatly; and Anshun G. longituba could become an ideal variety for extension.

    2012年10期 v.3 4-7页 [查看摘要][在线阅读][下载 146K]
  • Distribution of Aquilaria sinensis (Thymelaeaceae)

    LI Lin-hai;SHOU Hai-yang;MA Qing-wen;

    Based on literature consultation, specimen information, and field investigation, distribution of Aquilaria sinensis (Lour.) Spreng. was analyzed. Results showed that A. sinensis was mainly distributed in Guangdong, Guangxi, Hainan, Yunnan, Hongkong, Macao and other areas of China. Besides, artificial cultivation of the trees was also found in Taiwan, Fujian, and arid-hot valleys of Jinshajiang River of Yunnan Province. At present, the reason for the endangered A. sinensis was the predatory cutting of wild trees, so that the seed trees became less and population regeneration was hard to be conducted smoothly. Therefore, resources status of A. sinensis should be studied, in order to provide scientific references for the sustainable development and utilization of A. sinensis.

    2012年10期 v.3 8-10+13页 [查看摘要][在线阅读][下载 157K]
  • Experimental Study on Using the Northern Climate Resource of the Yinshan Mountains to Plant Astragalus

    DU Wen-xu;HOU Hui-ping;JIANG Yan-feng;REN Xiao-meng;

    Using the principle of agroclimatic analogy, the feasibility of planting Astragalus in the northern area of Yinshan Mountains was acquired by the analysis of weather condition of Astragalus growing area. Combining introduction, comparison and field test, the planting effect of Astragalus in the northern area of Yinshan Mountains was analyzed. Experimental results showed that the northern area of Yinshan Mountains was suitable for the growth of Astragalus and its optimal sowing date was late autumn or early spring. To plant Astragalus in vast and hilly northern area of Yinshan Mountains could fix soil, protect slope, prevent wind erosion, prevent desertification, improve ecological environment, and make the most of climate resource. To accelerate the economic development of mountain area, we suggested expanding the planting scale of Astragalus in this district.

    2012年10期 v.3 11-13页 [查看摘要][在线阅读][下载 81K]
  • Resources Survey and Analysis of Wild FRUCTUS FORSYTHIAE

    WANG Jin-ming;FAN Sheng-ci;

    Based on relevant literatures, representative geographical distribution areas of wild FRUCTUS FORSYTHIAE were selected; filed investigation on wild FRUCTUS FORSYTHIAE was conducted. Results showed that there were many problems in the collection and processing, which greatly destroyed the wild FRUCTUS FORSYTHIAE resources. Therefore, protection and artificial cultivation of wild FRUCTUS FORSYTHIAE resources should be further strengthened, so as to realize the sustainable utilization of wild FRUCTUS FORSYTHIAE resources.

    2012年10期 v.3 14-16+19页 [查看摘要][在线阅读][下载 110K]
  • Effect of Winter LED Supplementary Illumination on Growing Development of Taxus chinensis var. mairei Cheng et L.K

    WANG Chao;HOU Yi-chong;WANG Wen;WANG Guo-fu;

    [Objective] To study the effect of winter LED supplementary illumination on growing development of Taxus chinensis var. mairei Cheng et L.K. [Method] The effect of winter LED supplementary illumination on growing development of T. chinensis in winter was studied by setting different intensities of supplementary illumination, including 1000, 3000, 5000, and 7000lx. [Result] The condition of 3000-7000lx LED supplementary illumination could promote the growth of T. chinensis while the condition of below 1000lx had little effects on its growth. And once the supplementary illumination was more than 5000lx, the effects got smaller. What's more, only about 126lx under LED plant growing lamp, the T. chinensis could bring the same net photosynthetic rate that under the condition of natural light (1257lx). [Conclusion] LED plant growth lamp is a kind of new light source that can bring higher net photosynthetic rate for plants at lower transpiration rate.

    2012年10期 v.3 17-19页 [查看摘要][在线阅读][下载 126K]
  • Key Technologies of Chinese Ginseng (Panax ginseng C.A.Meyer) History and Different Planting Models

    LV Long-shi;PENG Hao;

    In this paper, we studied the medicinal history and planting history of Chinese ginseng (Panax ginseng C.A.Meyer) and discussed the key technologies of its different planting models according to relevant data in combination with previous survey activities and scientific research achievements. In addition, this paper points out the direction of ginseng planting, one is to develop woodland ginseng planting, the other is to develop non-woodland ginseng planting.

    2012年10期 v.3 20-24页 [查看摘要][在线阅读][下载 126K]
  • Effects of Instant Asparagus Powder on Immune Function of Mice

    LI Han-chen;ZHAO Xi-yan;LIU Shao-jun;PENG You-shun;

    [Objective] To study the effects of instant asparagus powder (IAP) on immune function of mice. [Method] According to the method recorded in the Technical Standards for Testing & Assessment of Health Food (2003), the mice were given instant asparagus powder (referred to as the extract of Asparagus officinalis) for successive 30 days by intragastric administration. [Result] Instant asparagus powder could improve evidently functions of cellular immunity, humoral immunity, mononuclear macrophage and activity of NK on mice. But apparent effect to weights of spleen, thymus and body were not seen in the trials. [Conclusion] Instant asparagus powder can improve the immune function of mice.

    2012年10期 v.3 25-26+29页 [查看摘要][在线阅读][下载 97K]
  • Research Progress of the Propagation of Yinhua

    CHEN Shao-rong;NIE Chun;CHENG Ruo-min;XIN Ning;

    Seed propagation, cutting propagation, layering propagation, division propagation, grafting propagation, grafting propagation and tissue culture of Yinhua were reviewed in recent 10 years, so as to provide references for the standardized propagation technologies of Yinhua.

    2012年10期 v.3 27-29页 [查看摘要][在线阅读][下载 99K]
  • Effects of NaCl Stress on the Scavenging Activity of Limonium sinense Kuntze to Reactive Oxygen Species

    ZHANG Xia;YIN Hai-bo;CHEN Shi-hua;LI Li-xia;ZHAO Ji-qiang;GUO Shan-li;

    [Objective] To discuss the effects of NaCl stress on the scavenging activity of Limonium sinense Kuntze to reactive oxygen species (ROS). [Method] After L. sinense seedlings were treated by 500mmol/L NaCl, the osmotic potential of leaves, content of MDA, and activities of catalyses (CAT), peroxidase (POD), superoxside dismutase (SOD) were investigated. [Result] The content of MDA maintained a relatively low level, while it reached the maximum value after treatment for 5 days. The antioxidase activities increased first and then decreased, and reduced to the minimum level after treatment for 5 days, which was consistent with the results of MDA content. [Conclusion] Depending on antioxidant enzyme system, L. sinense could scavenge reactive oxygen species, reduce oxidative damage and maintain growth during a short period under NaCl stress.

    2012年10期 v.3 30-32+36页 [查看摘要][在线阅读][下载 423K]
  • Morphological and Microscopic Identification of Zhuang Folk Medicine Shishanglian

    TAN Yuan-feng;YUAN Xian-ling;QIU Fen;MO Fang;HUANG Mao-chun;

    [Objective] To conduct the morphological and microscopic identification of Zhuang Folk Medicine Shishanglian. [Method] Microscopic identification of Zhuang Folk Medicine Shishanglian was conducted according to the relevant literatures and crude drug knowledge. [Result] There are about 18 vascular bundles in the rhizome of Shishanglian, which arranged alternately in a circle with unequal spacing. Calcium oxalate sand crystal exists in rhizomes, petioles, leaves and powders of Shishanglian. Epidermal cells in transverse section of leaves are relatively big; and the lower epidermal cells are greater than the upper epidermal cells. Stomas are arranged in a circle. Subsidiary cells are arranged in 3 circles, with 3 cells in each circle. [Conclusion] The morphological and microscopic identification of Zhuang Folk Medicine Shishanglian were studied, aiming at providing references for the quality control of Zhuang Folk Medicine Shishanglian.

    2012年10期 v.3 33-36页 [查看摘要][在线阅读][下载 703K]
  • Protective Effects of Extracts from E. ulmiodes Leaves on Liver and Quadriceps Femoris Tissue of Mice at Exhaustive Exercise and Recovery

    LIU Jing;

    [Objective] To study the effects of extracts from Eucommia ulmiodes Olirer leaves (EEUOL) on the levels of SOD, POD and MDA in liver and quadriceps femoris tissue and the levels of AST, ALT and AKP in serum of mice at exhaustive exercise and recovery. [Method] A total of 72 ICR mice were randomly divided into 6 groups of 12 each: quiet control group (A), quiet EEUOL group (B), exhaustive exercise control group (C), exhaustive exercise EEUOL group (D), exhaustive exercise recovery control group (E) and exhaustive exercise recovery EEUOL group (F). Mice in drug groups were given EEUOL at the dosage of 1.5g/kg per day for successive 30 days by intragastric administration, while those in control groups were given distilled water. The exhaustive exercise time of mice in exercise groups was recorded when they swam in the water until exhaustion. After exercise, the blood was immediately sampled from eyeball of mice in groups C and D, the liver and quadriceps femoris tissue were taken from their bodies for later use. Because of mice in groups A and B were not given exercise treatment, the blood was directly sampled from eyeball, and the liver and quadriceps femoris tissue were taken from their bodies. As to groups E and F, the blood was sampled from eyeball after 24h recovery, and the liver and quadriceps femoris tissue were taken from their bodies. Spectrophotography was used to measure the contents of MDA, the activity of SOD and POD in liver and quadriceps femoris tissue, and the activity of AST, ALT and AKP in serum of mice at exhaustive exercise and recovery. [Result] EEUOL could increase the activities of SOD and POD in liver and quadriceps femoris tissue of mice at exhaustion and reduce the levels of MDA in liver and quadriceps femoris tissue of mice at exhaustive exercise and recovery. EEUOL could also reduce the activities of AST, ALT and AKP in serum of mice at exhaustive exercise and recovery. [Conclusion] Extracts from Eucommia ulmiodes Olirer leaves have a protective effect on liver and quadriceps femoris tissue of mice at exhaustive exercise, and have an accelerated effect on mice recovery.

    2012年10期 v.3 37-38+42页 [查看摘要][在线阅读][下载 100K]
  • Scavenging Effects of Purified Burdock Polysaccharides on DPPH· Free Radical by EPR

    HU Jian;LI Zhe-jin;KONG Fan-wei;GU Lin;

    [Objective] To detect the clearance rate of DPPH· free radical by purified Burdock polysaccharides. [Method] Scavenging effect of purified Burdock polysaccharide on DPPH· free radical were detected by Electron Paramagnetic Resonance (EPR). [Result] When the mass ratio of purified Burdock polysaccharides to DPPH· free radical reached 10.0, DPPH· free radical could not be detected after 5min; when the mass ratio was 4.67, a total of 50% DPPH· free radical could be removed. [Conclusion] Purified Burdock polysaccharide had certain scavenging effects on DPPH· free radical.

    2012年10期 v.3 39-42页 [查看摘要][在线阅读][下载 304K]
  • Effects of Essential Oil from Chimonanthus praecox on Rat Adjuvant Arthritis and Their Mechanisms

    QIAN Hai-bing1;LUO Kui;LONG Qing-long;JIN Feng-yun;

    [Objective] To study the effects of essential oil from Chimonanthus praecox on rat adjuvant arthritis and their mechanisms. [Method] Rats were divided randomly into normal control group, model control group, positive drug group and high-, middle- and low-dosage groups of essential oil from C. praecox (10.0, 5.0 and 2.5g/kg crude drug). Adjuvant arthritis (AA) rat model was established by subcutaneous injection of Freund's adjuvant (FCA) except the normal group. Drugs were given by oral gavage for 21d continuously from the modeling day. The anti-arthritic effects and mechanism of essential oil from C. praecox in Guizhou against RA were studied through observing the paw swelling, viscera index pathological changes and levels of interleukin 1β(IL-1β) and tumor necrosis factor α(TNF-α) after medication. [Result] The essential oil from C. praecox could effectively inhibit the rat paw edema, compared with model group. But it had no significant effects on the immune organs. The contents of IL-1β and TNF-α in the serum of AA rats decreased significantly compared with model group. [Conclusion] The essential oil from C. praecox had certain therapeutic effects on rat adjuvant arthritis, and its mechanism might be related to the reduction of cytokine generation.

    2012年10期 v.3 43-45+48页 [查看摘要][在线阅读][下载 228K]
  • Effects of Toona sinensis Protein on the Antioxidant Capacity and Nonspecific Immunity of Mice

    ZHANG Lin-su;XIA Ya-lan;PENG Ze-ping;

    [Objective] To study the effects of Toona sinensis protein on the antioxidant capacity and nonspecific immunity of mice. [Method] Crude protein was extracted from T. sinensis by ammonium sulphate precipitation, freeze drying, and dialysis. Mice were given T. sinensis protein by intraperitoneal injection for 9d in high-, middle- and low-dosages. Serums of half of mice were selected and their SOD and GSH-Px were detected by kits. Liver, spleen, and thymus were anatomized. MDA content and spleen and thymus indices were detected. The rest half mice were given starch broth at 10d by intraperitoneal injection. Then, peritoneal fluid was taken out and the phagocytic rate of macrophage was observed. [Result] Content of T. sinensis crude protein was 84.5% and the extraction rate was 5.3%. T. sinensis protein could significantly enhance the activity of SOD in mice serum (P<0.05,high dosage P<0.01). High-dosage T. sinensis protein could enhance the content of GSH-Px (P<0.05), spleen index (P<0.05,high dosage P<0.01), and phagocytic rate of macrophage (P<0.05). [Conclusion] T. sinensis protein had certain in vivo antioxidant capacity, and could promote the nonspecific immunity of mice.

    2012年10期 v.3 46-48页 [查看摘要][在线阅读][下载 146K]
  • Infrared and Thermal Analysis Identification of Cirsium shansiense Petrak and Potentilla discolor Bunge

    TAO Ming;LUO Qian;HUANG Yan;

    [Objective] To establish identification methods for Cirsium shansiense Petrak and Potentilla discolor Bunge. [Method] IR fingerprint and TG-DTG curve analyses of C. shansiense and P. discolor were conducted by infrared spectrometer and TG-DTG. [Result] IR fingerprint and TG-DTG curve of C. shansiense and P. discolor had different characteristics. [Conclusion] The IR fingerprint and TG-DTG curve could be used for the identification of C. shansiense and P. discolor.

    2012年10期 v.3 49-50+53页 [查看摘要][在线阅读][下载 176K]
  • Protective Effects of APS-A1 on Blood Vessel Endothelium Function Induced by Isofenphos-methyl

    YIN Ya-ling;ZHANG Xiao-yi;MA Li-juan;ZHAO Fan-rong;

    [Objective] To observe the protective effects of APS-A1 on blood vessel endothelium function induced by isofenphos-methyl. [Method]The ex vivo aorta pectoralis vascular ring (EVAPVR) of rats and cultured human umbilical vein endothelial cells (HUVEC) were exposed to medium contained isofenphos-methyl oxon (7μmol/L), APS-A1 for difference concentration were used to inhibit the damage effect. The endothelial-dependent relaxation reaction (EDRR), endothelial cell monolayer permeability (ECMP), and biochemical index were measured. [Result] APS-A1 dose dependently (0.1, 1, 10 mg/ml) reduced the inhibition of ACh-induced EDRR and the increased ECMP induced by isofenphos-methyl, simultaneously APS-A1(10mg/ml) also protected the SOD activity, inhibited the increase of the MDA content and reduction of NO content induced by isofenphos-methyl in medium of cultured HUVEC. [Conclusion] APS-A1 can protect the blood vessel endothelium function from been impaired by isofenphos-methyl, and the potential mechanism is possibly concerned with the antioxidation of APS-A1.

    2012年10期 v.3 51-53页 [查看摘要][在线阅读][下载 102K]
  • Study on HPLC Fingerprint of Active Ingredients in FRUCTUS MOMORDICAE

    ZHENG Qi;

    [Objective] To establish a representative HPLC fingerprint of FRUCTUS MOMORDICAE for quality control of its drugs. [Method] Under certain chromatographic conditions, the fingerprint of FRUCTUS MOMORDICAE was studied using high performance liquid chromatography (HPLC). Next, the similarity of chromatographic fingerprint was analyzed using evaluation system software. [Result] This fingerprint detection method established by HPLC was stable, precise and reproducible. [Conclusion] The fingerprint detection method of FRUCTUS MOMORDICAE can be used for its identification and quality control of medicines.

    2012年10期 v.3 54-55+60页 [查看摘要][在线阅读][下载 148K]
  • Effects of Forsythiaside on the Expression of IFN-α and Mx1

    MA Yuan-yuan;ZHANG Zhong-wen;LI Hua-wei;ZHANG Yu;WU Guo-juan;

    [Objective] To discuss the effects of forsythiaside on the expression of IFN-α and Mx1 based on mice model infected by PCV2, and to evaluate the antiviral effects of forsythiaside. [Method] Mice model infected by PCV2 was established; and high-, middle- and low-dosage groups (2, 4, 10mg/ml forsythiaside) were set up. After administration for 12, 24 and 36h, total RNA and total protein were extracted from mice lung tissues. Real-time FQ-PCR was used for the detection of IFN-α and Mx1 mRNA contents. Western blotting was used to detect the expression level of Mx1 protein. [Result] In blank control group, there was no expression of Mx1 or IFN-α in mice lung tissue, but there was little expression in virus control group. With the increase of forsythiaside concentration, IFN-α and Mx1 levels gradually enhanced; and the two showed parallel correlation in expression level. IFN-α and Mx1 expression levels were the maximum in high-dosage group (P<0.001). With the extension of drug treating time, expression level reduced, reached the maximum at 12h (P<0.001), and then gradually reduced. [Conclusion] Kunming mice were suitable for the establishment of PCV2 infected model. Forsythiaside could significantly enhance the expression level of IFN-α and Mx1, and showed significant antiviral effects after administration for 12h. Mx1 protein had close correlation with virus infection and could be used for the early diagnosis of virus infection of animals.

    2012年10期 v.3 56-60页 [查看摘要][在线阅读][下载 235K]
  • Optimization of the Extraction Technology of Total Alkaloids from Aconitum gymnandrum Maxim

    XIANG Gang;TAN Chuan-jiang;

    [Objective] To optimize the extraction technology of total alkaloids from Aconitum gymnandrum Maxim. [Method] With the extraction rate and aconitine content as the indices, effects of extraction time, extraction temperature, and soaking time on the extraction rate of total alkaloids were studied by L9(34) orthogonal test. [Result] Factors affecting the extraction rate of total alkaloids from A. gymnandrum were in the order of extraction time (C) > soaking time (B) > extraction temperature (A). The optimal extraction condition was A3B3C2, which was 80℃ extraction temperature, 36h soaking time, and 30min extraction time. Under this condition, extraction rate of aconitine was the highest. [Conclusion] This method was rapid, stable and could reduce the loss of the effective components of aconitine during operation. The extraction technology of total alkaloids from A. gymnandrum was optimized, which provided basis for the further research on the total alkaloids in A. gymnandrum.

    2012年10期 v.3 61-63+69页 [查看摘要][在线阅读][下载 178K]
  • Extraction Technology of Flavonoids from Ginkgo biloba L. Shells

    LIANG Xiao-feng;

    [Objective] To study the extraction technology of flavonoids from Ginkgo biloba L. shells. [Method] With ethanol solution as the extraction solvent, flavonoids were extracted from G. biloba shells. Effects of ethanol concentration, solid-liquid ratio, extraction time and extraction temperature on the extraction rate of flavonoids were studied by single factor test. The optimal conditions of traditional ethanol extraction and microwave-assisted extraction were researched by orthogonal test. [Result] The optimal condition of traditional ethanol extraction was as follows: 60℃ extraction temperature, 75% ethanol concentration, 1∶45 solid-liquid ratio (W/V, g/ml), and 2h extraction time. Under this condition, the extraction rate of flavonoids reached 0.503 4%. The optimal condition of microwave-assisted extraction was as follows: 60℃ microwave temperature, 45% ethanol concentration, 1:30 solid-liquid ratio, and 480s microwave time. Under this condition, the extraction rate of flavonoids reached 0.815%. [Conclusion] Microwave-assisted extraction was rapid, simple and high-efficient. As a new extraction technology, microwave-assisted extraction was superior to traditional ethanol extraction in certain extent.

    2012年10期 v.3 64-69页 [查看摘要][在线阅读][下载 397K]
  • Optimization of the Extraction Technology of Camptothecin and 10-hydrxycamptothecin by Orthogonal Design

    ZHAO Jin-liang;TANG Xiao-dan;YUN Da;HANG Hai-yang;

    [Objective] To select the optimal ultrasonic extraction technology of camptothecin (CPT) and 10-hydrxycamptothecin (HCPT) from Camptotheca acuminate Decne fruits. [Method] Contents of camptothecin and 10-hydrxycamptothecin were detected by high performance liquid chromatography. Based on single factor test, L9(34) orthogonal test was conducted to determine the optimal extraction condition. [Result] The optimal extraction condition of camptothecin was as follows: 70% methanol, 1:30 (g/ml) solid-liquid ratio, 60℃ extraction temperature, and 25min extraction time. The optimal extraction condition of 10-hydrxycamptothecin was as follows: 60% methanol, 1:30 (g/ml) solid-liquid ratio, 60℃ extraction temperature, and 20min extraction time. Verification test showed that the average extraction rates of camptothecin and 10-hydrxycamptothecin were 0.719‰ and 0.238‰, with RSD being 1.99% and 1.79%, respectively. [Conclusion] This method was stable and feasible. And the optimal extraction technologies of camptothecin and 10-hydrxycamptothecin from C. acuminate fruits were obtained.

    2012年10期 v.3 70-71+74页 [查看摘要][在线阅读][下载 95K]
  • Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L. Stems by Orthogonal Design

    GUO Li-qiang;HUANG Li-de;ZHANG Zhao-ping;PAN Ting-qi;WEN Quan-tai;YAN Zu-di;HUANG Suo-yi;

    [Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems. [Method] The cellulase extraction process of polysaccharides from C. lachrymal-jobi stems was optimized by means of orthogonal test design. And the content of polysaccharides in extracts was determined by spectrophotometry. [Result] The wavelength of the maximum absorption was 490nm. Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100mg/ml (R=0.9993). The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5, amount of enzyme was 6.0ml, the reaction temperature was 40℃, and enzymatic hydrolysis time was 150min. Under these conditions, the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%. [Conclusion] The enzyme extracting procedure is reasonable, economical and feasible.

    2012年10期 v.3 72-74页 [查看摘要][在线阅读][下载 125K]
  • Determination of Aloin Content in Aloe with HPLC and Analysis of Aloe Chromatogram

    YANG Su-ping;TIAN Jia-ming;SUN Dong-xiao;ZHEN Pan;

    [Objective] To establish a HPLC method for determining the content of aloin in 5 species of Aloe, and analyze their chromatograms, so as to distinguish them from each other. [Method] The content of aloin in sample solutions of Aloe species prepared using ultrasonic wave method was measured by HPLC. And, the chromatograms of Aloe were obtained. [Result] The linear regression equation of aloin was Y=13.1684X-2.8554, R=0.9998, showing good linearity between concentration and peak area in the range of 2.92 to 46.67μg/ml. The average recovery rate was 108.0%. The content of aloin was 614.3μg/g for A. arborescens, 212.5μg/g for A. delaetii, 135.3μg/g for A. vera L., 72.92μg/g for A. vera L. var. chinesis (Haw.) Berg, and 2.62μg/g for A. nobilis. Double peaks occurred at the retention time of 3 to 5min in the HPLC chromatogram of A. vera L., the peak height of which was slightly higher on the left, but this difference was not significant. Its double peaks were basically separated at the baseline. From then on, no obvious chromatographic peaks appeared on the chromatogram. In addition, the chromatogram of A. vera L. was similar to that of A. arborescens and very different from that of other three species. [Conclusion] There is a clear difference in aloin level among 5 species of Aloe. Of these, the content of aloin in A. arborescens is the highest. The determination method is stable, accurate and quick. Five species of Aloe are distinguished from each other by means of chromatogram analysis.

    2012年10期 v.3 75-77+80页 [查看摘要][在线阅读][下载 227K]
  • Comparison of the Effective Components Extraction from Compound Chinese Herbal Medicine by Two Different Methods

    XIE Shu-ling;ZHANG Li;

    [Objective] To compare the effective components extraction from compound Chinese herbal medicine by microwave-assisted extraction and ethanol reflux extraction. [Method] Effective components were extracted from compound Chinese herbal medicine by ethanol reflux extraction and microwave-assisted extraction. At the same time, baicalin and puerarin contents were detected by UV-Vis spectrophotometry. With the extraction rates of baicalin and puerarin as the indices, extraction efficiency of the two extraction methods were compared. And the effects of extraction time on extraction rate by microwave-assisted extraction were studied. [Result] Ethanol with different concentrations was used for reflux extraction. Extraction rates of baicalin and puerarin firstly enhanced and then reduced as the ethanol concentration increased. When using microwave-assisted extraction, extraction rates of baicalin and puerarin were the highest by 60% ethanol. When the ethanol concentration was 60%, extraction rates of baicalin and puerarin by microwave-assisted extraction were significantly higher than those by ethanol reflux extraction. The optimal extraction time of microwave-assisted extraction was 15min. Under this condition, the extraction rates of baicalin and puerarin were the highest, which were 0.3155 and 0.5025mg/g, respectively. [Conclusion] Microwave-assisted ethanol reflux extraction could significantly enhance the extraction rates of baicalin and puerarin.

    2012年10期 v.3 78-80页 [查看摘要][在线阅读][下载 95K]
  • Determination of Trace Elements in Potentilla discolor Bge

    LUO Qian;HUANG Yan;ZHU Jing-ping;TAO Ming;

    [Objective] To detect the contents of trace elements in Potentilla discolor Bge of traditional Yi medicine, so as to provide references for the development and application of P. discolor. [Method] P. discolor sample was digested by microwave with concentrated nitric acid. Then, Fe, Cu, Zn, Mn, Ca and Mg contents in P. discolor sample were detected by FAAS. [Result] P. discolor was rich in various essential trace elements for human body. This method had good linear relationship (R=0.9957-0.9982); the average recovery rate was 96.4%-107% (n=8); and RSD was 1.9%-5.5%(n=8). [Conclusion] This method was simple and accurate and could be used for the continuous detection of many elements in P. discolor.

    2012年10期 v.3 81-82+86页 [查看摘要][在线阅读][下载 97K]
  • Separation of β-elemene from Eupatorium adenophorum by Macroporous Resin Column Chromatography

    WEI Hui-ping;ZHAO Mu;LI Yong;LIU Wen-quan;YAN Yan;ZHENG Yi;

    [Objective] To extract β-elemene from worst weed Eupatorium adenophorum Spreng, identify its structure and determine its content. [Method] Having obtained volatile oil from E. adenophorum by "water+glycol" distillation, β-elemene was separated by column chromatography with slica, silver nitrate modified slica, D101 and HP20 macroporous resin respectively. The pure product of β-elemene was prepared by HPLC, and its molecular structure was validated by UV, IR, 1H NMR, 13C NMR and HRMS. [Result] The purity and yield of product was up to 63.1% and 75.6% respectively if using HP20 column chromatography, the optimal one for separation of β-elemene from E. adenophorum. Through three steps of "water+glycol" distillation, HP20 column chromatography and HPLC preparation, the β-elemene product with 98.6% purity and 36.5% yield could be obtained from E. adenophorum. [Conclusion] HP20 column chromatography is the most efficient way to separate β-elemene from E. adenophorum, which has the advantages of low cost and easy operation.

    2012年10期 v.3 83-86页 [查看摘要][在线阅读][下载 239K]
  • Determination of Fe, Ca, Mn, Cr, Ni and Cu in Allium macrostemon by AAS

    REN Yu-lan;ZHENG Wei;YING Zhi-wei;JIN Chao;DI Ji-peng;

    [Objective] To measure the content of iron, calcium, manganese, chromium, nickel and copper in Allium macrostemon. [Method] An investigation about the effects of wet digestion and dry ashing method on the determination results were performed on raw material A.macrostemon, sampled from Xishan Mountain in Mudanjiang City. Atomic absorption spectrometry (AAS) was adopted to determine the content of iron (Fe), calcium (Ca), manganese (Mn), chromium (Cr), nickel (Ni) and copper (Cu) in A. macrostemon. Precision test and recovery test were made. [Result] A. macrostemon contained many kinds of nutrient elements that were essential to human body, among which the content of calcium was the highest at 1.2407mg/g, of iron was the middle, of manganese and chromium was the lowest. Besides, nickel and copper could not be detected. [Conclusion] This method has the advantages of time-saving, labor-saving, high sensitivity, fast speed of analysis, and high accuracy, so that it is fit for the analysis of constant and trace elements in A.macrostemon.

    2012年10期 v.3 87-88+91页 [查看摘要][在线阅读][下载 160K]
  • Determination of the Diosgenin Content of Dioscorea opposita Thunb. Tubers and Bulbils

    TENG Jing-tong;GAO Xiang;XUE Jian-ping;LI Zi-wei;ZHANG Ai-min;SHENG Wei;

    [Objective] To determine the diosgenin content of Dioscorea opposita Thunb. tubers and bulbils. [Method] With 5% vanillin-glacial acetic acid-perchloric acid as color developing agent and oleanolic acid as the control substance, the content of diosgenin in D. opposita tubers and bulbils was measured using an ultraviolet visible spectrophotometer at the detection wavelength of 547nm. [Result] The content of diosgenin in D. opposita tubers and bulbils was 0.0164% and 0.0213%, respectively. The average recovery of diosgenin was 98.46% with relative standard deviations (RSD) of 1.36%. Besides, the content of diosgenin in bulbil was slightly higher than that in D. opposita tubers. [Conclusion] The method is simple, easy, accurate, and suitable for content determination of diosgenin in D. opposita tubers and bulbils.

    2012年10期 v.3 89-91页 [查看摘要][在线阅读][下载 137K]
  • Tissue Culture of the Shoot Tips of Bozhou Paeonia lactiflora Pall

    MA Zong-xin;MA Tong-fu;MENG Dong-qing;

    [Objective] To study the effects of culture conditions on the tissue culture of shoot tips of Bozhou Paeonia lactiflora Pall. [Method] Culture mediums with different concentration combinations of 6-BA, NAA and IBA were prepared. Based on the cultivation results, the optimal formula was selected. [Result] After shoot tips of Bozhou P. lactiflora were cultivated on 1/2 MS+ 1.0mg/L 6-BA for 39d, buds and multiple shoots were differentiated. Multiple shoots showed the rapidest proliferation on 1/2 MS+ 1.0mg/L6-BA, and the optimal rooting results on 1/2 MS+0.1mg/L IBA. [Conclusion] Concentration of plant growth regulator directly affected the tissue culture of shoot tips of Bozhou P. lactiflora. And shoot tips could directly grow into seedlings under appropriate culture medium.

    2012年10期 v.3 92-94+99页 [查看摘要][在线阅读][下载 159K]
  • Comparison of Polysaccharides Content and Growth Status of Nine Ganoderma Strains Cultivated on JUNCAO

    WANG Kai;LIN Zhan-xi;CAO Jian-hong;

    [Objective] To compare the polysaccharides content and growth status of nine Ganoderma strains cultivated on JUNCAO, and to optimize the optimal strain for cultivation. [Method] Nine strains were selected for JUNCAO cultivation, which were G10004, Taishan G10014, Xinzhou G10002, Sanmingsuo G10001, Korean G10006, G10022, G. lucidum G10008, G10021 and Ga0801. The polysaccharides content and growth status of Ganoderma strains were compared. [Result] Strain G10021 was in good growth status, and had relatively high polysaccharides content. Strains Ga0801 and Taishan G10014 could also be used for the JUNCAO cultivation of Ganoderma; but others were not suitable. [Conclusion] G10021 was the optimal strain for JUNCAO cultivation of Ganoderma.

    2012年10期 v.3 95-99页 [查看摘要][在线阅读][下载 234K]
  • Copyright Authorization Statement

    <正>Once a manuscript has been accepted for publication,the author will grant to the editorial department of the journal Medicinal Plant the rights of reproduction,distribution,compilation and information network dissemination,and permit the manuscript to be delegated to the China Academic Journal

    2012年10期 v.3 99页 [查看摘要][在线阅读][下载 60K]
  • Atractylodes macrocephala Koidz. Resources in Anhui Province and Their Development Strategies

    WANG Ning;

    As recorded in ancient literatures, the best A. macrocephala are Shuzhou A. macrocephala and Huizhou A. macrocephala produced in Qianshan of Anhui Province. Their quality is as good as that of Hangzhou A. macrocephala and Yuezhou A. macrocephala in Zhejiang Province, but there are now very few wild resources of Shuzhou and Huizhou A. macrocephala. Although there is a large area cultivation of A. macrocephala in Bozhou of North Anhui, it is not well-known as that produced in Zhejiang. According to the fingerprint study and the chemical components of A. macrocephala, the quality of Huizhou A. macrocephala is as good as that of Zhejiang A. macrocephala, but that of Bozhou A. macrocephala is a little worse. Thus it needs to strengthen the propaganda of Anhui A. macrocephala and the protection of Shuzhou A. macrocephala germplasms, and improve the quality of cultivated A. macrocephala from Bozhou, with the aim to exploit famous Anhui medicines and develop Anhui economy.

    2012年10期 v.3 100-102页 [查看摘要][在线阅读][下载 89K]
  • Medicinal Plant is indexed by CABI,AGRIS,CA and CAJ

    <正>Through the joint efforts of Editorial Department and multitudinous readers,Medicinal Plant eventually passes the extremely strict appraisal, to be indexed by CAJ's full text Database. Since 2011, Medicinal Planthas also been embodied by Chemical

    2012年10期 v.3 2页 [查看摘要][在线阅读][下载 244K]
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