Review

  • Systematic Evaluation of Biyuan Tongqiao Granule in the Treatment of Chronic Sinusitis in Children Based on Meta-analysis

    Zhiyan HUANG;Yongxia GUAN;Qun FENG;Guimin ZHANG;Shili YE;Jingchun YAO;

    [Objectives] To make an evaluation of the efficacy and safety of Biyuan Tongqiao Granule in the treatment of chronic sinusitis in children, and provide evidence-based medical reference for clinical use. [Methods] Databases such as Chinese Biomedical Literature Service System(SinoMed), China Knowledge Infrastructure(CNKI), Wanfang Digital Journal Full-text Database(WF), VIP Database(VIP), EmBase, PubMed, Science Direct, and Cochrane Library were used to collect the clinical randomized controlled trials of Biyuan Tongqiao Granule in children with chronic sinusitis published since the creation of databases. The quality of the included literature was evaluated and the data were extracted. The data was analyzed using Review Manager 5.3 software for meta-analysis, and descriptive analysis was performed for studies that could not be combined. The GRADE method was applied to evaluate the quality of evidence. [Results] A total of 12 studies were included, including 1 645 cases of patients. The analysis results show that compared with the control group, Biyuan Tongqiao Granule has an excellent curative effect on children with chronic sinusitis and has a lower incidence of adverse reactions. The GRADE evidence level indicated that the sinus CT scan lesion score was intermediate evidence, and the visual analog score, endoscopic mucosal morphology score, and total effective rate were low-level evidence. [Conclusions] Biyuan Tongqiao Granule has a significant effect in treating chronic sinusitis in children and does not increase the incidence of adverse reactions, but its efficacy and safety need support of more high-quality studies.

    2020年01期 v.11 1-6+16页 [查看摘要][在线阅读][下载 2467K]
  • An Analysis on the Origin and Development of Nomenclature of Bi Syndrome

    Lamei ZHOU;Donglin HAO;

    The literatures in more than 40 medical history books and classical medical works about TCM name and classification of Bi syndrome were systematically sorted out, summarized and analyzed, which basically reflected the academic origin of Bi syndrome in ancient and modern Chinese medicine. Through the research on the academic origin of the nomenclature of arthralgia syndrome in traditional Chinese medicine, the changes of the nomenclature of arthralgia syndrome in traditional Chinese medicine were clearly grasped, which was of great significance for the further standardization of the nomenclature of arthralgia syndrome.

    2020年01期 v.11 7-10页 [查看摘要][在线阅读][下载 119K]
  • Discussion on the Quality Evaluation Method of Traditional Chinese Medicine Compound Preparations

    Jie LIANG;Yushan ZHOU;Chenxi XIN;Chuanchuan YANG;Jinyu WEI;Dongfang HUANG;Lichun ZHAO;

    As an important representative of the modernization of the Chinese medicine industry, traditional Chinese medicine(TCM) compound preparations are an indispensable part of the development of TCM. The complex ingredients and unclear action mechanism of TCM compound preparations restrict the development of TCM. In line with this problem, the modern TCM research has made extensive remarkable achievements. From the single indicator in the past to multiple indicator, the research on the combination of TCM fingerprints and pharmacodynamics also has made great progress. The introduction of quality evaluation methods such as the "quantitative analysis of multi-components by a single marker", "dose-effect" indicator, "Bioactive Equivalent Combinatorial Components"(BECCs) and TCM quality markers have brought new ideas for quality control of TCM compound preparations.

    2020年01期 v.11 11-16页 [查看摘要][在线阅读][下载 178K]
  • Research Progress on Chemical Constituents and Biological Activity of Kummerowia striata(Thunb.) Schindl.

    Xiucai WU;Yibo XIE;Zhengying SU;

    The purpose of this paper is to review the research progress on the chemical composition and pharmacological action of Kummerowia striata(Thunb.) Schindl., so as to provide reference for the chemical research of natural medicine and the discovery of new drugs. K. striata(Thunb.) Schindl. contains a variety of active constituents, such as flavonoid glycosides, phenols and amino acids, and has a wide range of pharmacological activities, including anti-allergy, analgesia and anti-inflammation, anti-oxidation, anti-tumor and so on. It is mainly used in the folk for the treatment of cold and fever, summer-heat and dampness, vomiting and diarrhea, dysentery and so on.

    2020年01期 v.11 17-20页 [查看摘要][在线阅读][下载 154K]

Chemical Composition of Natural Medicine

  • Preliminary Study on Quality Standard of Pummelo Pericarp from Guangxi

    Chenyan LIANG;Lu ZHANG;Yuhua HUANG;Yunli TANG;Liuyan HE;

    [Objectives] This paper aimed to study the quality standard of pummelo pericarp from Guangxi. [Methods] The medicinal material was identified by microscopy and thin-layer chromatography, and the contents of moisture, total ash, acid-insoluble ash and extract in the medicinal material were determined according to the methods of the Chinese Pharmacopoeia(2010 edition). [Results] The content limits for moisture, total ash, acid-insoluble ash and extract were determined(moisture ≤ 12%; total ash ≤ 6.0%; acid-insoluble ash ≤ 0.6%; water-soluble extract ≥ 43.0%; naringin ≥ 10.00 mg/g). The micro-characteristics of pummelo pericarp were identified, and a thin-layer chromatography identification and content determination method was established. [Conclusions] The established method can provide an experimental basis for the formulation of quality standard for pummelo pericarp.

    2020年01期 v.11 21-23页 [查看摘要][在线阅读][下载 601K]
  • Simultaneous Determination of Effective Components of Gegen Qinlian Tablets by HPLC

    Lili LI;Yifeng LU;Xueyan ZHU;

    [Objectives] To establish a HPLC method for the simultaneous determination of 14 characteristic components in Gegen Qinlian tablets and the content of Gegen Qinlian tablets in 11 manufacturers. [Methods] A Inertsil ODS-3(250 mm×4.6 mm, 5 μm) reversed-phase high performance liquid chromatography column was used with acetonitrile-0.02 mol/L ammonium acetate+0.03% triethylamine solution(adjusted with glacial acetic acid to pH of 4.3) as the mobile phase for gradient elution at a flow rate of 1.0 mL/min. The determination wavelength of 8 components(puerarin, daidzin, liquiritin, baicalin, wogonoside, daidzein, ammonium glycyrrhizinate, baicalein) was 250 nm; the determination wavelength of wogonoside was 280 nm; the determination wavelength of 5 components(epierberine, jatrorrhizine hydrochloride, coptisine, palmatine hydrochloride and berberine hydrochloride) was 346 nm. [Results] There was a good linear relationship between the injection volume of puerarin, daidzin, liquiritin, baicalin, wogonoside, epierberine, jatrorrhizine hydrochloride, coptisine, daidzein, palmatine hydrochloride, ammonium glycyrrhizinate, berberine hydrochloride, baicalein and wogonin and peak area(r>0.999 0) in the range of 146.26-585 0.24, 24.13-965.04, 18.45-738.00, 79.18-316 7.32, 9.57-382.80, 4.76-190.40, 2.57-102.80, 13.41-536.40, 10.60-424.00, 11.33-453.22, 12.08-483.20, 46.73-186 9.25, 20.28-811.20, 12.11-484.50 ng, respectively; the average recovery rates(n=6) were 2.18%, 1.79%, 1.81%, 1.68%, 2.27%, 2.13%, 1.96%, 1.07%, 0.93%, 0.61%, 2.92%, 0.77%, 2.79% and 0.62%, respectively; the precision, repeatability and stability were good, and the RSD was less than 3%. This method was used to determine 16 batches of Gegen Qinlian tablets produced by 11 enterprises. Wogonoside was not detected in a lot of samples, but 14 components were detected for other enterprises, but the content was different. [Conclusions] The method was accurate and feasible and could be used for the overall quality control of this variety.

    2020年01期 v.11 24-28+36页 [查看摘要][在线阅读][下载 816K]

Pharmacological and Toxicological Analyses

  • Preliminary Experimental Study on Anti-inflammatory Effect of Extract from Excoecaria cochinchinensis Lour. as a National Medicine

    Jiangcun WEI;Silu HE;Haisheng ZENG;Dongmei HUANG;Chengtong LIU;Qiji ZHOU;Honghan QIN;

    [Objectives] To study the anti-inflammatory effects of water extract and ethanol extract from Excoecaria cochinchinensis Lour. [Methods] The acute and chronic inflammation models of mouse paw edema induced by cotton ball granuloma and carrageenan were established respectively, and the anti-inflammatory effects of water and ethanol extract from E. cochinchinensis Lour. on acute and chronic inflammation in mice were evaluated. The experimental mice were equally divided into three groups of high, medium and low dose of E. cochinchinensis Lour.(60, 40 and 20 g/kg), dexamethasone(0.006 g/kg) group and blank group(distilled water). In the cotton ball granuloma test in mice, the mice were given intragastric administration continuously for 7 d after cotton ball implantation. In the experiment of inflammation induced by carrageenan, the mice in each group were given intragastric administration once a day for 7 d, and the inflammatory model was made after the last administration. [Results] The granuloma inhibition rates of mice in the high, medium and low dose groups of water extract and ethanol extract from E. cochinchinensis Lour were significantly lower than those in the blank group(P<0.05). The degree of paw edema in each dose group of water and ethanol extract from E. cochinchinensis Lour. was significantly lower than that in the blank group(P<0.05). [Conclusions] The water and ethanol extracts from E. cochinchinensis Lour. had significant anti-inflammatory effects.

    2020年01期 v.11 29-31页 [查看摘要][在线阅读][下载 133K]
  • A Preliminary Study on Anti-inflammatory and Analgesic Effects of Millettia speciosa and Tinpspora sinensis and Their Compatibility

    Shibo ZHAO;Suoyi HUANG;Xinpeng CHEN;Huanhui DENG;Zhi PAN;Kairen XIE;Haoming YU;Fen MO;

    [Objectives] To study the anti-inflammatory and analgesic effects of Millettia speciosa and Tinpspora sinensis and their compatibility. [Methods](i) In the glacial acetic acid writhing experiment, 60 SPF-grade Kunming mice were adopted, and the mice were randomly divided into 6 groups at male-female ratio of 1∶1, namely, the blank control group, M. speciosa group, T. sinensis group, M. speciosa compatible with T. sinensis group at the ratio of 1∶2(expressed as 1∶2 compatibility group), M. speciosa compatible with T. sinensis group at the ratio of 1∶1(expressed as 1∶1 compatibility group), and M. speciosa compatible with T. sinensis group at the ratio of 2∶1(expressed as 2∶1 compatibility group), 12 mice for each group. Mice of the experimental groups were administered at a dose of 20 mL/kg, and the corresponding concentration of the Chinese medicine extract was given at 1 g/mL. The control group was administered with an equal volume of 0.9% physiological saline, and was intragastrically administered once every 24 h for 14 d. After intragastric administration for one hour on day 14, intraperitoneal injection of 0.5% glacial acetic acid solution was performed to induce pain.(ii) In the hot plate experiment, 60 Kunming female SPF mice were adopted, grouped, intragastrically administered with the same glacial acetic acid writhing experiment for 14 d. After intragastric administration for one hour on day 14, the mice were placed on a hot plate apparatus at(55±0.5) ℃. to measure the time of licking their hind feet.(iii) In the anti-inflammatory experiment, 60 Kunming SPF mice were adopted, grouped, intragastrically administered with the same glacial acetic acid writhing experiment for 14 d. After intragastric administration for one hour on day 14, xylylene was administered to the left ears of mice at a dose of 50 μL/piece to induce inflammation. [Results](i) In the glacial acetic acid writhing experiment, compared with the blank control group, the experimental group showed analgesic effects. Specifically, M. speciosa group, T. sinensis group, 1∶2 compatibility group, 1∶1 compatibility group, 2∶1 compatibility group showed significant effect(P<0.05), the writhing inhibition rate was 17.65%, 20.59%, 29.41%, 26.47%, and 44.12%, respectively, and 2∶1 compatibility group showed the most significant analgesic effects.(ii) In the hot plate experiment, compared with the control group, all experimental groups showed analgesic effect. Specifically, M. speciosa group, T. sinensis group, 1∶2 compatibility group, 1∶1 compatibility group, 2∶1 compatibility group showed significant effect(P<0.05), the pain threshold improvement rates were 16.13%, 14.55%, 14.96%, 29.95%, and 58.68%, respectively, and 2∶1 compatibility group showed the most significant analgesic effect.(iii) In the anti-inflammatory experiment, the swelling degree of the 1∶2 compatibility group was significantly different from that of the blank control group, M. speciosa group, T. sinensis group(P<0.05). and 1∶2 compatibility group showed the most significant anti-inflammatory effect. [Conclusions] M. speciosa, T. sinensis, and their compatibility had anti-inflammatory and analgesic effects. The 2∶1 compatibility group had the best analgesic effects, and 1∶2 compatibility group had the best anti-inflammatory effects.

    2020年01期 v.11 32-36页 [查看摘要][在线阅读][下载 175K]
  • Effects of Paeonol Combined with Panax Notoginseng Saponins on Nrf2/ARE Pathway in Rats with Diabetic Cardiomyopathy

    Yanfang FAN;Xiaoli YAN;Zhuoya JIA;Kaihang SHI;Kai CAO;

    [Objectives] To investigate whether paeonol(Pae) combined with panax notoginseng saponins(PNS) can protect the myocardium of rats with diabetic cardiomyopathy(DCM) through improving the antioxidant capacity of the rats by activating the Nrf2/ARE pathway. [Methods] The rats were fed with high-fat and high-sugar diet for 6 weeks, and combined with intraperitoneal injection of small-dose STZ to build a type II diabetes model; the model rats were randomly divided into a model group, a Pae group of 80 mg/kg, and a PNS group of 100 mg/kg, a Pae 80 mg/kg+PNS 100 mg/kg, and a metformin group 157.5 mg/kg; rats of normal group and model group were injected with an equal volume of sodium carboxymethyl cellulose(1%), 10 rats in each group. After 8 weeks, 3 rats in the model group were taken for histopathological examination. Changes in myocardial fibrosis and myocardial collagen formation indicated that the building of DCM model was successful. qRT-PCR and Western blot were used to detect the expression levels of Nrf2, HO-1, Col-I, and Col-III in myocardial tissue of each group of rats. [Results] Compared with the normal group, the mRNA and protein expression levels of Nrf2 and HO-1 in myocardial tissue of the DCM group rats were significantly reduced, and the mRNA and protein expression levels of Col-I and Col-III were significantly increased; compared with the DCM group, the mRNA and protein expression levels of Nrf2 and HO-1 in the myocardial tissue of each drug group increased to varying degrees, and the combined drug group increased more significantly than that of the single drug group; the mRNA and protein expression levels of Col-I and Col-III were reduced to varying degrees, and the combined drug group declined more significantly than that of the single drug group. [Conclusions] Paeonol combined with panax notoginseng saponins can up-regulate the expression of Nrf2 and HO-1 in myocardial tissue, inhibit the expression of type I and type III collagen. The mechanism may be related to improving the DCM myocardial fibrosis through activating the Nrf2/ARE pathway.

    2020年01期 v.11 37-39+43页 [查看摘要][在线阅读][下载 480K]
  • Anti-tumor Activity of Derivatives of Protopanaxadiol Prepared with Acid Anhydrides

    Jinna ZHOU;Rongping ZHANG;Cheng ZOU;Weimin YANG;Wenyue DUAN;Chen CHEN;Jianlin HU;

    [Objectives] This paper aimed to prepare derivatives of protopanaxadiol from Panax notoginseng(Burk.) FH Chen with acid anhydrides and study their anti-tumor activity. [Methods] The 3-hydroxyl group of protopanaxadiol was subjected to structural modification and reacted with acid anhydrides to prepare derivatives, in order to improve the anti-tumor activity of protopanaxadiol. None of the five compounds designed and synthesized had been reported in the literature, and they were novel compounds. The anti-tumor activity of the derivatives was studies using MTS method. Taking cisplatin and paclitaxel as positive control drugs, the bioactivity of the compounds 1-5 on anti-tumor cell lines(HL-60 cells, SMMC-7721 cells, A-549 cells, MCF-7 cells and SW480 cells) in vitro was screened. [Results] The compound 5 showed inhibitory effect on HL-60 cells, SMMC-7721 cells and A-549 cells. [Conclusions] The acid anhydride esterification method is simple to operate and easy to control. This study has reference value for the structural modification and anti-tumor activity research of protopanaxadiol from P. notoginseng(Burk.) FH Chen.

    2020年01期 v.11 40-43页 [查看摘要][在线阅读][下载 333K]
  • Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease

    Ying HAO;Hongwei ZHONG;Yuanyun GU;Hui CHEN;

    [Objectives] This study aimed to study the effects of Shenge Yifei capsule on the TGF-β1/Smad signaling pathway in rats with chronic obstructive pulmonary disease(COPD). [Methods] Ten rats were randomly selected as the control group, and the other 40 rats were selected for modeling by fumigation combined with Klebsiella pneumoniae infection. A total of 38 rats were successfully modeled. They were randomly divided into model group(8 rats), low-dose Shenge Yifei capsule group(10 rats), high-dose Shenge Yifei capsule group(10 rats) and theophylline group(10 rats) in accordance with the principle of half male and half female. The rats in the model and control groups were given with distilled water by gavage, and the rats in the drug administration groups were given with corresponding drugs. The TGF-β1 level in the serum, and the expression levels of TGF-β1, Smad2, Smad3 and Smad7 and TGF-β1, Smad3 and Smad7 in airway tissues were detected. [Results] After 12 weeks, the serum TGF-β1 levels of the theophylline group and high-dose Shenge Yifei capsule group were lower than that of the low-dose Shenge Yifei capsule group(P<0.05). The expression levels of TGF-β1 and Smad3 in the theophylline group and high-dose Shenge Yifei capsule group were lower than that in the low-dose Shenge Yifei capsule group(P<0.05). The expression levels of TGF-β1 and Smad3 in the high-dose Shenge Yifei capsule group were lower than those in the low-dose Shenge Yifei capsule group and theophylline group(P<0.05). The expression levels of Smad7 and the proteins in the model group were lower than those in the other groups(P<0.05). The expression levels of Smad7 in the theophylline group and high-dose Shenge Yifei capsule group were higher than that in the low-dose Shenge Yifei capsule group(P<0.05). After 18 weeks, no significant difference was found in serum TGF-β1 level among the theophylline group and low and high-dose Shenge Yifei capsule groups(P>0.05). The expression levels of Smad7 and the proteins in the model group were lower than those in the other groups. The expression level of Smad7 in the high-dose Shenge Yifei capsule group was lower than that in the theophylline group(P<0.05). [Conclusions] Shenge Yifei capsule can regulate the TGF-β1/Smads signaling pathway. They can down-regulate the expression of TGF-β1, Smad2 and Smad3 and up-regulate the expression of Smad7, reducing the degree of airway modeling, delaying the development of COPD disease. Conventional high-dose Shenge Yifei capsule is more effective in inhibiting the expression of Smad2.

    2020年01期 v.11 44-47页 [查看摘要][在线阅读][下载 729K]
  • Effects of Suoquan Yishen Formula on Mice with Diabetic Nephropathy (DN)

    Fan YANG;Yujian YAO;Kun NIU;Ling HUANG;Man XIAO;Guankui DU;Xiang LI;Qian NIU;Yali NI;Can TANG;Yiqiang XIE;

    [Objectives] The purpose of this paper was to study the effects of Suoquan Yishen formula on mice with diabetic nephropathy(DN). [Methods] The mice were randomly divided into normal group, model group, irbesartan group, and high and low-dose Suoquan Yishen formula groups. The blood glucose level, BUN, Scr excretion and GSH activity and CAT activity were detected. [Results] The blood glucose levels of mice in the irbesartan group and high and low-dose Suoquan Yishen formula groups decreased. The BUN, PRO and Scr levels were higher and the GSH and CAT activity was lower in the model group compared with the normal group. After administration of Suoquan Yishen formula, the BUN, PRO and Scr levels of the DN mice decreased significantly, and the GSH and CAT activity increased significantly. [Conclusions] Suoquan Yishen formula can reduce blood glucose level, reduce PRO, BUN and Scr levels, and increase CAT and GSH activity in DN mice, thus protecting the ND mice.

    2020年01期 v.11 48-50页 [查看摘要][在线阅读][下载 135K]
  • Effect of Total Flavonoids from Phyllanthus emblica L. on Tumor Proliferation and Immune Function

    Wenya CHEN;Erchu GUO;Zhenguo ZHONG;

    [Objectives] To explore the effect of total flavonoids from Phyllanthus emblica L. on tumor proliferation and immune function. [Methods] The effects of total flavonoids of P. emblica L. on the proliferation of 6 different tumor cell lines(human hepatoma cell line HepG-2, human cervical cancer cell line Hela, human gastric cancer cell line SGC7901, human nasopharyngeal carcinoma cell line CNE-2, human lung cancer cell line H460 and human ovarian cancer cell line A2780) were compared by SRB method. The effect of total flavonoids from P. emblica L. on the proliferation of mouse lymphocytes induced by concanavalin(ConA) or lipopolysaccharide(LPS) in vitro was detected by CCK-8 method. [Results] The results of SRB assay showed that compared with the normal group, the total flavonoids of P. emblica L. had obvious inhibitory effect on 6 kinds of tumor cells. Among them, the inhibitory effect on H460 cells and CNE-2 cells was the most significant, and the IC50 was(471.36±50.66),(463.26±40.75) μg/mL, respectively. The high dose group of total flavonoids from P. emblica L. had the same inhibitory effect as the positive drug 5-Fu. The results of CCK-8 assay showed that compared with the blank group, the total flavonoids of P. emblica L. significantly inhibited the proliferation of mouse lymphocytes induced by ConA or LPS(~*P<0.05,~(**)P<0.01). [Conclusions] The total flavonoids of P. emblica L. had significant anti-tumor activity.

    2020年01期 v.11 51-54页 [查看摘要][在线阅读][下载 451K]
  • Protective Effect and Action Mechanism of Modified Ershiwuwei Songshi Pills on Chronic Liver Injury Induced by CCL_4

    Xican MA;Shuai YANG;Ke BA;Zhilong SHI;Jian GU;

    [Objectives] To study the protective effect of Ershiwuwei Songshi Pills on chronic liver injury induced by carbon tetrachloride(CCL_4) in rats before and after the modification conforming to the compatibility theory of Tibetan medicine, and to explore its action mechanism. [Methods] Male Wistar rats were randomly divided into the blank control group, model group, Hugan tablets group(0.490 g/kg), Ershiwuwei Songshi Pills group(0.117 g/kg), and Modified Ershiwuwei Songshi Pills group(removing cinnabaris, Aristolochia contorta, and Aconitum naviculare, 0.105 g/kg). Except the blank group, the remaining groups were injected subcutaneously with 20% carbon tetrachloride olive oil solution every 3 d, and modeled for 6 weeks. During this time, intragastrically administered corresponding drugs. Six weeks later, blood was taken from the femoral artery, and the rats were killed through dislocating the cervical spine, the liver was taken, and the content of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) was determined. Then, liver fibrosis indicators tumor necrosis factor-α(TNF-α), nuclear factor-κB(NF-κB), interleukin-6(IL-6) and interleukin-1β(IL-1β) were detected by immunohistochemical method. [Results] Compared with the model group, the pathological map of the liver section showed that liver injury was improved in each administration group. The serum ALT and AST contents in rats of each administration group were significantly reduced(P<0.05), and the protein expressions of NF-κB, TNF-α, IL-1β and IL-6 in liver tissue were also reduced by varying degrees(P<0.05). [Conclusions] Ershiwuwei Songshi Pills and its modification group have a protective effect on liver injury induced by carbon tetrachloride. The modified prescription conforms to the compatibility rules of Tibetan medicine. The mechanism may be related to reducing the damage caused by inflammatory factors through regulating the role of inflammatory signaling pathway. Thus, it can be used as a reference for future optimization proposals.

    2020年01期 v.11 55-58页 [查看摘要][在线阅读][下载 461K]
  • Effects of Losartan on Cell Apoptosis and Expression of Caspase-3 and JNK Proteins in Kidney Tissue in Renal Interstitial Fibrosis Rats

    Lu BIAN;Chunhua LUO;Weifeng FENG;

    [Objectives] To investigate the effects of losartan on cell apoptosis and the expression of caspase-3 and JNK proteins in kidney tissue in the adenine-induced renal fibrosis rats. [Methods] Thirty Wistar rats were randomly divided into three groups: control group(n=10), model group(n=10) and losartan group(n=10). The rats in the control group received saline, while those in the model group and losartan group both received adenine by gavage, for 21 d. After the renal interstitial fibrosis model was established, the rats in the losartan group were treated with losartan[10 mg/(kg·d)], while the rats in the control group and the model group rats were administered with the same amount of saline. The course of treatment was 30 d. Finally, the renal function, blood urea nitrogen(BUN), serum creatinine(Scr), creatinine clearance rate(Ccr) and the pathological morphology of the rats were detected. The apoptosis of renal tubular epithelial cells was tested by TUNEL. The caspase-3 and JNK protein expression was tested by Western blotting. [Results] After administering adenine for 21 d, the BUN, 24 MTP and kidney/body weight in the model group were increased, significantly higher than the control group(P<0.01), and the Ccr was remarkably decreased(P<0.01), signifying that the renal interstitial fibrosis model was successfully built. After treating with losartan for 30 d, the Scr, BUN, and 24 MTP were significantly decreased(P<0.01), and the Ccr was significantly increased in the losartan group(P<0.01). In addition, in comparison to the model group, renal tubular epithelial apoptosis was decreased and caspase-3 and JNK expression was downregulated in the losartan group(P<0.05). [Conclusions] Losartan can reduce the adenine-induced elevation of Scr, BUN and 24 hMPT, increase Ccr, improve general condition of renal interstitial fibrosis in rats and ameliorate the progression of chronic kidney failure(CKD). The effectiveness of losartan is probably due to its ability to regulate caspase-3, JNK protein expression and attenuate renal cell apoptosis.

    2020年01期 v.11 59-62+66页 [查看摘要][在线阅读][下载 1950K]
  • Effects of Different Processed Products of Radix Codonopsis on Intestinal Flora of Rats with Spleen Deficiency

    Jiabao MA;Zhengteng YANG;Jiangcun WEI;Jian CUI;Song CHEN;Dongmei HUANG;Lifu WEI;

    [Objectives] This study aimed to observe the effects of different processed products of Radix Codonopsis on intestinal flora in rats with spleen deficiency. [Methods] Rat models with spleen deficiency were established by bitter-cold purgation method with Radix et Rhizoma Rhei. Normal group, model group, Radix Codonopsis group, fried Radix Codonopsis group, rice-fried Radix Codonopsis group, honey-fried Radix Codonopsis group and bran-fired Radix Codonopsis group were designed. After subjecting to corresponding treatments, the changes in the quantity of intestinal microorganisms of the rats were detected. [Results] There was no significant change in the quantity of intestinal microorganisms of the rats in the normal group and model group. The rats in the rice-fried Radix Codonopsis group were administered after successful modeling, and the abundance of Bifidobacterium and Lactobacillus in the intestines of the rats increased, and the quantity of Escherichia coli and Staphylococci reduced. The rats in the Radix Codonopsis group, fried Radix Codonopsis groups, honey-fried Radix Codonopsis group and bran-fried Radix Codonopsis group were administered after successful modeling, and the abundance of Bifidobacterium and Lactobacillus in the intestines of the rats increased(the increases were smaller than those in the rice-fried Radix Codonopsis group), and the abundance of E. coli and Staphylococci reduced, close to normal levels. [Conclusions] Different processed products of Radix Codonopsis have obvious regulation effect on intestinal flora of rats with spleen deficiency, and the regulation effect of rice-fried Radix Codonopsis on rats with spleen deficiency is better than that of Radix Codonopsis, fried Radix Codonopsis, honey-fried Radix Codonopsis and bran-fried Radix Codonopsis.

    2020年01期 v.11 63-66页 [查看摘要][在线阅读][下载 286K]

Processing of Traditional Chinese Medicineand Quality Control

  • Quality Evaluation of Yi Medicine "Qibujing" Based on Determination of Water Content and Ash Content

    Xuexue LI;Hongna SU;Ying LI;Xiaoxia CAI;Risha WEIZE;Zhengming YANG;Yingfen HAO;Yuan LIU;

    [Objectives] To determine the water content, total ash content and acid-insoluble ash content of Yi medicinal material "Qibujing", so as to provide experimental basis for establishing the Quality Standard(Draft) of Yi Medicinal Material "Qibujing" in Sichuan Province. [Methods] According to the second method(drying method) in 0832 water content determination method and 2301 ash content determination method in Chinese Pharmacopoeia(2015 Edition), it was determined, respectively, and the data were processed by IBM SPSS Statistics 26 and DPS 7.05 data processing system. [Results] All the 14 batches of sample met the stipulation that the water content should not exceed 12.0%, the total ash content should not exceed 8.0%, and the acid-insoluble ash content should not exceed 1.5% under "Guan Zhong-(Qibujing)" in the 2005 edition of Yunnan Traditional Chinese Medicine Standard. There was a negative correlation between water content, acid-insoluble ash content and altitude, and a positive correlation between total ash content and altitude. [Conclusions] It is suggested that Quality Standard(Draft) of Yi Medicinal Material "Qibujing" in Sichuan Province stipulate that the water content should not exceed 12.0%, the total ash content should not exceed 8.0%, and the acid-insoluble ash content should not exceed 1.5%.

    2020年01期 v.11 67-68+92页 [查看摘要][在线阅读][下载 123K]
  • Effects of Different Storage Methods and Storage Time on the Quality of Codonopsis pilosula

    Shuxian XIAO;Zhaoxia SONG;Yuqiang FANG;Xufeng WANG;Feihe LI;Zhenhong LEI;Yulong WANG;

    [Objectives] To study the effects of different storage methods and storage time on the quality of Codonopsis pilosula. [Methods] Experiment was performed by setting three different storage methods and times. [Results] When stored for 12 months, the extract content in the natural storage, conventional sealing, and controlled atmosphere storage was slightly lower than 77.32% before storage, and decreased by 2.82%, 3.62%, and 1.71%, respectively. When stored for 18 months, the extract content was 74.14%, 73.21%, and 72.08%, decreased by 4.11%, 5.31%, and 6.78%, respectively compared with that before storage, all of which met the specified content in Chinese Pharmacopoeia. [Conclusions] The three storage times had significant effects on moisture and extract content of C. pilosula, but had no significant effects on atractylenolide III. With the extension of storage time, the moisture, extract, and content declined, so the storage time theoretically should not exceed 12 months.

    2020年01期 v.11 69-72+79页 [查看摘要][在线阅读][下载 817K]
  • Effects of Pb on Rhizosphere Soil Enzyme Activity and Chemical Constituents of Achyranthes bidentata Blume

    Xinmin WANG;Zhihua WEI;Lihong LI;Qingmei QIAO;

    [Objectives] The purpose of this study was to investigate the effects of Pb on rhizosphere soil enzyme activity and chemical constituents of Achyranthes bidentata Blume. [Methods] A. bidentata Blume plants were cultivated in self-made polyvinyl chloride(PVC) pots. The soil was added with different levels of Pb(0, 200, 400, 600, 800 and 1 000 mg/kg air-dried soil) to investigate the effects of Pb on dry mass, active ingredients(oleanolic acid and ecdysterone) and rhizosphere soil enzyme activity of A. bidentata Blume. [Results] The root dry mass of A. bidentata Blume cultivated in the soil with Pb level above 400 mg/kg significantly reduced. The Pb residues in the A. bidentata Blume. plants growing in the soil with Pb level below 400 mg/kg complied with national standard. The contents of oleanolic acid and ecdysterone in A. bidentata Blume growing in the soil with Pb level above 600 mg/kg declined significantly. At the Pb level of 1 000 mg/kg, the activity of urease was inhibited significantly. The activity of phosphatase was inhibited in the presence of Pb in the soil. The activity of sucrase was activated in the soil with Pb level below 400 mg/kg, and was inhibited in the soil with Pb level above 400 mg/kg. [Conclusions] This study has important guiding significance for the reasonable selection of planting base for A. bidentata Blume and the guarantee of its yield and quality.

    2020年01期 v.11 73-75页 [查看摘要][在线阅读][下载 447K]
  • Evaluation of Wild Paeonia veitchii Lynch from Sichuan Based on Moisture, Ash and Extract Contents

    Fang XIAO;Xuexue LI;Jixiu SHEN;Xiaoxia CAI;Zhengming YANG;Yuan LIU;

    [Objectives] The research aimed to determine the contents of moisture, ash and extract of wild Paeonia veitchii Lynch produced in Sichuan, so as to provide the basis for evaluating quality of wild P. veitchii Lynch from Sichuan. [Methods] Moisture, ash and extract contents of wild P. veitchii Lynch were respectively determined according to the fourth part of the Chinese Pharmacopoeia of 2015 version, and correlation analysis and TOPSIS analysis were used for data processing. [Results] 12 batches of samples all corresponded with the provision that moisture of P. veitchii Lynch should not exceed 14.0% in the Standard of Traditional Chinese Medicine in Sichuan Province of 2010 version; 11 batches of samples all corresponded with the provisions that total ash and acid insoluble ash of P. veitchii Lynch should not exceed 7.0% and 1.0% in the Standard of Traditional Chinese Medicine in Sichuan Province of 2010 version; average content of alcohol-soluble extract by hot dipping from 12 batches of samples was 42.29%, which was not less than 30.0%. In P. veitchii Lynch, moisture and acid insoluble ash showed positive correlation with altitude, while total ash and extract content were negatively correlated with altitude. Taking moisture, ash and extract as evaluation indexes, No.9 P. veitchii Lynch sample was the best. [Conclusions] Moisture and ash of P. veitchii Lynch corresponded with the provisions of the Chinese Pharmacopoeia of 2015 version and the Standard of Traditional Chinese Medicine in Sichuan Province of 2010 version, while extract content was higher than provision of the standard. The research could provide reference basis for rational utilization and quality evaluation of P. veitchii Lynch resources.

    2020年01期 v.11 76-79页 [查看摘要][在线阅读][下载 145K]

Germplasm Resources and Cultivation

  • Effects of 12% Chlorothalonil and 12% Procymidone Smoke Agents on Physiology and Yield of Quinoa in Greenhouse

    Wei LU;Yiming LIU;Jianhan HE;Meiyan PEI;Shiping WANG;Zhimin WEI;Chuan LU;

    [Objectives] To study the effects of 12% chlorothalonil and 12% procymidone smoke agent on physiology and yield of quinoa in greenhouse. [Methods] The chlorophyll content and photosynthesis rate of quinoa were determined by using the conventional application method of smoke agent at the dosage of 3 kg/ha. Finally, the total yield and increased yield of quinoa were calculated. [Results] The chlorophyll content and photosynthesis rate of leaves were all increased by two kinds of smoke agents, and the photosynthesis rate was also increased significantly. [Conclusions] These two smoke agents can promote the growth of quinoa, including the increase of chlorophyll and the enhancement of photosynthesis, and its further mechanism needs to be further discussed.

    2020年01期 v.11 80-81页 [查看摘要][在线阅读][下载 112K]
  • Effects of GA_3 and NAA on Fatty Acid Degradation in Germination and Seedling Growth of Cassia obtusifolia L. Seed

    Die FU;Shiqiong LUO;Zhannan YANG;Yuanshuai WANG;Haitao YE;

    [Objectives] To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L. [Methods] The fatty acid compositions of germinations and seedling growths of C. obtusifolia seeds treated with different concentrations of gibberellin(GA_3) and naphthalene acetic acid(NAA) were analyzed using gas chromatograph-mass spectrometer(GC-MS), and the germination rates, root lengths, plant heights and fresh weights of their seedlings were analyzed. [Results] The contents of eicosenoic acid, α-linoleic acid, arachidic acid, eicosadienoic acid and eicosapentaenoic acid of C. obtusifolia seeds treated with different concentrations of GA_3 and NAA decreased with germination time. There were significant differences between the ratios of germinations of C. obtusifolia seeds treated with different concentrations of GA_3 and NAA. GA_3 and NAA of different ratio treatments could promote seedling heights and fresh weights of C.obtusifolia, but increases of seedling heights and fresh weights were different. Eicosenoic acid, α-linoleic acid, arachidic acid, eicosadienoic acid and eicosapentaenoic acid were remarkably negative correlation with seedling heights and fresh weights. [Conclusions] The fatty acid components of C. obtusifolia seed germination and growth of their seedlings were affected. It indicated that GA_3 and NAA treatments can affect fatty acid metabolism, the germination rates, root lengths, plant heights and fresh weights of C. obtusifolia. It is intended to provide a theoretical basis and technological reference for standardized cultivated techniques of C. obtusifolia.

    2020年01期 v.11 82-87页 [查看摘要][在线阅读][下载 1092K]

Others

  • Application of Curcumin Microneedles

    Zifan ZHAO;Enlong WANG;Lili HE;

    [Objectives] To prepare curcumin solid dispersion(Cur SD) by the melting method, using povidone(PVP K30), polyethylene glycol 6000(PEG 6000), and poloxamer 188(block polyether F-68, poloxamer 188, F68) as the carrier matrix, the body was directly inverted and pressed to make microneedles, and the dissolution was examined. [Methods] The dissolution in 0.2% sodium lauryl sulfate(SDS) solution within 120 min was studied, and the objects were identified by scanning electron microscopy, differential scanning calorimetry and infrared spectroscopy. [Results] The cumulative dissolution rate of 10% solid dispersion microneedles reached more than 60% in 120 min. The results of object identification also showed that curcumin was dispersed in the carrier matrix in an amorphous state. [Conclusions] Microneedles were well formed and curcumin solid dispersion significantly improved the dissolution of curcumin in 120 min.

    2020年01期 v.11 88-92页 [查看摘要][在线阅读][下载 1929K]

  • AGRIS

    <正>AGRIS (The Agricultural Sciences and Technology) is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO. It embodies continued publications,documents,corpuses,books,technology reports,patents,maps,meeting papers,and other literatures,which are collected by 135 countries and regions,146

    2020年01期 v.11 47页 [查看摘要][在线阅读][下载 210K]
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