Chemical Composition of Natural Medicine

  • HS-SPME-GC-MS Analysis on Volatile Components in Different Cultivars and Different Parts of Artemisia argyi

    Li CAO;Jinqing LU;Xin YE;

    [Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific basis for comprehensive development and rational utilization of Artemisia argyi. [Methods]Firstly,HS-SPME was used to extract the volatile components from the leaves,seeds,and stems of seven-tip Artemisia argyi,five-tip Artemisia argyi,and golden Artemisia argyi. Their chemical composition was analyzed by gas chromatographymass spectrometry( GC-MS),and the relative mass fraction of each component was determined by area normalization method. [Results] For the same variety of Artemisia argyi,the content of volatile components in leaves was higher than in seeds and stems except camphor,and the content of volatile components in seven-tip Artemisia argyi leaves was higher than in five-tip Artemisia argyi and golden Artemisia argyi leaves.HS-SPME-GC-MS analysis showed that eucalyptol,camphor,thujone,α-pinene,1-caryophyllene and caryophyllene oxide with higher content were detected in different parts of the different cultivars of Artemisia argyi. [Conclusions]Seven-tip Artemisia argyi leaves were the best medicinal parts of the three cultivars,followed by seven-tip Artemisia argyi seeds.

    2017年04期 v.8 1-5+9页 [查看摘要][在线阅读][下载 789K]
  • Study on the Variation in Main Chemical Composition of Blumea balsamifera in Different Growth Stages

    Mei HUANG;Ce CHEN;Liwei LIU;Zhenxia CHEN;

    [Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the plant was determined by gas chromatography( GC)with methyl salicylate as internal standard,and total flavonoids were determined by the Aluminum nitrate colorimetry method of Ultraviolet-Visible spectrophotometry. The analysis of variance was used for statistical analysis of the l-borneol and total flavonoids of B. balsamifera in different months and different plant age.[Results]There were differences in chemical ingredients of B. balsamifera in different months of the year.The content of l-borneol in October was the highest,but there was no significant difference from August to December in l-borneol content( P >0. 05). l-borneol content of B. balsamifera in three ages was the highest compared with other ages,and there was a significant difference with B. balsamifera in one year or less( P < 0. 05). Relatively speaking,total flavonoids in June,August and November were higher; total flavonoids content of B. balsamifera in two ages was the highest compared with other ages,and there was a significant difference with other ages( P <0. 05).[Conclusions]If l-borneol is taken as an indicator,for extracting Aipan( l-borneol),it can be harvested from September to midDecember,but October is the best. And it can be harvested at least three years. If the content of total flavonoids is taken as an indicator,it should be harvested in November. And it can be harvested 2-3 years.

    2017年04期 v.8 6-9页 [查看摘要][在线阅读][下载 956K]
  • GC-MS Analysis of Liposoluble Constituents from Buds,Flowers and Fruits of Millettia speciosa Champ.

    Maoyuan WANG;Qing YANG;Qinglong WANG;Xiaoxia YAN;Jianrong WANG;Zhunian WANG;

    [Objectives] To study the material foundation of liposoluble constituents from buds,flowers and fruits of Millettia speciosa Champ.and provide a reference for the development of flowers and fruits of Millettia speciosa Champ.[Methods]The liposoluble constituents were extracted from buds,flowers and fruits of Millettia speciosa by Soxhlet extraction and solvent extraction method,and analyzed by GC-MS.[Results]24 compounds were identified from the liposoluble constituents of buds,accounting for 88. 31 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 52. 00%) and alcohols compounds( 17. 46%); 29 compounds were identified from the liposoluble constituents of flowers,accounting for 91. 38 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 60. 64%) and alcohols compounds( 17. 17%); 32 compounds were identified from the liposoluble constituents of fruits,accounting for 80. 01 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 32. 56%),phenyl and its derivatives compounds( 22. 46%) and fatty acids compounds( 12. 54%). 6 compounds were common in buds,flowers and fruits. [Conclusions] Although there were some differences in liposoluble constituents from flowers,fruits,leaves and roots of Millettia speciosa Champ.,the different parts of Millettia speciosa Champ. had development value.

    2017年04期 v.8 10-14页 [查看摘要][在线阅读][下载 705K]

Processing of Traditional Chinese Medicine and Quality Control

  • Preliminary Study on HPLC Fingerprint of Thlaspi arvense L.

    Jiamin MENG;Hanmei JIANG;Jinqing LU;Kun ZHOU;Yushuo XIAO;

    [Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.

    2017年04期 v.8 15-19页 [查看摘要][在线阅读][下载 1198K]
  • HPLC Fingerprint Chromatogram of Tetracera asiatica Produced in Guangxi

    Fangfang DA;Zhonghua DAI;Hua ZHU;Jianbei TENG;

    [Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) method was used. Phenomenex C18( 5 μm,4. 6 ×250 mm),acetonitrile-0. 1% phosphoric acid water,gradient elution,flow rate 1 m L/min,detection wavelength 214 nm,and column temperature 25 ℃. The fingerprint chromatogram of 10 batches of samples of Tetracera asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi,determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was > 0. 9.[Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica.

    2017年04期 v.8 20-22页 [查看摘要][在线阅读][下载 724K]
  • Comparative Analysis of Fingerprint Spectra of Fruits and Leaves of Prunus mume Collected at Various Altitudes in Different Months

    Qian JIN;Xue ZHAI;Fang XIAO;Zhe LIU;Yongxia KE;Yuan LIU;

    [Objectives] To study characteristic fingerprint spectra of fruits( its stones were removed) and leaves of Prunus mume collected at various altitudes in different regions of Jinchuan County,the Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province,China in different months by using Ultra Performance Liquid Chromatography( UPLC).[Methods]The evaluation system of similarity of chromatographic fingerprint spectra of traditional Chinese medicine and SPSS19. 0 were used to assess their similarity and to establish the reasonable reliable common model of chromatographic fingerprints,and the quality of the medicinal materials was evaluated.[Results] Seen from the fingerprint spectra of fruits( its stones were removed) and leaves of P. mume collected at different altitudes in various months,there were 15 common peaks in the fingerprint spectra of the fruits( its stones were removed),and the similarity of the 28 samples was higher than 0. 900,showing that there were small differences between the samples of the fruits( its stones were removed) in chemical components; there were 10 common peaks in the fingerprint spectra of the leaves,and the similarity of the 28 samples was also higher than 0. 900,indicating that there were also small differences between the samples of the leaves in chemical components.[Conclusions] The method of using fingerprint spectra to control the quality of fruits and leaves of P. mume was simple and stable and had strong specificity and good repeatability.

    2017年04期 v.8 23-26+31页 [查看摘要][在线阅读][下载 2155K]
  • Effects of Different Drying Methods on the Quality of Micronized Chinese Wolfberry Powder

    Guangpeng LIU;Juan WANG;Fengtao ZHU;Yan ZHAO;Bangguo GE;Peini ZHAO;Fatao HE;

    [Objectives] To study the effects of different drying methods on quality of Chinese wolfberry powder. [Methods] The physical properties and nutritional contents of Chinese wolfberry powder prepared by hot air drying,vacuum freeze drying,microwave vacuum drying,drum drying and spray drying were measured and analyzed to explore the effects of different drying methods on quality characteristics of Chinese wolfberry powder. [Results] The total sugar content and reducing sugar contents of product: vacuum freeze drying > spray drying > microwave vacuum drying > drum drying > hot air drying; carotene and total flavonoid contents: vacuum freeze drying > microwave vacuum drying > spray drying > drum drying > hot air drying; after drying process,the dietary fiber content of product obtained by drum drying and spray drying was relatively higher,while the Vc content of Chinese wolfberry powder prepared by vacuum freeze drying was the highest. a~*value( redness to greenness) of color: vacuum freeze drying > microwave vacuum drying > spray drying > drum drying > hot air drying; the Chinese wolfberry powder prepared by vacuum freeze drying had the best solubility,hygroscopicity and rehydration ratio. [Conclusions] Microwave vacuum drying was the best method to prepare Chinese wolfberry powder,in order to keep its quality and consider the cost of production.

    2017年04期 v.8 27-31页 [查看摘要][在线阅读][下载 1835K]
  • Thin Layer Chromatography Screening for Glycyrrhiza uralensis

    Lijing CAO;Lihua YUAN;Kaiyan LIAO;

    [Objectives] To establish a simple and rapid,specific and reproducible thin layer chromatography( TLC) method,and accordingly establish a thin layer identification method for quality control of Glycyrrhiza uralensis. [Methods] According to the physical and chemical properties,components of Glycyrrhiza uralensis were identified by thin layer chromatography. According to Veterinary Pharmacopoeia of the People's Republic of China 2010( Volume Ⅱ) and related literature report,the thin layer chromatography identification method was studied and improved for Glycyrrhiza uralensis.[Results] When the preparation method for test sample solution was 6g Glycyrrhiza uralensis added with 30 mL saturated n-butanol,ultrasonic processing 20 min,filtered,filtrate dried,added 3 mL methanol and obtained Glycyrrhiza uralensis test sample solution. Then,chloroform-methanol-formic acid-ethyl acetate( 10∶ 3∶ 2∶ 3) as developing solvent,clear spot with high degree of separation and reproducibility could be obtained,and can be used for thin layer chromatography identification of ammonium glycyrrhetate in Glycyrrhiza uralensis.[Conclusions] The modified method omitted the step of ether treatment of samples,and directly adopted saturated n-butanol to extract components. It does not influence the detection of chromatographic spots,and solves the issue of sample gelatinizing sticking and filtration,simplifies the test process,and reduces the harm to human body.

    2017年04期 v.8 32-36页 [查看摘要][在线阅读][下载 1989K]

Pharmacological and Toxicological Analyses

  • Immediate-release Mechanism of Dehydrotrametenolic Acid and Dehydroeburicoic Acid in Poriae Cutis Total Triterpenoids Tablets Based on Liquid-solid Compact Technique

    Xuesheng YAN;Dandan SUN;Yanwei GUO;Xingang XU;Beibei YU;

    [Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotrametenolic acid and dehydroeburicoic acid as indicators,differences in dissolution were compared between liquid-solid compressed tablets and crude drug of total triterpenoids in Poriae Cutis;crude drug,powder of liquid-solid compressed tablets and excipients of liquid-solid compressed tablets were characterized by differential scanning calorimetry( DSC).[Results]Liquid-solid compact technique could significantly improve dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid. The total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets was 92%,t50( time for 50% total dissolution rate) and t_D( time for 63. 2% total dissolution rate) were 11. 18 min and 22. 71 min; the total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in crude drug of total triterpenoids was 29%,and t50 and t_D were231. 06 min and 359. 23 min. DSC showed that there was no mutual interaction between excipients and total triterpenoids in Poriae Cutis; on the DSC curve for powder of liquid-solid compressed tablets,the absorption peak vanished completely,indicating that the drug exists in the amorphous form in the liquid-solid powder.[Conclusions] Liquid-solid compact technique can increase the dissolution rate of total triterpenoids in Poriae Cutis and allow rapid release of poorly water soluble drugs.

    2017年04期 v.8 37-39+47页 [查看摘要][在线阅读][下载 659K]
  • Pharmacodynamics Study in vitro and in vivo of Zijin Huadu Suppository Treating Cervicitis

    Shanshan GUO;Yingjie GAO;Lei BAO;Yahong JIN;Yanying LI;Xiaolan CUI;Wei XIAO;

    [Objectives] To observe the pharmaceutical effect of Zijin Huadu suppository on treatment of cervicitis induced by virus,bacteria and phenol in vivo and in vitro,and provide experimental basis for evaluating its therapeutic effects for cervicitis. [Methods]Real-time RT-PCR and CPE methods were used to observe the inhibition of Zijin Huadu suppository on virus,and turbidimetry was used to observe bacteriostatic action; HE pathological section was used to observe cervicitis of rat,and enzyme-linked immunosorbent assay was used to detect virus' s expression in cervical tissue.[Results]In cervicitis rat model infected by HPV16,Zijin Huadu suppositories at the doses of 1. 2,0. 6 and0. 3 g/( kg·d) had significant inhibitory effect on HPV16 expression in cervical tissues,and the doses of 1. 2 and 0. 6 g/( kg·d) had significantly inhibitory effect on pathological changes of cervical tissues. In cervicities rat models infected mixedly by Escherichia coli,Neisseria gonorrhoeae and Staphylococcus aureus,Zijin Huadu suppositories at the doses of 1. 2 and 0. 6 g/( kg·d) had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances,Zijin Huadu suppositories at the dose of1. 2 g/( kg·d) had significant inhibitory effect on vagina and cervix lesions in rats. In vitro,Zijin Huadu suppository showed obvious inhibitory effects on HSV-2,HPV16,Staphylococcus aureus,Staphylococcus albus,Staphylococcus epidermidis,group B Streptococcus,E. coli,Pseudomonas aeruginosa,Proteusbacillus vulgaris,Neisseria gonorrhoeae and Candida albicans.[Conclusions] Zijin Huadu suppository has obvious inhibitory effect in vitro on viruses and bacteria,and could obviously improve the phenol-induced cervicitis.

    2017年04期 v.8 40-47页 [查看摘要][在线阅读][下载 3923K]
  • Effect of Decanoic Acid on the Cognitive Function of AD Mice

    Zheng LI;Xiaocheng ZHU;Xiaoqun DUAN;

    [Objectives] To study the effect of PPARγ agonist DA on the cognitive function of AD mice as well as the mechanism.[Methods]50 Kunming male mice were randomly divided into normal control group,model group and decanoic acid administration group,the AD mice model was established by subcutaneous injection of D-galactose for 8 weeks,and the treatment group was administered orally with different doses of decanoic acid( low dose of 50 mg/kg,middle dose of 100 mg/kg,high dose of 200 mg/kg). After 8 weeks,the Morris water maze was used to detect the learning and memory capability of mice; HE staining was used to observe the morphological change of hippocampal cells in brain tissue; Western-blot and immunohistochemistry technique were used to detect the expression of PPARγ and Aβ42 protein in brain tissue; the ELISA method was used for the determination of TNF-α and i NOS level in serum. [Results]Morris water maze results showed that DA could significantly improve the learning and memory function in AD mice( P < 0. 05); HE staining showed that DA could significantly reduce degeneration in hippocampal cells; Western-blot and immunohistochemistry results showed that DA could promote the expression of PPARγ and reduce the expression of Aβ42 in brain tissue( P < 0. 05); ELISA results showed that the TNF-α,i NOS levels decreased in AD mice serum after DA treatment( P < 0. 05). [Conclusions] DA could significantly improve the cognitive function of AD mice,improve the degeneration of hippocampal cells in brain tissue,and decrease the expression of Aβ42 in brain tissue. The mechanism might be related to activation of PPARγ protein and down-regulation of expression of relevant inflammatory factors.

    2017年04期 v.8 48-51页 [查看摘要][在线阅读][下载 1738K]
  • Study on Cisplatin Aggravating DNA Damage and Causing a High Apoptosis Rate on Breast Cancer MCF-7 Cells

    Liming YUAN;Nan MA;Jiaohuan CAO;Yi WEN;Xiangguang LIU;Xianxian ZHOU;Shuwen KUANG;Mengjie YANG;Wanxin OUYANG;Shijie JIA;Haibin WANG;Xiaojun TAO;Zhaojun ZENG;

    [Objectives] To investigate the mechanism of DNA damage of cisplatin( DDP),a broad spectrum anticancer drug on breast cancer MCF-7 cells,and to study the mechanism of apoptosis induced by DDP.[Methods]MCF-7 cells were treated by DDP( 0 mg/L,2 mg/L,4 mg/L,6 mg/L,6 mg/L,and 10 mg/L) for 48 hours. MTT assay was used to detect the inhibitory effect of DDP on MCF-7 cells and IC50 value was calculated. Western blot was adopted to detect the expression of γ-H2 AX,which was the marker of DNA double stranded breaks( DSBs) and ATM( sensory molecules of DSBs),the apoptotic signal transduction molecule cleaved caspase-3,and the proteins associated with apoptosis calpain.[Results]DDP inhibited MCF-7 cell activity in a concentration-dependent manner and IC50 was 7. 57 mg/L. In contrast to the control group( without DDP treatment),MCF-7 cells with DDP treatment expressed more γ-H2 AX,ATM,cleaved caspase-3 and calpain.[Conclusions] DDP could inhibit the activity of breast cancer MCF-7 cells. Its mechanisms may be associated with inhibition of MCF-7 cell apoptosis,induction of DNA double strand breaking and the expression of pro-apoptotic protein up-regulation.

    2017年04期 v.8 52-54+61页 [查看摘要][在线阅读][下载 679K]
  • On the Protective Effects of Aplysin on Ethanol-induced Oxidative Damage in Rat Primary Hepatocytes

    Ai SU;Hongyan ZHU;Hongwei XU;Ying LIU;Hui LIANG;

    [Objective] To investigate the protective effects of Aplysin on ethanol-induced oxidative damage in rat primary hepatocytes.[Methods]Rat primary hepatocytes were obtained via the portal vein collagenase IV in situ perfusion technique followed by a Percoll density gradient centrifuge; MTT test was used to determine the optimum dose of Aplysin and ethanol,and detect the cell vitality in primary hepatocytes; supernatants of primary hepatocytes were harvested to measure AST and LDH level,and the SOD,GSH-PX activities and MDA content in primary hepatocytes were observed; flow cytometry was used to detect the cell apoptosis rate; DNA damage in primary hepatocytes were detected by single-cell gel electrophoresis assay; The level of mitochondrial membrane potential in primary hepatocytes was tested by fluorogenic probe JC-1; the CYP2E1 activity in primary hepatocytes were detected by colorimetry; the proteins of CYP2E1 were detected by Western blotting. [Results]300 mmol/L dose of ethanol and 30 mg/L dose of Aplysin were the optimal dosages and were used in the subsequent experiments. Hepatocyte vitality was significantly increased in the Aplysin group relative to that in the ethanol group,and Aplysin inhibited the release of AST and LDH( P < 0. 05). For the Aplysin treatment group,the activities of hepatocyte SOD and GSH were significantly increased and MDA was markedly lowered as compared with those in the ethanol group( P < 0. 05). And Aplysin can alleviate hepatocyte apoptosis significantly,and hepatocyte DNA damage rate of II-III level and IV level were significantly lowered in the Aplysin treatment group as compared with those in the ethanol group,and Aplysin has evidently improved on alcohol induced mitochondria damage of hepatocyte. Primary hepatocytes activities and protein expression of CYP2E1 were markedly lowered in the Aplysin treatment group as compared with those in the ethanol group( P < 0. 05). [Conclusion] Aplysin has protective effects on liver oxidative damage induced by alcohol of primary cultured rat hepatocytes by blocking CYP2E1 activation,relieving oxidative stress,and sharpening the oxidation resistance ability.

    2017年04期 v.8 55-61页 [查看摘要][在线阅读][下载 2016K]
  • In Vitro Antioxidant Activity of Hot Water Extracts from 7 Different Sources of Ganoderma Lucidum

    Jian NIE;Shuilian YANG;Meihua MO;Zhihao HU;

    [Objectives] To study the in vitro antioxidant activity of hot water extracts from 7 different sources of Ganoderma lucidum mushroom.[Methods] The in vitro antioxidant activity of hot water extracts from 7 different sources of G. lucidum was studied from total reducing capacity,scavenging of hydroxyl free radical( ·OH),superoxide anion( O_2~(2-)·),and DPPH free radical.[Results]Under the same concentration,hot water extracts wild G. lucidum in Maoer Mountain showed the strongest reducing capacity with the absorbency being 2. 48,the highest scavenging capacity of hydroxyl free radical( · OH) with the scavenging rate being 99. 46%,and the strongest scavenging ability of DPPH free radical with its scavenging rate being 96. 62%. The ability of scavenging superoxide anion free radical( O_2~(2-)·) ranked the third.[Conclusions]The hot water extracts from Maoer Mountain wild G. lucidum showed the highest antioxidant ability in three aspects,indicating that Maoer Mountain wild G. lucidum has high development potential.

    2017年04期 v.8 62-65+71页 [查看摘要][在线阅读][下载 1107K]
  • Research Progress on Acorus tatarinowii Schott and Its Active Ingredients Preventing and Controlling Alzheimer's Disease

    Chengshu LU;

    It is hot point of research to prevent and control Alzheimer's disease( AD) by traditional Chinese medicine. Acorus tatarinowii Schott extract and its contained chemical compositions could play multiple pharmacological effects in central nervous system,in which the reports on main active parts and chemical compositions( such as volatile oil and β-asarone) from A. tatarinowii improving learning memory and preventing AD are the most in recent years. Based on referring to related literature at home and abroad,different pharmacodynamic actions of A. tatarinowii extract and main chemical compositions on AD and action mechanism are summarized.

    2017年04期 v.8 66-71页 [查看摘要][在线阅读][下载 313K]
  • An Ideal Raw-radish Therapy for Allergic Rhinitis

    Nianwei QIU;

    [Objectives] To explore a kind of simple and effective dietotherapy for allergic rhinitis( AR). [Methods]48 patients with AR were hospitalized and randomly assigned to raw-radish treatment group( 36 patients) or placebo group( 12 patients). Every patient took about 150 g raw radish( experimental group) or cooked radish( placebo group) one time or separately a day. [Results]By continuously taking raw radish for fifteen days,the total cure rate reached 80. 6% and total valid rate reached 94. 4%,with the valid rate 100% for persistent AR and 66. 7% for intermittent AR. But cooked radish( placebo) was invalid to AR. Presumably the wonder efficacy of raw radish may be related with isothiocyanates in radish. Since isothiocyanates are susceptible to hydrolysis by cooking,so radish must be eaten raw. [Conclusions] Raw radish therapy works wonders for AR,which may be more effective,safer,cheaper and simpler method for prevention and treatment of AR at present.

    2017年04期 v.8 72-74页 [查看摘要][在线阅读][下载 437K]

  • Medicinal Plant

    <正>Indexed by Chemical Abstracts(CA),AGRIS Database,CAB International(CABI),China National Knowledge Infrastructure(CNKI),Naresuan University Library,Indian Fund for Agricultural Development,Royal Tropical Institute,Library of Congress,Produest So far,a total of more than 3000 users have subscribed to Medicinal Plant,distributed in 6 countries and regions.The high-end users are as follows:

    2017年04期 v.8 2+75页 [查看摘要][在线阅读][下载 2860K]
  • AGRIS

    <正>AGRIS(The Agricultural Sciences and Technology)is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO.It embodies continued publications,documents,corpuses,books,technology reports,patents,maps,meeting papers,and other literatures,which are collected by 135 countries and regions,146

    2017年04期 v.8 14页 [查看摘要][在线阅读][下载 96K]
  • 下载本期数据