Review

  • Sampling Investigation of the Price Distribution of Pharmaceutical Preparations in Medical Institutions of Sichuan Province

    Sha SHU;Yu MAO;Zuojun XU;Congcong YU;

    [Objectives] To survey the current using situation and price distribution of pharmaceutical preparations in medical institutions of Sichuan Province,to provide basis for pricing the pharmaceutical preparations in medical institutions. [Methods] From June to September2016,15 sample hospitals were selected from Ⅱ-B class,Ⅱ-A class,Ⅲ-B class and Ⅲ-A class hospitals,and questionnaire survey was adopted to survey the current situation of pharmaceutical preparations in medical institutions,and 24 effective questionnaires were collected.SPASS17. 0 software was used to analyze the data of the questionnaires. [Results]The pharmaceutical preparations in medical institutions between 10 and 15 yuan accounted for 35%,which was the biggest percentage. The second was between 5 and 10 yuan,which accounted for 25%. [Conclusions] Most of the pharmaceutical preparations in medical institutions of Sichuan Province are in the low price,and floating rate price adjustment mechanism is suggested.

    2017年02期 v.8 1-3页 [查看摘要][在线阅读][下载 322K]

Chemical Composition of Natural Medicine

  • Thin Layer Chromatography and Methodological Investigation of Matrine in Zhukuqin Granule

    Wei WEI;Wenjie ZHONG;Juan LIU;Rongbin LING;

    [Objectives] To establish a sensitive and simple thin layer chromatography method of matrine in Zhukuqin granule. [Methods]With Zhukuqin granule as control medicinal materials,matrine as reference substance,we chose orthogonal test method,systematically investigated various factors affecting the thin layer chromatography,and selected the best thin layer chromatography conditions. [Results] We screened out the best thin layer chromatography of matrine in the Zhukuqin granule with chloroform as extraction solvent,solvent extraction and ultrasonic extraction as extraction method,toluene-acetone-ethyl acetate-ammonia solution( 2:3:4:0. 5) as developing agent,improvement of bismuth potassium iodide solution as color developing agent. [Conclusions] The separating degree of this method was high,it had good repeatability,and it could be used to control quality of Zhukuqin granule.

    2017年02期 v.8 4-7页 [查看摘要][在线阅读][下载 930K]
  • Separation and Purification of Acetophenones from Cynanchum bengei Decne Root Bark by Combination of Silica Gel and High-speed Counter-current Chromatography

    Huaizhi LI;Lingchuan XU;Xiao WANG;Qian LIU;Jia LI;Peng YANG;Bingtian YANG;

    [Objectives] To develop a method for separation and purification of acetophenones from Cynanchum bengei Decne root bark by combination of silica gel and high-speed counter-current chromatography( HSCCC). [Methods]The crude extract of Cynanchum bengei Decne root bark was separated by silica gel column chromatography,and parts A and B containing acetophenones were obtained. Then,parts A and B were separated by HSCCC with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water( 4∶ 6∶ 4. 5∶ 5. 5 and4∶ 6 ∶ 3 ∶ 7, V/V), respectively. [Results] From 260 mg of part A, four compounds with p-dihydroxybenzene 3. 9 mg(Ⅰ),4-hydroxyacetophenone 17. 1 mg( Ⅱ),2,5-di-hydroxyacetophenone 13. 3 mg(Ⅲ) and 2,4-dihydroxyaceto-phenone 21. 0 mg(Ⅳ) were obtained. And from 300 mg of part B,136 mg of Radix Cynanchi Bungei benzophenone(Ⅴ) was obtained. The purity of compounds determined by HPLC was 97. 0%,96. 6%,99. 2%,99. 7%,99. 5%,respectively. [Conclusions] The established method is simple and efficient. It can be used for separation of acetophenones from Cynanchum bengei Decne root bark and has better practical value,which could provide a reference basis for development and utilization of Cynanchum bengei Decne root bark.

    2017年02期 v.8 8-11页 [查看摘要][在线阅读][下载 1040K]
  • Extraction Technology of Total Flavonoids from Pueraria lobata (Willd.) Ohwi and Content Determination of Pueraria lobata (Willd.) Ohwi at Different Altitudes

    Chunlin LIU;Yingchen LI;Bochao LI;Yuan LIU;

    [Objectives]To optimize the extraction technology of total flavonoids from Pueraria lobata( Willd.) Ohwi. [Methods]The ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in Pueraria lobata( Willd.) Ohwi. With the puerarin as index,the reflux extraction and single factor test were employed to investigate the effects of temperature,time,ethanol concentration and solid-liquid ratio on the content of total flavonoids in Pueraria lobata( Willd.) Ohwi,respectively. Under the optimal extraction technology,the content of total flavonoids in Pueraria lobata( Willd.) Ohwi at different altitudes was determined.[Results] The optimum extraction process was as follows: 70%ethanol; solid-liquid ratio of 1∶ 30; 1 h reflux extraction. Under these conditions,the extraction rate of flavonoids in Pueraria lobata( Willd.) Ohwi was 11. 48%,the total flavonoids content of different kudzu parts was in the order of roots > stems > leaves,and the total flavonoids content of the sample at about an altitude of 1000 m was significantly higher than at the altitudes of 1400 m and 1700 m.[Conclusions]It was suggested that the Pueraria lobata( Willd.) Ohwi should not be cultivated as medicinal plant in too high mountains,and the stems and leaves of Pueraria lobata( Willd.) Ohwi could be used as raw materials for extracting total flavonoids.

    2017年02期 v.8 12-14+18页 [查看摘要][在线阅读][下载 855K]

Processing of Traditional Chinese Medicine and Quality Control

  • Optimization of Extraction Process of Total Flavonoids from Akebia trifoliata (Thunb.) Koidz. by Central Composite Design-Response Surface Methodology

    Houkang CAO;Boting XI;Simao HUANG;Xiaohua PANG;Kefeng ZHANG;

    [Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.

    2017年02期 v.8 15-18页 [查看摘要][在线阅读][下载 1279K]
  • Comparison of Extraction Methods for Total Alkaloids from Abrus Cantoniensis Hance

    Mengyao LIN;Qiumeng WEI;Xiaoxia HUANG;Jiaming WANG;Suoyi HUANG;Luyao WANG;Yan LIU;Gongxiao MO;Xunjie LIANG;Xiaoting MA;

    [Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.

    2017年02期 v.8 19-21页 [查看摘要][在线阅读][下载 278K]
  • Study on the Quality of Traditional Chinese Veterinary Medicine and Discussion on the Problems

    Zhiqiang YAN;Shaoqin ZHAI;Maixun ZHU;Hua ZHENG;Guowen CAO;

    In this paper,the study on the quality of traditional Chinese veterinary medicine and its existing problems are discussed,which mainly include the research techniques and research thoughts of traditional Chinese veterinary medicine,and the problems in its research progress,at the same time,the causes for the quality problems of traditional Chinese veterinary medicine are analyzed,and the solutions for the problems are put forward. The quality research of traditional Chinese veterinary medicine should refer to traditional Chinese medicine,then inherit,carry forward and innovate it based on the modern research. Solving the problems in the development of traditional Chinese veterinary medicine and putting forward the thinkings conforming to the development of traditional Chinese veterinary medicine using advanced instruments and devices as well as science and technology are urgent affairs.

    2017年02期 v.8 22-24页 [查看摘要][在线阅读][下载 113K]
  • Orthogonal Test Optimization of Extraction Technology of Total Fla-vonoids from Fruits and Leaves of Prunus mume Sieb. et Zucc. and Its in Vitro Anti-oxidation Activity

    Xue ZHAI;Qian JIN;Haiyan XIANG;Fenglin WANG;Yuan LIU;

    [Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.

    2017年02期 v.8 25-31+36页 [查看摘要][在线阅读][下载 3328K]

Pharmacological and Toxicological Analyses

  • Pharmacognosy Identification of Yi Nationality Crude Herb "Te-zhi-pa"

    Yun TENG;Shan LI;Kaishun WANG;Pei QUN;Yuan LIU;

    [Objcetives]To study on the original plant,medicinal materials and microscopic identification of Yi Nationality crude herb Te-zhipa of Ageratum conyzoides L.,and provide experimental data for identification and application. [Methods] The original plant identification,character identification,microscopic structure identification,powder identification were adopted in theresearch.[Results] The characteristics of Yi Nationality crude herb Te-zhi-pa were obvious in the original plant,crude herband microscopic characteristics. [Conclusions]The result was provided some reference for the formulation of quality standards and original plant identification for Yi Nationality crude herb " Te-zhi-pa",and provided the basis for the further research.

    2017年02期 v.8 32-36页 [查看摘要][在线阅读][下载 6978K]
  • Immunoregulatory Effects of Nuhuang Fuzheng Granules on Mice with Cytoxan-induced Immunosuppression

    Chuncheng YU;Yifan ZHANG;Shaoqin ZHAI;Hua ZHENG;Maixun ZHU;

    [Objectives]To study immunoregulatory effects of Nuhuang Fuzheng Granules on mice with Cytoxan-induced immunosuppression.[Methods]Kunming variety mice were randomly divided into the control group,model group,positive medicine group,and low,medium,and high dosage groups of Nuhuang Fuzheng Granules. Except the control group,the rest groups of mice were injected with Cytoxan at60 mg/kg to reproduce the immunosuppressive mice model,and were fed through stomach at 0. 5g/kg,1. 0 g/kg and 2. 0 g/kg Nuhuang Fuzheng Granules for seven days( twice a day); the positive control group was fed with Astragalus Polysacharin( 500 mg/L). The carbon clearance index,immune organ index,serum hemolysin were determined. IL-2,IFN-γ,and IL-4 levels in mouse serum were measured by ELISA. [Results] Nuhuang Fuzheng Granules could significantly incease the carbon granular expurgation index of mice with Cytoxan-induced immunosuppression,and the 2. 0 g/kg and 1. 0 g/kg dosage groups had better effect than 0. 5 g/kg dosage group( P < 0. 05); the spleen index of mice with Cytoxan-induced immunosuppression increased significantly and there was dose-effect relationship in a certain range of concentration; the thymus index and serum hemolysin HC_(50) were significantly higher than 0. 5 g/kg dosage group( P < 0. 05); IL-4 and IFN-γ in the serum was significantly higher than the model group( P < 0. 01),the increase of IL-2 was not significantly( P > 0. 05). [Conclusions] Nuhuang Fuzheng Granules can promote growth of immune organs and secretion of cytokines through improving the carbon granular expurgation ability of mice with Cytoxan-induced immunosuppression.

    2017年02期 v.8 37-39+44页 [查看摘要][在线阅读][下载 134K]
  • Pharmacognosy Identification of Yi Crude Herb Pholidota chinensis Lindl.

    Qian JIN;Haiyan XIANG;Pei QUN;Ying LI;Yuan LIU;

    [Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identification,character identification,powder identification and paraffin section identification were adopted in the study. [Results]The characteristics of Yi Crude herb of Pholidota chinensis Lindl. were obvious in the original plant,Crude herb and microscopic characteristics. [Conclusions]This paper provided reference for developing quality standards of Yi Crude herb Pholidota chinensis Lindl. and original plant identification,and provided the basis for further research.

    2017年02期 v.8 40-44页 [查看摘要][在线阅读][下载 8870K]
  • Preparation and Evaluations of Ibuprofen Thermosensitive in situ Gel

    Miao YU;Shangcong HAN;Jialing DAI;Jiao LIU;Chen WEI;Yitong LI;Lutao JIANG;Yong SUN;

    [Objectives] To prepare Ibuprofen-β-CD for thermosensitive in situ gel administration to achieve a slow drug release. [Methods]By saturation solution process,the best inclusion technology was screened out with orthogonal design. [Results]The prescription of in situ gel contained ibuprofen-β-CD complex,poloxamer P407,P188,and HPMCK4M( w/v 10%,18%,3%,and 0. 5%). The gelation temperature of ibuprofen gel was 34. 6 ± 0. 3℃. The viscosity of ibuprofen gel increased significantly above 35℃ and showed semi-solid attribute. [Conclusions] The release of ibuprofen from in situ gel was slow and stable in 96 h,demonstrated a proximate zero order release between 24 h and 96 h.

    2017年02期 v.8 45-47+50页 [查看摘要][在线阅读][下载 477K]

Clinical Application

  • Treatment of Peripheral T-cell Lymphoma by Chidamide and Literature Analysis

    Xuerong NIE;Liangming ZHANG;Dongmei GENG;Minghua SUI;Xuan ZHAO;

    [Objectives] To analyze the dosage,curative effect,and adverse effect of Chidamide in treating peripheral T-cell lymphoma( PTCL). [Methods]A case of treating peripheral T-cell lymphoma by Chidamide was reported,including the treatment process,dosage,curative effect,and adverse effect. Literature review was made and searched in Wanfang Digital Database,China National Knowledge Infrastructure( CNKI),and Pubmed database,using the key word chidamide in Chinese and English separately. Disease and case number of patients,Chidamide observation indicator,curative effect,and adverse effects were recorded in detail. The search was carried out as of September,2016. [Results] It searched 3 articles related to clinical application and 111 cases of patients. [Conclusions] Chidamide has excellent curative effect in treating peripheral T-cell lymphoma and is suitable for clinical application.

    2017年02期 v.8 48-50页 [查看摘要][在线阅读][下载 110K]
  • Clinical Study on the Relationship between Fixation of Prosthesis and Health of Surrounding Tissues

    Lei LI;Yanzheng SUN;Shengteng SUN;Xinxin SUI;Junnan XIA;Yi LI;Jianjun YANG;

    [Objectives] To explore the effect of mouth outside bonding screw fixation and inside bonding fixation in implanting the prosthesis( crown or bridge) on the health of surrounding tissues,to provide a certain reference for the selection of fixation methods for clinically implanting the prosthesis. [Methods]160 cases of patients needing implanting the prosthesis due to dental arch deficiency were selected. They had excellent alveolar bone. They did not smoke,had no diabetes,and their bone tissue healing was at the early stage. They were randomly divided into A and B groups,80 cases in each group. Group A: the mouth inside and abutment of prosthesis was bonded to a single abutment crown or a single abutment bridge; the bonder on the surface of prosthesis was completely removed and then moved into the implant,and fixed with central retaining screw,this group was called " mouth outside bonding group". Group B: the abutment was located in the implant and fixed using central retaining screw,then the prosthesis( crown or bridge) was bonded to the abutment,the bonder on the surface of prosthesis was removed,and this group was called mouth " mouth inside bonding group". The health condition was compared between two fixing methods for the surrounding tissues of the implant in 24-36 months after restoration. [Results] Group A: the surrounding tissues of the implants for 126 teeth of 80 patients were healthy without occurrence of peripheral inflammation; Group B: in the implants of 112 teeth of 80 patients,7 teeth suffered the peripheral inflammation. Dental implant examination found that there was residue of bonder in the bonding of prosthesis and abutment,3 teeth exfoliated,and 4 teeth were restored after treatment. Through the X2 test,there was significant difference between Group A and Group B( P < 0. 05). [Conclusions] Implant-supported denture often adopts the mouth inside bonding. Since the bonder can not be removed completely,its residue will remain in gingival trough and stimulate the surrounding tissues of the implant and bring about the inflammatory reaction,and even lead to exfoliation of the implant; the mouth inside and abutment of prosthesis was bonded to a single abutment crown or a single abutment bridge,the bonder on the surface of prosthesis was completely removed and then moved into the implant,and fixed with central retaining screw. This method is an effective method for preventing the peripheral inflammation and increasing the success rate of dental implant.

    2017年02期 v.8 51-53+56页 [查看摘要][在线阅读][下载 1429K]
  • Expression of Med 19 Protein in Tongue Cancer and Its Significance

    Xuyang LIU;Lingling LI;Shengzhi WANG;

    [Objectives] To study the expression of Med 19 protein in tongue cancer and its significance. [Methods] Immunohistochemical method was used to detect the expression of Med 19 protein in tongue cancer tissue and paracancerous tissue. [Results]The expression of Med19 protein in tongue cancer tissue was higher than paracancerous tissue and normal tissue. The expression of Med 19 protein was related to the tumor lymph node metastasis. However,there was no significant difference in sex,age,Tumor Node Metastasis( TNM),and differentiation degree. [Conclusions] Med 19 protein participates in incidence and development of tongue cancer and may become diagnostic indicator and therapeutic target for tongue cancer.

    2017年02期 v.8 54-56页 [查看摘要][在线阅读][下载 654K]

  • Medicinal Plant

    <正>Indexed by Chemical Abstracts(CA),AGRIS Database,CAB International(CABI),China National Knowledge Infrastructure(CNKI),Naresuan University Library,Indian Fund for Agricultural Development,Royal Tropical Institute,Library of Congress,ProQuest So far,a total of more than 3000 users have subscribed to Medicinal Plant,distributed in 6 countries and regions.The high-end users are as follows:

    2017年02期 v.8 2页 [查看摘要][在线阅读][下载 958K]
  • AGRIS

    <正>AGRIS(The Agricultural Sciences and Technology)is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO.It embodies continued publications,documents,corpuses,books,technology reports,patents,maps,meeting papers,and other literatures,which are collected by 135 countries and regions,146

    2017年02期 v.8 3页 [查看摘要][在线阅读][下载 75K]
  • Chemical Abstracts

    <正>Chemical Abstracts(CA),a reputable international search tool,which involves the widest disciplinary field,collects the most literature types,offers the most searching ways,and has the most prodigious volumes,is edited and published by CAS-Chemical Abstracts in

    2017年02期 v.8 50页 [查看摘要][在线阅读][下载 83K]
  • Submit your manuscript today! MEDICINAL PLANT

    <正>Sponsored by the Wuchu(USA-CHINA)Science and Culture Media Corporation Medicinal Plant(ISSN 2152-3924)is an academic journal sponsored by USA-China Science&Culture Media Corporation in January 2010,published monthly in the general format of US Sci-tech journal.The subject areas covered by the journal include crude drug processing,chemical components analysis,medical mechanism study,wild

    2017年02期 v.8 57页 [查看摘要][在线阅读][下载 736K]
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