Review

  • Discussions about Modern Standardized Production of Genuine Traditional Chinese Herbal Medicines

    Junshe SUN;Zhian ZHENG;Xiuqing ZHANG;Yue YANG;

    In recent years,with the vigorous development of the Chinese herbal medicine market in China,the government and various circles of society pay close attention to the safety of Chinese herbal medicine production. The cultivation and production of traditional Chinese herbal medicine already could not satisfy the current market demands. This paper summarized the forming,changing and the influencing factors of Chinese herbal medicines,and organized the research for the formation of traditional Chinese herbal medicines by modern scholars in China. It reached the following conclusion: the production of modern Chinese herbal medicines should ensure its unique properties,and the more important is to guarantee the quality and safety. With the continuous improvement of science and technology innovation ability and the appraisal method of Chinese medicines,the better management system has been applied in the process of planting. This paper summarized the six attributes of modern production of Chinese herbal medicines,to ensure genuine,safe and standardized production of traditional Chinese medicines.

    2017年01期 v.8 1-6页 [查看摘要][在线阅读][下载 352K]

Chemical Composition of Natural Medicine

  • Contrastive Study on Content and Anti-oxidation Activity of Total Saponins from Cultivated and Wild Paris Plants in Southwest China

    Mengjie YU;Haiyan XIANG;Shaoshan ZHANG;Zhongjie HUANG;Yuan LIU;

    [Objectives]To study content of total saponins from cultivated and wild Paris herbs in Southwest China,evaluate its in vitro antioxidation activity,and explore the correlation between quality of Paris herbs and production place,variety,cultivated and wild varieties.[Methods]Colorimetric method of perchloric acid reaction was used,and content of total saponins from Paris herbs was determined by ultraviolet spectrophotometer at 406 nm. [Results]The lowest content of total saponins from cultivated and wild Paris herbs in Southwest China was0. 55%,while the maximum was 12. 24%,with obvious difference. Total saponins from Paris herbs had stronger clearance ability on free radical DPPH and stronger reduction ability on Fe~(3+),and the results showed good dose-effect relationship with concentration. Among them,S6 had the highest clearance ability on DPPH,which reached 89. 66%. In the determination of reduction ability,the absorbance of S16 reached3. 643 and was the highest,with very good reducing property. Content and anti-oxidation capacity of total saponins from Paris herbs in Southwest China did not have obvious correlation with production place,altitude and cultivation but was related to variety. [Conclusions]The quality of Paris herbs in Southwest China was unstable,and their anti-oxidation abilities were also different,and cultivation could not guarantee high quality. It should normalize cultivation,and develop planting industry of Paris herbs,which could guarantee stable supply of Paris herbs and stability and controllability of quality.

    2017年01期 v.8 7-11页 [查看摘要][在线阅读][下载 504K]
  • Detection of Berberine Hydrochloride in Jin Weng Zhili Particle Using TLC and HPLC

    Li LIU;Lifang OUYANG;Dengfang CHENG;Juan LIU;Xuehong HU;Yuan LI;

    [Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography and high performance liquid chromatography were used respectively for qualitative and quantitative identification of berberine hydrochloride contained in Jin Weng Zhili Particle by improving the conditions of extraction and purification. [Results]After optimizing TLC conditions of Jin Weng Zhili Particle,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,and precision was high. Under the conditions of mobile phase constituted by acetonitrile-water-glacial acetic acid-triethylamine( 28∶ 70∶ 1∶ 1),detection wavelength of 265 nm,flow rate of 1. 0 m L min~(-1)and column temperature of 35℃,the resolution of berberine hydrochloride was good; the injection volume in the range of 3. 15-50 μg· m L~(-1)had good linear relationship with the peak area( R~2= 0. 998); the average sample recovery rate(n=6) was 5. 672%,and RSD was 0. 864%; the content of berberine hydrochloride contained in Jin Weng Zhili Particle was 4. 023 mg. [Conclusions]The method was of good feasibility,reproducibility and specificity,and could be used for qualitative and quantitative detection of berberine hydrochloride contained in Jin Weng Zhili Particle.

    2017年01期 v.8 12-16页 [查看摘要][在线阅读][下载 905K]
  • Determination of the Content of Total Saponins in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita

    Zhongjie HUANG;Haiyan XIANG;Shaoshan ZHANG;Mengjie YU;Yuan LIU;Hao ZHANG;

    [Objectives]To determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S.kurita,fill the gap in the study of Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and lay a foundation for searching new medicinal resources of Paris L. [Methods]The perchloric acid colorimetric method and UV spectrophotometer were used to determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita at 406 nm. [Results]The lowest content of total saponins in Paris daliensis H. Li et V. G. Soukoup was 2. 637%,while the highest content reached 5. 474%; the lowest content of total saponins in Paris dulongensis was 1. 806%,while the highest content reached 3. 532%. Through the methodological examination,it was found that this method was stable and reliable. [Conclusions] The content of total saponins was high in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and it was suggested to further study and find the medicinal resources of Paris L.

    2017年01期 v.8 17-19页 [查看摘要][在线阅读][下载 125K]
  • Study on the Determination Method for the Content of Tannin in Sanguisorba officinalis L.

    Siwei CHEN;Qin SUN;Fang WANG;Xin YU;Dan ZHANG;

    [Objectives] To establish a method for the determination of tannin in Sanguisorba officinalis L.,and provide guarantee for further control over quality of semi-finished tannin products from Sanguisorba officinalis L. [Methods] With gallic acid as reference substance,the UV method was used to determine the content of tannin in Sanguisorba officinalis L.,and the methodological examination was done. [Results]With gallic acid as standard,when the concentration of tannin in Sanguisorba officinalis L. was 1. 02-10. 14 μg·m L~(-1),there was a good linear relationship with absorbance,the precision,reproducibility and recovery were in line with the requirements,and the absorbance value was stable within 40 min after coloration. [Conclusions] The method was simple,rapid and accurate in determining the content of tannin in Sanguisorba officinalis L.

    2017年01期 v.8 20-21页 [查看摘要][在线阅读][下载 253K]
  • Study on Colorimetric Method and Content Determination of Total Phenol in Homemade Plum Wine

    Yun TENG;Pei QUN;Xue ZHAI;Yuan LIU;

    [Objectives] To study on colorimetric method and content determination of total phenol in homemade plum wine. [Methods]The optimal determination condition of total polyphenols content in plum wine( commercially available and homemade) by Folin-Ciocalteu method was inspected,and commercially available and homemade plum wine was evaluated by the method. [Results]The optimal determination conditions of total polyphenols content: sample dose of 1. 0 m L,Folin-Ciocalteu reagent of 1 m L,4% Na_2CO_3 solution of 4. 0 m L,reaction temperature of 50 ℃,reaction time of 1. 5 h,and determination wavelength of 756 nm. Absorbance showed good linear relationship with total polyphenols content within the range of 17. 73-59. 12 μg/m L( y = 14. 878 x + 0. 0739,R~2= 0. 9998). Recovery rate of adding standard sample was between 98. 8% and 103. 5%,and relative standard deviation was 2. 0%( n = 5). [Conclusions]The method had high precision degree and good stability,which was suitable for measuring total polyphenols content in plum wine( commercially available and homemade).

    2017年01期 v.8 22-26页 [查看摘要][在线阅读][下载 518K]

Processing of Traditional Chinese Medicine and Quality Control

  • Optimization of Extraction Method of Triterpenoids from Artemisia selengnesis Turcz

    Cunbing WU;Junyan WU;Linshuang TIAN;Yong DOU;Jing DONG;

    [Objectives]The research aimed to optimize the extraction method of triterpenoids from A. selengnesis. [Methods]The ultrasonic extraction of triterpenoids in A. selengnesis was optimized by single factor analysis( including infusion time,ethanol concentration,ultrasonic power,extraction time,and liquid-solid ratio) and response surface method. [Results]The optimum extracting process of triterpenoids was as follows: infusion time of 65 min,ethanol concentration of 80%,ultrasonic power of 275 W,extraction time of 30 min,and liquid-solid ratio of 34 m L/g. Under the optimized condition,the extraction content of triterpenoids reached( 18. 26 ± 0. 10) mg/g. [Conclusions] The optimized extraction had a good modal on practice in developing and utilization.

    2017年01期 v.8 27-31+34页 [查看摘要][在线阅读][下载 1430K]
  • Processing Technology of Mongolian Artemisia frigida Willd. and Pharmacological Study on Processed Products

    Zhuna;Sarula;Nashengsang;

    [Objectives] To optimize the processing technology of Mongolian Artemisia frigida Willd. and observe the safety and effectiveness of processed products. [Methods] The orthogonal test was conducted with the content of chlorogenic acid( CGA) in processed products and quercetin as the main indicator and reference factors. The impact of frying temperature and time and medicinal material granularity were examined to determine the optimal process. The safety and effectiveness were evaluated through adopting acute toxicity test in mice,and the bleeding time was determined by tail docking experiments,the blood coagulation time was determined with orbital venous plexus,the total number of platelets was counted for the whole blood. [Results]When the product was flipped and fried at the temperature of 270 ℃ for 20 min,the best effect was achieved. From the variance analysis,it proved that the frying temperature and the time and granularity had no significant effect on the content of the two components monitored. After processing,Artemisia frigida Willd.,it can shorten the bleeding time of mice and significantly increase the platelet number. [Conclusions]Appropriate frying can improve the safety and hemostatic effect. Therefore,this study can provide reference for verifying the theory of use of traditional Mongolian Artemisia frigida Willd.

    2017年01期 v.8 32-34页 [查看摘要][在线阅读][下载 126K]
  • Optimization of Extraction Technology and Determination of Content of Total Phenols of Leaves in Acanthopanax giraldii Harms

    Wei ZHAO;Yijun CHEN;Chunlin LIU;Yuan LIU;

    [Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.

    2017年01期 v.8 35-37+42页 [查看摘要][在线阅读][下载 292K]
  • Advances in Researches of Extraction Processes of Total Saponins of Pnanx Notoginseng

    Huaneng PAN;Suoyi HUANG;Qiaorong YU;Wende LU;Mengyao LIN;

    Traditional extraction methods of total saponins of Panax notoginseng include cold soaking method,water decoction method,alcohol reflux method,percolation method,macroporous resin adsorption method,and accelerated solvent extraction( ASE) method. Modern extraction methods include ultrasonic extraction,microwave assisted extraction,supercritical CO_2 extraction,microbial fermentation assisted extraction,neural network model optimized extraction method,and multi-stage countercurrent extraction method. This paper discussed principles of these methods and compared their advantages and disadvantages.

    2017年01期 v.8 38-42页 [查看摘要][在线阅读][下载 165K]

Pharmacological and Toxicological Analyses

  • Toxicity Experiment of Polysaccharides from Dicliptera chinensis( L. ) Juss.

    Kefeng ZHANG;Zhimin JIANG;

    [Objectives] To study the acute toxicity,mutation,and feeding experiment of polysaccharides from Dicliptera chinensis( L.)Juss.,and assess its safety. [Methods]In accordance with Procedures for toxicological Assessment on Food Safety,acute toxicity to mice,micronucleus experiment for bone marrow cell in mice,sperm shape abnormality test in mice,and Ames experiment were carried out,and 30 d feeding experiment was detected for rats. [Results] when the maximum tolerance dose( MTD) of mice was higher than 20 g/kg,at polysaccharides from Dicliptera chinensis( L.) Juss. ≤10 g/kg,there was no statistical difference between the control group and mouse bone marrow cell micronucleus and sperm shape abnormality test group; Ames experiment indicated that the number of revertant colonies of different dose groups of polysaccharides from Dicliptera chinensis( L.) Juss. with and without S9 did not exceed two times of the blank control group; rat 30 d feeding experiment indicated that when polysaccharides from Dicliptera chinensis( L.) Juss. ≤ 3. 2 g/kg,rats grew and developed well,and there was no statistical difference with the control group in blood,multiple organ biochemical indicators and liver tissue. [Conclusions]Polysaccharides from Dicliptera chinensis( L.) Juss. are safe and non-toxic chemical compounds and can be used as health products or drugs.

    2017年01期 v.8 43-44+47页 [查看摘要][在线阅读][下载 1343K]
  • A Study on Hypolipidemic Effect of Hawthorn Flavonoids Extract

    Ming ZHANG;

    [Objectives] To research the hypolipidemic effect of hawthorn flavonoids extract. [Methods] The hawthorn flavonoids were extracted by percolation method. The SD rats were used for the experiment,and after the formation of high blood lipids in rats,the low,middle and high doses of hawthorn flavonoids extracts were used for intragastric administration. After 30 d,the changes of blood lipid in rats were detected. [Results] Hawthorn flavonoids significantly reduced total cholesterol and LDL,and adjusted triglycerides and HDL in the blood of SD rats. [Conclusions] Hawthorn flavonoids extract had significant hypolipidemic effect.

    2017年01期 v.8 45-47页 [查看摘要][在线阅读][下载 123K]
  • The Protective Effect of Arabinose + Mannose on Mice with Acute Liver Injury Induced by CCl_4 and Its Mechanism

    Simao HUANG;Ya GAO;Houkang CAO;Kefeng ZHANG;

    [Objectives] Taking mice with acute liver injury induced by CCl_4 as the model,the effect of arabinose + mannose( w/w = 1∶ 1) on mice with acute liver injury induced by CCl_4 was studied. [Methods]60 experimental mice were selected and then randomly divided into normal control,model group and positive group( bifendate 120 mg/kg),high-dose arabinose + mannose group( 800 mg/kg),middle-dose arabinose + mannose group( 400 mg/kg) and low-dose arabinose + mannose group( 200 mg/kg),each group had 10 mice,which were fed adaptively for 1 week. Except normal control group and model group,each treatment group was given medicine by gavage once a day and lasted for7 days according to the dosage of 20 ml/kg. After the last drug,except normal control group,the mice of other groups were injected 10 ml/kg0. 12% CC14 peanut oil through enterocoelia,thereby establishing acute liver injury model. The mice were fasted but not water for 24 h,after that,blood was sampled from mice eyes,then dissected rapidly. The activities of ALT and AST in the serum were determined,the expression levels of TNF-α,IL-1β and IL-6 from the hepatic tissues were detected by enzyme-linked immunosorbent assay( ELISA); then after HE dye,the changes of liver histopathology were observed. [Results]Compared with CCl_4 model,the activities of ALT and AST from the serum of mice from high-dose and middle dose groups decreased significantly( P < 0. 01); the contents of TNF-α,IL-1β and IL-6 from the hepatic tissues of mice decreased significantly( P < 0. 01); the pathological section showed that the liver injury of mice from the combined drug groups showed alleviating trend to varying degrees,in which the liver injury of mice from the high-dose group was the best. [Conclusions] Arabinose + mannose has an obvious protective effect on mice with acute liver injury induced by CCl_4,and its mechanism may relate to anti-inflammatory.

    2017年01期 v.8 48-50页 [查看摘要][在线阅读][下载 1098K]

Biotechnology

  • Analysis on Composition and Diversity of Endophytes in Moringa oleifera

    Song JIN;Zebin CHEN;Tiyuan XIA;Yuchuan LI;Feng ZHAO;Zhen REN;

    [Objectives] To explore the diversity of endophytes in Moringa oleifera,and provide a scientific basis for better protection,development and use of Moringa oleifera resources. [Methods] With Moringa oleifera stems and leaves as materials,the Illumina Mi Seq highthroughput sequencing technology was used to study the endophyte diversity. [Results] A total of 199 OTUs were measured in the samples,belonging to 12 phyla such as Proteobacteria,Actinobacteria,Acidobacteria,Gemmatimonadetes,Bacteroidetes,Chloroflexi,DeinococcusThermus,Verrucomicrobia,Candidatus Saccharibacteria and Cyanobacteria. [Conclusions]Gemmatimonas is the dominant population of endophyte in Moringa oleifera.

    2017年01期 v.8 51-53+56页 [查看摘要][在线阅读][下载 1381K]

Other

  • Characteristics and Clinical Application of Prescriptions in Woman Section of Synopsis of Prescriptions of the Golden Chamber

    Qiankuan LI;Fei GAO;

    The three chapters in Woman Section of Synopsis of Prescriptions of the Golden Chamber are the earliest medical articles in the diagnosis and treatment of gynecological diseases. They have already had the embryonic form of traditional Chinese gynecology,and laid a certain foundation for the future development of gynecology. There are 35 prescriptions in the Woman Section of Synopsis of Prescriptions of the Golden Chamber,using 66 kinds of traditional Chinese medicines and discussing more than 20 kinds of gynecological diseases. There are complete theory,methods,formulas and medicinals,and internal and external therapies. In curing gynecological diseases,the prescriptions are highly characteristic and are worth discussion and learning.

    2017年01期 v.8 54-56页 [查看摘要][在线阅读][下载 127K]

  • Medicinal Plant

    <正>Indexed by Chemical Abstracts(CA),AGRIS Database,CAB International(CABI),China National Knowledge Infrastructure(CNKI),Naresuan University Library,Indian Fund for Agricultural Development,Royal Tropical Institute,Library of Congress,ProQuest So far,a total of more than 3000 users have subscribed to Medicinal Plant,distributed in 6 countries and regions.The high-end users are as follows:

    2017年01期 v.8 2页 [查看摘要][在线阅读][下载 1167K]
  • AGRIS

    <正>AGRIS(The Agricultural Sciences and Technology)is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO.It embodies continued publications,documents,corpuses,books,technology reports,patents,maps,meeting papers,and other literatures,which are collected by 135 countries and regions,146branches of AGRIS,and 22 international organizations.Its content comprises all the agricultural fields in the world,as well as forestry,economy,aquatic sciences,fishery,human dietetics,and so on.This database is an international intelligence communication system main-

    2017年01期 v.8 16页 [查看摘要][在线阅读][下载 94K]
  • Chemical Abstracts

    <正>Chemical Abstracts(CA),a reputable international search tool,which involves the widest disciplinary field,collects the most literature types,offers the most searching ways,and has the most prodigious volumes,is edited and published by CAS-Chemical Abstracts in the USA.CA has reported more than 10 000 kinds of technology journals,technology reports,meeting papers,academic dissertations,data chronicles,technique reports,new books,and audiovisuals,which are published by 150 countries worldwide in 56 langua-

    2017年01期 v.8 50页 [查看摘要][在线阅读][下载 97K]
  • Submit your manuscript today! MEDICINAL PLANT

    <正>Sponsored by the Wuchu(USA-CHINA)Science and Culture Media Corporation Medicinal Plant(ISSN 2152-3924)is an academic journal sponsored by USA-China Science&Culture Media Corporation in January 2010,published monthly in the general format of US Sci-tech journal.The subject areas covered by the journal include crude drug processing,chemical components analysis,medical mechanism study,wild

    2017年01期 v.8 57页 [查看摘要][在线阅读][下载 848K]
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