Chemical Composition of Natural Medicine

  • GC-MS Analysis on Volatile Oils from Underground Part of Origanum vulgare L.

    Huaqiang WU;Youer LIU;Manman QIN;Zhiyu ZHOU;Zhidong ZHAO;Fei HAN;Zhifu AI;

    [Objective] To study chemical composition of volatile oils from underground part of Origanum vulgare L. [Methods]Volatile oils were exacted by the steam distillation( SD) method,chemical components of volatile oils were analyzed and identified by gas chromatographymass spectrometry( GC-MS) method,and relative percentage content of chemical composition was calculated by the peak area normalization method. [Results]29 kinds of chemical compounds were identified in volatile oils from underground part of Origanum vulgare L,accounting for 98. 97% of total volatile oils; among these 29 kinds of chemical compounds,10 were the first time report. [Conclusions]Apart from containing antibiotic composition as aerial part,the underground part of Origanum vulgare L also contain many fatty acid chemical compounds;such substances have strong antioxidant activities and should not be neglected,and have certain medicinal value.

    2016年Z1期 v.7 1-3页 [查看摘要][在线阅读][下载 218K]
  • Thin Layer Chromatography and Methodological Investigation of Geniposide in Zhukuqin Granule

    Wenjie ZHONG;Zhaorong ZHU;Juan LIU;Yichen LUO;

    [Objective] To screen the thin layer chromatography conditions for geniposide in Zhukuqin granule,and to establish quality control method for geniposide. [Methods] Sample was treated by different solvents and operation methods. With ethyl acetate-acetone-formic acid-water( 5∶ 5∶ 1∶ 1) as the developing agent and 10% ethanol sulfate as the color developing agent,system applicability investigation was carried out. [Results]After ultrasound and extraction treatments,Zhukuqin solution in thin layer chromatography had the same principal spots as the reference substances. Rf value of geniposide was 0. 678 4 ± 0. 011 3; and resolution degree was 1. 395 ± 0. 04119. Spot color contrasted well with the background color. [Conclusions]The established method had good repeatability and strong specificity,and could be used for the quality control of Zhukuqin granule.

    2016年Z1期 v.7 4-7+10页 [查看摘要][在线阅读][下载 833K]
  • Fingerprints Analysis of the Chemical Components of Chloroform Parts of Renqing Mangjue Compatibility with Zuota or Not

    Zhongjie HUANG;Bo LI;Shaoshan ZHANG;Mengjie YU;Yuan LIU;

    [Objectives] To research the fingerprints of chemical components of chloroform parts of Renqing Mangjue and Renqing Mangjue powder( compatibility without Zuota),and to identify Renqing Mangjue compatibility with Zuota or not. [Methods]High-performance liquid chromatography was adopted. Chromatographic column was DIKMA diamonsil( 4. 6 mm × 250 mm,5 μm); flow rate was 1 mL / min; column temperature was 30 ℃; mobile phase was methanol-water with gradient elution. [Results] A total of 18 common fingerprint peaks were obtained. As for the petroleum ether parts of Renqing Mangjue and Renqing Mangjue powder( compatibility without Zuota),eight characteristic peaks were obtained. [Conclusions] Characteristic fingerprints of chloroform parts of Renqing Mangjue and Renqing Mangjue powder( compatibility without Zuota) could be obtained by the high-performance liquid chromatography. Therefore,the Renqing Mangjue and Renqing Mangjue powder( compatibility without Zuota) could be identified according to their characteristic peaks.

    2016年Z1期 v.7 8-10页 [查看摘要][在线阅读][下载 290K]
  • Principal Component Analysis of Volatile Oils of FLOS DENDRANTHEMA INDICI

    Lijuan WAN;Jinqing LU;Xiaoshuang LI;Jie LIN;Han TU;Junjie XU;

    [Objective] To research the principal component of volatile oils in FLOS DENDRANTHEMA INDICI,and to provide scientific references for the material basis of pharmacological action of FLOS DENDRANTHEMA INDICI. [Methods] A total of 15 batches of FLOS DENDRANTHEMA INDICI were collected. Volatile oils were extracted by steam distillation method,and analyzed by GC-MS. Comprehensive evaluation was carried out by principal component analysis. [Results]The original information of 23 indexes was summarized by five principal components,including 88. 966% information of the original index. The first principle component( PC1) could be used as the descriptive index of antiasthmatic effects of volatile oils in FLOS DENDRANTHEMA INDICI; the second and third principle components( PC2 and PC3) could be used as the descriptive indexes of analgesic effects and cough relieving effects,respectively. [Conclusions] The common components of volatile oils in FLOS DENDRANTHEMA INDICI were analyzed by principal component analysis,which provided theoretical reference and practical exploration for the antiasthmatic effects,analgesic effects and cough relieving effects of volatile oils in FLOS DENDRANTHEMA INDICI.

    2016年Z1期 v.7 11-14+18页 [查看摘要][在线阅读][下载 440K]
  • GC-MS Fingerprint of the Volatile Oils of FLOS DENDRANTHEMA INDICI

    Jie LIN;Jinqing LU;Hanmei JIANG;Xiaoshuang LI;Han TU;Kun ZHOU;

    [Objective] To establish GC-MS fingerprint determination method for volatile oils of FLOS DENDRANTHEMA INDICI,and to provide references for the quality control of FLOS DENDRANTHEMA INDICI. [Methods] Volatile oils were extracted from FLOS DENDRANTHEMA INDICI by steam distillation. Fingerprint of FLOS DENDRANTHEMA INDICI was detected by GC-MS technology. Chinese herbal fingerprint calculation software was used to calculate the data. Common fingerprint of volatile oils in FLOS DENDRANTHEMA INDICI was established. [Results] Volatile oils in FLOS DENDRANTHEMA INDICI contained 23 characteristic index components; GC-MS fingerprint was established with these 23 common peaks as the characteristic fingerprint information. [Conclusions]This method was precise,stable and repeatable; the established volatile oils fingerprint laid foundation for the quality control of FLOS DENDRANTHEMA INDICI.

    2016年Z1期 v.7 15-18页 [查看摘要][在线阅读][下载 366K]
  • Determination of 8 Kinds of Organochlorine Pesticide Residues in the Leaves of Clausena lansium by Gas Chromatography

    Yong WANG;Shuo CHEN;Weiyong LAI;Junqing ZHANG;Zeyou LI;

    [Objective] To establish a method for the determination of 8 kinds of organochlorine pesticide residues in the leaves of Clausena lansium by gas chromatography( GC). [Methods]The samples were macerated in water for overnight,extracted with acetone,dichloromethane and hexane by ultrasonic wave. The extract solvent was purified by concentrated sulphuric acid. The separation was performed on a DB-1701 capillary column through a temperature programme and detected by an electron capture detector. 8 kinds of organochlorine pesticide residues including the four isomers of BHC and the four isomers of DDT were determined in the samples. [Results] The average recoveries were in the range of 91. 23% and 98. 56%,and their RSD values were 1. 34- 3. 66%. [Conclusion]The established method is simple,quick,low in cost and easy-operating,suitable for the determination of organochlorine pesticide residues in the leaves of C. lansium.

    2016年Z1期 v.7 19-21+26页 [查看摘要][在线阅读][下载 159K]

Processing of Traditional Chinese Medicine and Quality Control

  • Effects of Preculture on Cryopreservation of Nodes from Dioscorea opposita Thunb. cv. Tiegun Plantlets by Encapsulation-vitrification

    Xiting ZHAO;Ping LI;Liwei JIANG;Yuting ZHU;Zhihui SONG;Linlin HAN;Mingjun LI;

    [Objective]To research the effects of preculture on the cryopreservation by encapsulation-vitrification of Dioscorea opposita Thunb.cv. Tiegun. [Methods] Tissue culture method was adopted; all the preculture tests were carried out under 4 ℃. [Results]Sucrose concentration,penetrant types,preculture time and preculture method had significant impacts on the relative survival rate and regeneration rate of D.opposita nodes after cryopreservation by encapsulation-vitrification. The appropriate sucrose concentration and precultured time were 0. 2 mol /L and 3 d,respectively. When 0. 1 mol / L sucrose + 0. 1 mol / L glycerol was taken as the penetrant,the relative survival rate and regeneration rate were the maximum after cryopreservation by encapsulation-vitrification. Effect of direct preculture for 3 d on MS + 0. 1 mol / L sucrose +0. 1 mol / L glycerol was the best,which could obtain the highest relative survival rate( 89%) and regeneration rate( 52%) after cryopreservation. Therefore,plantlet nodes of D. opp. Thunb. cv. Tiegun should be precurtured on MS + 0. 1 mol / L sucrose + 0. 1 mol / L glycerol for3 d at 4 ℃. [Conclusions] This research provides a technology system for vitrification,and the method is simple and effective.

    2016年Z1期 v.7 22-26页 [查看摘要][在线阅读][下载 477K]
  • Optimization of Extraction Technology for Total Saponins from Allium wallichii Kunth by Orthogonal Test

    Panpan LIU;Yunzhang XU;Fenglin WANG;Yun TENG;Xue ZHAI;Ying LI;Yuan LIU;

    [Objective] To optimize the extraction technology of total saponins from Allium wallichii Kunth. [Methods]Effects of liquid-solid ratio,ethanol concentration,extraction temperature and extraction time were researched by single factor test. Extraction technology of total saponins from A. wallichii Kunth was optimized by L9( 34) orthogonal test. [Results]The optimal extraction technology was 50∶ 1 mL /g liquid-solid ratio,60% ethanol concentration,2. 5 h extraction time and 50 ℃ extraction temperature. Under this condition,the extraction rate of total saponins from A. wallichii Kunth was 1. 20%. [Conclusions] This extraction technology was simple and convenient,and could be used for the extraction of total saponins from A. wallichii Kunth.

    2016年Z1期 v.7 27-29+34页 [查看摘要][在线阅读][下载 383K]
  • Optimization of Extraction Technology of Total Flavonoids from HERBA CENTIPEDAE by Orthogonal Test

    Dengfeng ZOU;Bingxiong PAN;Suyi CHEN;Aize XIE;

    [Objective] To optimize the extraction technology of total flavonoids from HERBA CENTIPEDAE by orthogonal test. [Methods]Content of total flavonoids was detected by ultraviolet spectrophotometry. With the extraction rate of total flavonoids as the index,effects of enzymolysis time,pH value,cellulase concentration and enzymolysis temperature were researched. [Results] The optimal extraction technology was as follows: pH = 5,2. 5 h enzymolysis time,0. 3 mg / mL cellulase concentration and 37 ℃ enzymolysis temperature. Under this condition,the extraction rate of total flavonoids was 2. 814 7%. [Conclusions] This method was stable,easy and controllable,and provided references for the further development and utilization of HERBA CENTIPEDAE.

    2016年Z1期 v.7 30-31+39页 [查看摘要][在线阅读][下载 353K]

Pharmacological and Toxicological Analyses

  • Anti-inflammatory Mechanism of HERBA COMMELINAE

    Xin YU;Lilan OU;Siwei CHEN;Dan ZHANG;

    [Objective] To research the anti-inflammatory mechanism of HERBA COMMELINAE. [Methods] Contents of MDA,PGE2,TNF-α and IL-1β were detected in chest exudate of rats,as well as the contents of NO,PGE2,TNF-α,IL-1β and MDA in lung tissues. The anti-inflammatory mechanism was discussed. [Results] Compared with 5% Tween-80 group,HERBA COMMELINAE ethanol crude extracts group and water soluble part group reduced the MDA,PGE2,TNF-α and IL-1β contents in inflammatory exudate of rats chest( P < 0. 01),and restricted the increase of NO,MDA,PGE2,TNF-α and IL-1β contents of lung tissues( P < 0. 01). [Conclusions] The anti-inflammatory mechanism of HERBA COMMELINAE might be related to the restriction of antioxidation and inflammatory mediator generation.

    2016年Z1期 v.7 32-34页 [查看摘要][在线阅读][下载 125K]
  • Structural Modifacation and Antioxidant Activity of Pachymaran

    Xiaohong CHEN;Fang MA;Yunbin YAN;Biaosheng LIN;Shaoxin SHEN;

    Structure modification of carboxymethylation and ferulic acid in pachymaran of PORIA were carried out. Antioxidation activities in vitro and vivo of carboxymethyl pachymaran and pachymaranferulate were detected. Within the effective cocnentration range,carboxymethyl pachymaran and pachymaranferulate still had certain reducing capacity and antioxidant capacity. Their reducing capacity and scavenging activity showed significant dose-effect relationship with sample concentration. And when the concentration reached a certain level,both reducing capacity and scavenging activity became stable. Antioxidant capacity of pachymaranferulate was slightly stronger than that of carboxymethyl pachymaran,but was weaker than ascorbic acid. Besides,scavenging actions of carboxymethyl pachymaran and pachymaranferulate to ·O-2were weaker than ·OH.

    2016年Z1期 v.7 35-39页 [查看摘要][在线阅读][下载 672K]

Germplasm Resources and Cultivation

  • Germplasm Resource Collection and Fruit Yield Comparison of Schisandra chinensis (Turcz.) Baill.

    Shunjie ZHANG;Aihua CHEN;Yu CHEN;Chan QIAO;

    [Objective] This study aimed to collect germplasm resources of Schisandra chinensis( Turcz.) Baill. from three northeastern provinces of China and compare fruit yield of S. chinensis( Turcz.) Baill. individuals from different provenances. [Methods] Germplasm resources of S. chinensis( Turcz.) Baill. from 13 different provenances were collected as experimental materials to investigate morphological parameters and measure fruit yield.[Results] According to the statistical results,S. chinensis( Turcz.) Baill. individuals from Raohe provenance exhibited the highest degree of lignification,while those from Dalishu provenance exhibited the lowest degree of lignification. Within the same year,fruit yield of S. chinensis( Turcz.) Baill. individuals from different provenances varied significantly; dry fruit weight and fresh fruit weight exhibited the same variations trend. In different years,S. chinensis( Turcz.) Baill. individuals from different provenances exhibited inconsistent fruit yield. Fresh fruit weight and dry fruit weight of S. chinensis( Turcz.) Baill. individuals from different provenances exhibited extremely significant differences( P < 0. 01); ratio of fresh fruit weight to dry fruit weight of S. chinensis( Turcz.) Baill. individuals from different provenances exhibited significant differences( P < 0. 05). The ratio of fresh fruit weight to dry fruit weight of S. chinensis( Turcz.)Baill. individuals from different provenances exhibited significant differences.[Conclusions]This study provides theoretical basis for breeding high-quality varieties of S. chinensis( Turcz.) Baill.

    2016年Z1期 v.7 40-42+45页 [查看摘要][在线阅读][下载 149K]
  • Genetic Diversity of RADIX PUERARIAE in Chongqing Area and the Cluster Analysis of Puerarin

    Junyi CHEN;Baoyong ZHANG;Li TAN;Yahong ZHANG;Xiaoke HUANG;Xu JING;Ding LAN;

    RAPD analysis of the genetic diversity of 12 germplasm resources of RADIX PUERARIAE in Chongqing Area was carried out,as well as its puerarin cluster analysis. Research results showed that according to the RAPD analysis,genetic distance of RADIX PUERARIAE was between 0. 00 and 0. 36,with mean value being 0. 19. The genetic similarity coefficients of RADIX PUERARIAE germplasm resources were classified into three groups by group-average method. And puerarin contents were divided into three groups by cluster analysis.

    2016年Z1期 v.7 43-45页 [查看摘要][在线阅读][下载 272K]

Biotechnology

  • Diversity Analysis of Endophytic Fungi in Mentha Using Illumina MiSeq High-throughput Sequencing Technology

    Zebin CHEN;Tiyuan XIA;Dingkang WANG;Guoxing WU;Jiani LIU;Yongfu ZHANG;Zhen REN;Song JIN;Shengjing PENG;Yanfen NIU;

    [Objective] This study aimed to investigate the diversity of endophytic fungi in Mentha. [Methods] By using Illumina MiS eq high-throughput sequencing technology,the 18 S rD NA-V4 region of endophytic fungi in Mentha was sequenced. Qiime and Mothur software packages were used to sort and count the number of sequences and operational taxonomic units( OTUs) and analyze the species abundance and distribution,Alpha diversity and differences of species abundance among samples. [Results]The number of effective sequences and OTUs for each sample was 64 179 and 15,respectively. The rarefaction curve showed that the sequencing deepness was large enough. The number of OTUs had almost reached a saturation point. In Mentha( BH) samples,Chao1 index and Shannon-Wiener index were 15. 0 and 0. 242,respectively. Endophytic fungi in Mentha mainly belonged to the genera Davidiella( 11. 72%) and Pleurotus( 61. 86%),but the classification status of 26. 42% of fungi had not been identified. Pleurotus was the dominant genus of endophytic fungi in Mentha. [Conclusions]Illumina MiS eq high-throughput sequencing technology provides more accurate and scientific data resources for the study of endophytic fungi in Mentha.

    2016年Z1期 v.7 46-50页 [查看摘要][在线阅读][下载 409K]

  • Medicinal Plant

    <正>Indexed byChemical Abstracts(CA),AGRIS Database,CAB International(CABI),China National Knowledge Infrastructure(CNKI),Naresuan University Library,Indian Fund for Agricultural Development,Royal Tropical Institute,Library of Congress,ProQuest So far,a total of more than 3000 users have subscribed to Medicinal Plant,distributed in 6 countries and regions.

    2016年Z1期 v.7 2页 [查看摘要][在线阅读][下载 779K]
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