Pharmacological and Toxicological Analyses

  • Hypoglycemic Effects of the n-Butanol Part of Sorbus tianschanica

    Le LI;Xianghui SU;Hui TANG;

    [Objective] To extract and isolate the effective components from Sorbus tianschanica,and to research the hypoglycemic effects of n-Butanol part. [Methods]S. tianchanica was extracted by reflux extraction. After gradient elution,n-Butanol part was obtained. With diabetic mice induced by STZ as the model,blood glucose test was carried out. [Results]n-Butanol part in administration group had significant differences in hypoglycemic effects with that in model group(P < 0. 05),showing that n-Butanol part had certain antidiabetic functions on diabetic mice induced by STZ. And the hypoglycemic effects showed a reducing trend as the administration dosage decreased,indicating that administration dosage had positive correlation with hypoglycemic effects. n-Butanol in different parts could reduce the blood glucose of diabetic mice in different degrees. And the blood glucose value in high-dosage group(8 g /kg) reduced greatly at 2 h after administration,having great differences with that in model group(P < 0. 01). This showed that high dosage was helpful to the glucose tolerance of diseased mice,and could effectively protect against the rapid increase of blood glucose after meal. n-Butanol had no significant effects on the blood glucose of normal mice. However,the morality rates in high-and middle-dosage groups were both higher than that in low-dosage group; and the morality rate in administration group was lower than that in model group,showing that n-Butanol could promote the survival rate of diabetic mice. [Conclusions]S. tianchanica had certain effects of anti-diabetes.

    2014年05期 v.5 1-2+8页 [查看摘要][在线阅读][下载 91K]
  • Evaluating the Influence of Veratrum Nigrum to Panax Ginseng Based on Uniform Design

    Ya LIN;Ying XU;Erxin SHANG;Yue YANG;Fengrui SONG;Zhiqiang LIU;Na LIN;

    [Objective] To inspect the influence of Veratrum Nigrum on the body resistance strengthening function of Panax Ginseng with different proportion and dosages. [Methods] ICR mice were divided,according to their weights,into normal group,model group and Panax Ginseng group of different dosagess. Immunocompromised mouse were constructed by intraperitoneal injection with cyclophosphamide(45 mg/kg,qod × 3). Medicines were administrated since the first day of modeling,once per day for 7 days in total,so as to find out the effective dose range of Panax Ginseng. With uniform design and medicine administration with 2 factors 11 levels,observing the influences of different proportions and dosages of combined decoctions of Panax Ginseng and Veratrum Nigrum through the index of immune organs(spleen and thymus),ability of carbon clearance and blood routine of the modeling mice. [Results]Comparing with the mice in nnormal group,ones in modeling group had obvious reduction in the body mass,visceral index,phagocytic function and blood routine. Splenic index,phagocytic correction index,WBC,RBC and Hb increased significantly with only using Panax Ginseng(0. 5- 8. 0 g /kg). Evaluation of the influence of Veratrum Nigrum on the body resistance strengthening function of Panax Ginseng based on uniform design:(1) Influence on the index of immune organs:Decoctions(Veratrum Nigrum + Panax Ginseng 0. 53- 8. 05 g /kg) had no obvious effect on the index of immune organs of immunocompromised mice.(2) Influence on the ability of carbon clearance: Veratrum Nigrum could reduce the ability of Ginseng to enhance the phagocytic function(Panax Ginseng∶ Veratrum Nigrum < 100 ∶1) in direct proportion. The strongest antagonistic effect of Veratrum Nigrum would be achieved when the total dose of decoctions was 3. 08 g/kg(Panax Ginseng∶Veratrum Nigrum =9∶1).(3) Influence on blood routine: if Panax Ginseng∶Veratrum Nigrum <100∶1,the effect of Ginseng to increase WBC and RBC would be counteracted by Veratrum Nigrum(0. 034-0. 13 g/kg)without influencing the dose-effect curve of Ginseng. The strongest antagonistic effect of Veratrum Nigrum would be achieved when the total dose of decoctions was 4. 58 and 3. 48 g /kg(Panax Ginseng∶ Veratrum Nigrum = 9∶1). The effect of Ginseng to increase Hb would also be counteracted by Veratrum Nigrum but the dose-effect curve of Panax Ginseng would decline. [Conclusions]Within the dose range stipulated in the Pharmacopoeia,the body resistance strengthening function of Panax Ginseng may be reduced or counteracted by Veratrum Nigrum,and the intensity of effect changed with the imparity of proportions and dosages.

    2014年05期 v.5 3-8页 [查看摘要][在线阅读][下载 254K]
  • The Protective Effect of EtOAc Extract from Gastrodia elata on Oxidative Damage of PC_(12) Cells

    Chunni HAN;Hanwen YAN;Jinlong WEN;Qing LIN;Xiaohua DUAN;

    [Objective] The purpose of this study was to investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of PC12cells induced by H2O2. [Methods]PC12cells were cultured and exposed to H2O2to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification,colorimetric MTT assay,leakage rate of LDH,intracellular ROS level and T-SOD activity. [Results]Compared with the model group,EtOAc extract from Gastrodia elata could improve the cells morphology significantly,increase the survival rate of 14. 7%(P < 0. 01),decrease the ROS level(P < 0. 01) and the release of LDH(P <0.01) from cells into culture medium,raise intracellular T-SOD activity(P <0.01). [Conclusions]EtOAc extract from Gastrodia elata had obviously antioxidant effect in vitro.

    2014年05期 v.5 9-12+17页 [查看摘要][在线阅读][下载 198K]
  • Study on the Effect of APS on the Proliferation and Cytokines Secretion of the Bone Mesenchymal Stem Cells Drived from Patient with Multiple Myeloma

    Hai BAI;Lin LIU;Cunbang WANG;Yaozhu PAN;Rui XI;Yuping WEI;

    [Objectives] In this study,the influence of astragalus polysaccharide on proliferation and cytokine expression of bone mesenchymal stem cells( abbreviated as BMSC) of patients who were attacked by multiple myeloma( abbreviated as MM) would be analysed. [Methods]MM bone marrow mesenchymal stem cells(MM-BMSC) and normal human bone marrow mesenchymal stem cells(ND-BMSC) were isolated by the method of density gradient centrifugation. The 3rdgeneration BMSC was taken and measured growth curve and cytokine expression of cells. Respectively by MTT,flow cytometry,the influence of different concentrations of APS on cell proliferation and cell cycle of MM-BMSC. The method of ELISA was ultilized to meaure the expression levels of IL-1β and IL-6. [Results]MM-BMSC and ND-BMSC were successfully isolated and obtained. The cell growth curve showed MM-BMSC proliferated slowly. Its doubling time was of 86 h while the ND-BMSC doubling time was of 60 h,which comparatively,exsited statistically significant difference(P < 0. 05). Normally on the basis,levels of secreted IL-1β and IL-6 between MM-BMSC and ND-MSC exsited significant difference(P < 0. 05). The proliferation of MM-BMSC could be promoted by APS,in which the role of 1 mg /mL APS could reach the optimum(P < 0. 05). MM-BMSC basically kept in the state of stationary phase. If 1 mg /mL MM-BMSC disposed by APS was in the period of G0/G1,comparatively,the cell proportion was significantly lower(P <0. 05). While in the period of G2,the cell proportion was significantly higher(P <0.05). Furthermore,1 mg/mL APS could significantly reduce the levels of IL-1β and IL-6(P < 0. 05) secreted by MM-BMSC. [Conclusions]The levels of IL-1β and IL-6 secreted by bone marrow mesenchymal stem cells of multiple myeloma patients were in the state of abnormality. Meanwhile,1 mg /mL astragalus polysaccharides could promote the proliferation of bone marrow mesenchymal stem cells and lower its secretion of IL-1β,IL-6.

    2014年05期 v.5 18-21+26页 [查看摘要][在线阅读][下载 226K]
  • Effects of Resveratrol on Proliferation and Cell Cycle of HepG-2 Cell

    Shengjiu GU;Dan CHEN;Junbin QI;Meibo LI;Yourui XU;Kaimei ZHU;

    [Objective] To discuss the effects of resveratrol on the proliferation and cell cycle of human hepatoblastoma-derived HepG-2 cell line. [Methods]The resveratrol was extracted by microwave-assisted method and HepG-2 cells were exposed to resveratrol of different concentrations which were 12. 5,25,50 and 100 μmol /L. The inhibiting effect of resveratrol on the proliferation of HepG-2 cells was measured by MTT. Morphologic changes of cells were detected by the phase contrast microscope. The cell cycle of HepG-2 cells treated with resveratrol were analyzed by flow cytometry. [Results]Resveratrol could significantly inhibit proliferation of HepG-2 cells and the effect was time and concentration dependent. According to results of FCM,resveratrol could block HepG-2 cells into S phase and the effect was dose dependent. [Conclusions]Resveratrol could inhibit the proliferation of HepG-2 cells and induce apoptosis. It could induce cell blockage in S phase.

    2014年05期 v.5 27-30+33页 [查看摘要][在线阅读][下载 203K]
  • Anti-oxidative Activity of Flavonoids from Cherry Leaves in Vitro

    Shan ZHENG;Feng ZHAO;Zucheng LI;Yuanfang WANG;

    [Objective] This study was undertaken to extract flavonoids from cherry leaves and to investigate the anti-oxidative activity in vitro. [Methods]The scavenging action on OH·and O-2·and its inhibiting effect on oxidative hemolysis of erythrocyte induced by H2O2and lipid peroxidation of hepatic tissue in mice were investigated in vitro. [Results] The flavonoids showed concentration-dependent scavenging effect on OH·and O-2·. The flavonoids also showed remarkable inhibiting effects on oxidative hemolysis of erythrocyte in mice induced by H2O2. When the final concentration of the flavonoids reached 30%,the inhibition rate was 74. 13%. It could inhibit lipid peroxidation of hepatic tissue in mice induced by vitamin C with low concentration and ferrous sulfate. And the inhibiting effect was concentration-dependent.When the final concentration of the flavonoids reached 30%,the inhibition rate was 68. 33%. [Conclusions]The total flavonoids from cherry leaf have concentration-dependent antioxidant activity in vitro.

    2014年05期 v.5 43-45+48页 [查看摘要][在线阅读][下载 122K]
  • The Anti-inflammatory Effects of CORTEX PHELLODENDRI in Acute Inflammation Animal Model

    Lilan OU;Xin YU;Ye ZHU;Chun ZHANG;

    [Objective] To research on the anti-inflammatory effects of CORTEX PHELLODENDRI in acute inflammation animal model.[Methods]Two acute inflammation animal models were established,including the auricle swelling test model of mice induced by xylene,and the abdominal capillary permeability model of mice induced by 1% glacial acetic acid. The anti-inflammatory effects of CORTEX PHELLODENDRI were observed. [Results]CORTEX PHELLODENDRI had different inhibitory effects on the auricle swelling of mice induced by xylene,and the abdominal capillary permeability of mice induced by 1% glacial acetic acid. The alkaloid part had significant differences with model group(P < 0. 05 or P < 0. 01). [Conclusions]Alkaloid part had good anti-inflammatory effects; while the effects of n-butanol,ethyl acetate,chloroform and petroleum ether parts were in different degrees.

    2014年05期 v.5 46-48页 [查看摘要][在线阅读][下载 90K]

Processing of Traditional Chinese Medicine and Quality Control

  • Research on the Technology of Ultrasonic Assisted Extraction of Flavonoids from the Stems and Leaves of Pratia begoniifolia

    Lunpei LIU;Libo LIU;Xianhui SHAO;Fang LIAO;

    [Objective] To study the optimal technology of ultrasonic assisted extraction of flavonoids from the stems and leaves of Pratia begoniifolia. [Methods]Ethanol with the concentration of 70% was taken as the reagent,and ultrasonic was used as an auxiliary extraction equipment. Main factors influencing extraction of flavnoids and the optimal level range of the factors were determined by single factor experiment,and the optimal extraction conditions were determined by L9(34) orthogonal test. [Results]Factors influencing extraction of flavnoids from the stems and leaves of Pratia begoniifolia Lindl. from primary to secondary were: ultrasonic power > leaching temperature > extraction duration >ratio of solid to liquid. The optimal conditions were: the ultrasonic power was 350 W,the leaching temperature was 60 ℃,the extraction duration was 30 min,and the ratio of solid to liquid was 1∶20(g /mL). Under these conditions,the content of flavonoids in stems and leaves of Pratia begoniifoli was 38. 437 mg /g. [Conclusions]The optimum conditions gained from the experiment can provide basis for the development and utilization of Pratia begoniifolia.

    2014年05期 v.5 13-17页 [查看摘要][在线阅读][下载 132K]
  • Screening and Optimization of the Extracting Technology for GANODERMA Capsule

    Jie ZHANG;Dingwen JIANG;Ying HE;Wei CHEN;Qingrong WANG;Xianrong SHEN;Jianxiang LI;

    [Objective] To screen and optimize the extracting technology for GANODERMA capsule. [Methods]Lymphocyte transformation test in mice induced by Con A was used to screen the three extracting technologies,which were ethanol-extracting technology,water-ethanol extracting technology and water-extracting technology. With extraction rate and total amount of GANODERMA polysaccharides as the investigation indices,orthogonal test was designed. Effects of water volume,extraction temperature and extraction time on the extraction technology of GANODERMA were investigated. [Results]Results of animal test showed that water-extracting technology had the optimal effects of immunoregulation on mice. And the optimal water-extracting technology of GANODERMA capsule was as follows: after two times of boiling for GANODERMA at 100 ℃,the first extraction was carried out according to 1∶ 20 solid- liquid ratio(g /mL); then reflux extraction was carried out for 2 h after boiling; after filtration,1∶ 15 solid- liquid ratio(g /mL) was carried out for the second extraction,then reflux extraction was adopted for2 h after boiling; the second extraction was carried out according to 1∶ 15 solid-liquid ratio(g /mL),then reflux extraction was adopted for1 h after boiling. [Conclusions]Water-extracting technology for GANODERMA capsule had the optimal effects of immunoregulation; and the selected extraction technology was reasonable and feasible.

    2014年05期 v.5 49-52页 [查看摘要][在线阅读][下载 92K]

Chemical Composition of Natural Medicine

  • HPLC-DAD-ELSD Simultaneous Determination of Four Triterpenoids in RHIZOMA ALISMATIS

    Jianfang QIU;Jing LIN;Wen XU;Mingqing HUANG;Wanli ZHAO;Fenrong LUO;Xiaoyan LI;Shuisheng WU;

    [Objective] To establish an analytical method for simultaneously determining the alisol C 23-acetate,Alisol A,alisol B,alisol B23-acetate in RHIZOMA ALISMATIS. [Methods]The assay was performed on a Ultimate XB- C18column(4. 6 mm × 250 mm,5 μm) with the mobile phase consisting of acetonitrile- water(65∶35) at a flow rate of 1. 0 mL /min. The column temperature was at 30 ℃ and the optimum detection wavelength of DAD was 245 nm. The drift tube and atomizer temperatures of ELSD were 60 and 55 ℃,respectively,with a carrier gas(N2) pressure of 20 psi. [Results]The linear range of alisol C 23-acetate,Alisol A,alisol B and alisol B 23-acetate were 1.869-29. 90 μg /mL(r = 0. 999 8),3. 731- 74. 62 μg /mL(r = 0. 999 7),8. 653- 138. 4 μg /mL(r = 0. 999 9),and 4. 832- 77. 31 μg /mL(r =0. 999 5),respectively. The average recovery rates(n = 6) were 98. 78%(RSD = 2. 63%),98. 05%(RSD = 2. 72%),98. 26%(RSD =2. 86%) and 97. 65%( RSD = 2. 95%),respectively. 20 batches of RHIZOMA ALISMATIS were detected by this method; and results showed that there were relatively great differences in the contents of four triterpenoids between RHIZOMA ALISMATIS grown in Sichuan and Fujian. The average content of total triterpenoids in 10 batches of RHIZOMA ALISMATIS grown in Sichuan was significantly higher than that grown in Fujian. The content of former was 2. 499- 4. 701 mg /g; while the content of latter was 1. 210- 3. 523 mg /g. Besides,alisol A was not detected in the RHIZOMA ALISMATIS grown in Fujian Province; while the alisol A content in RHIZOMA ALISMATIS grown in Sichuan was relatively high. Therefore,this feature could be used for the identification of two RHIZOMA ALISMATIS. [Conclusions] The HPLCDAD-ELSD quantitative analysis method was simple,rapid and accurate,and could be used for the quantitative analysis of multi-component of RHIZOMA ALISMATIS,which provided a novel approach for the comprehensively evaluation of the quality of RHIZOMA ALISMATIS.

    2014年05期 v.5 22-26页 [查看摘要][在线阅读][下载 133K]
  • Study on the Plant Morphology and Total Polysaccharides Content in the Stems of Panax quinquefolius L.Grown in Canada

    Zhengming YANG;Yanfei HUANG;Bo LI;Panpan LIU;Yijun CHEN;Yuan LIU;

    [Objective] To research on the changes of total polysaccharides content and plant morphology in the 1- to 5-year-old stems of Panax quinquefolius L.,which were harvested from May to September in Canada. [Methods]Plant morphological characteristics(length,diameter,dry weight and fresh weight) of the stems of P. quinquefolius with different growth years in different collection months were detected;and the dry rate was calculated. Total polysaccharides content in the stems of P. quinquefolius was detected by UV spectrophotometry; and DPS2000 was used for data analysis. [Results]Plant morphological characteristics of the stems of P. quinquefolius showed a increasing trend as the growth years and harvest period prolonged. dry rates of P. quinquefolius stems with different growth years had significant differences(P=0.013 1 <0.05),which were in the order of one-year-old stems(0.348) > two-year-old stems(0.296) > five-year-old stems(0.202) >four-year-old stems(0. 174) > three-year-old stems(0. 164). dry rates of P. quinquefolius stems in different harvest periods showed extremely significant differences(P = 0. 005 8 < 0. 01),which were in the order of July > May > June > August > September. Total polysaccharides contents of P. quinquefolius stems had extremely significant changes in different growth years(P = 0. 000 0 < 0. 01),which were in the order of two-year-old stems(30. 818 mg /g) > one-year-old stems(28. 172 mg /g) > three-year-old stems(26. 642 mg /g) > four-year-old stems(24.89 mg/g) > five-year-old stems(19.492 mg/g). Total polysaccharides contents of P. quinquefolius stems also had extremely significant changes in different harvest periods(P = 0. 000 0 < 0. 01),which were in the order of June > July > August > May > September. Content of total polysaccharides had little correlation with the dry rate. Therefore,the content of total polysaccharides could not be judged by the dry rate of the stems of P. quinquefolius. [Conclusions]The stem yield and polysaccharides content of P. quinquefolius were both at a relatively high level. Therefore,it was suggested that the stems of P. quinquefolius could be used as the extraction raw materials of total polysaccharides,considering from the angle of comprehensive utilization of P. quinquefolius.

    2014年05期 v.5 34-37页 [查看摘要][在线阅读][下载 135K]
  • Effect of Dry Methods on the Content of Ligustilide in Miwu.

    Xin YU;Lilan OU;Ye ZHU;Na SANG;Dan ZHANG;

    [Objective] To study the effect of dry methods on the content of ligustilide in Miwu. [Methods]The content of ligustilide in Miwu. dried by different methods was determined by HPLC. [Results]There was a good linear relationship( r = 0. 999 9) between ligustilide and peak area when the sample injection was 0. 019 97- 0. 199 7 μg /mL,the average recovery rate was 100. 2% and RSD was 1. 09%(n =9). Different dry methods had a certain degree of effect on the ligustilide content in Miwu. [Conclusions]Oven-drying after drying in the sun is the best among the three different dry methods.

    2014年05期 v.5 38-40页 [查看摘要][在线阅读][下载 103K]
  • Content Detection of Total Flavonoids in Melastom adodecandnun Lour.

    Xiaofu LIU;Ming LI;Kefeng ZHANG;

    [Objective] To establish a content detection method for the total flavonoids in Melastom adodecandnun Lour. [Methods]Content of total flavonoids in M. adodecandnun was detected by UV-Vis spectrophotometry. [Results]When the content of total flavonoids in M. adodecandnun was detected by UV-Vis spectrophotometry,there was good linear relationship(r = 0. 999 6) within the range of 0. 004 16- 0. 066 59mg/mL. The average recovery rate was 98. 85% and RSD was 2. 75%. [Conclusions]This method was accurate,simple and could be used for the content detection of total flavonoids in M. adodecandnun.

    2014年05期 v.5 41-42页 [查看摘要][在线阅读][下载 77K]
  • Enzyme Extraction and Pre-column Derivation HPLC Analysis of Rose Polysaccharides

    Yuehui ZHANG;Liwen HAN;Lan ZHENG;Jianguo SHI;Junhui YANG;Yaohong MA;Yingying DUAN;Wenhong FENG;Shengmin ZHOU;Dapeng CAO;Wei ZHAO;

    [Objective] To screen the extraction method of rose polysaccharides,and to provide theoretical foundation for the reuse of residues after extracting the essential oils from rose. [Methods]Rose polysaccharides were extracted by enzyme method. Pre-column derivation HPLC was used to analyze the contents. [Results]The polysaccharides extracted from dry rose and rose residues by normal method were 98. 65 and50. 87 mg /g,respectively. After pretreated by enzyme,the polysaccharides were 124. 87 and 75. 99 mg /g. The monosaccharides in rose polysaccharides were analyzed by pre-column derivation HPLC. And the rose polysaccharides were mainly composed by mannose,rhamnose,galacturonic acid,glucose,galactose and arabinose. And their molar ratio was 1∶ 2. 2∶ 6. 0∶ 2∶ 7. 9∶ 8. 0. The sugar composition of primary structure of rose polysaccharides extracted by enzyme method was the same as that extracted by normal method(not adding enzyme). [Conclusions]Useful rose polysaccharides could be extracted from the residues by enzyme method.

    2014年05期 v.5 53-55+58页 [查看摘要][在线阅读][下载 129K]


  • Effect of Colohicine Treatment on the Growth and Variation of Siraitia grosvnorii Callus

    Fan LIU;Zhigang YAN;Longhua BAI;Xiaojun MA;Ying WEI;

    [Objective] This study was to screen out a suitable colchicine treatment method for generating variation in calli of Siraitia grosvnorii. [Methods]Different concentrations of colchicine and treatment time were set to investigate the optimal callus induction conditions for Siraitia grosvnorii(Swingle) C. Jeffrey. [Results]With the increase of colchicine concentration and time,the calli died more and more seriously. The induction rate of mutated cells( polyploidy) was ascended along with the increase of treatment time and colchicine within a certain range. However,when the colchicine concentration exceeded a specific amount,the polyploidy induction rate was decreased. Treatment with0. 5 g /L colchicine for 48 h showed the best effect on polyploidy induction while the least damage on callus tissue. In comparison with the normal material,the mutated plants showed bigger leaves and slower growth,while the stomata did not change remarkably. By chromosomal analysis,the mutated material was preliminarily identified as polyploid. [Conclusions]Treatment with 0. 5 g /L colchicine for 48 h could well induce the generation of diploidy materials.

    2014年05期 v.5 31-33页 [查看摘要][在线阅读][下载 135K]