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[Objective] The aim was to study on the variation of endogenous hormones contents in Aconitum flavum Hand.-Mazz seeds during the process of dormancy and germination. [Method] Contents of GA3, IAA, ABA and Z in new and old seeds of A. flavum, which was processed under stratification, were determined and analyzed by HPLC, respectively. The germination rate of seeds was investigated. [Result] Content of ABA in seeds declined, while that of GA3 increased during the process of stratification; different stratification treatments had certain effects on seed germination with the extension of time. [Conclusion] GA3 played an important role in breaking dormancy and promoting germination of seeds; ABA was an important inhibitor for germination; IAA played an important role in promoting seeds germination; there was no significant correlation between Z content and seed germination of A. flavum.
2011年07期 v.2 1-4+8页 [查看摘要][在线阅读][下载 379K] [Objective] The aim was to select the appropriate culture medium for the artificial cultivation of FOLIUM PYRROSIAE.[Method] Under the conditions of filed experiment,randomized block design was adopted to study on the active component contents in leaves of FOLIUM PYRROSIAE in four different culture mediums,which were bagasse and soil,mushroom residue and soil,sawdust and soil,slag and soil in the ratio of 1:1.[Result] Bagasse treatment could significantly enhance the contents of total flavonoids,polysaccharides,total saponins,anthraquinone,and chlorogenic acid;sawdust could significantly enhance the contents of chlorogenic acid,polysaccharoses,and total saponins,but had no significant effect on the contents of total flavonoids and anthraquinone.Both mushroom residue and slag treatments could significantly improve the contents of polysaccharides and total saponins,but reduced the content of total flavonoids at the same time,and had no significant effect on the anthraquinone content.[Conclusion] Bagasse treatment was the appropriate culture medium for FOLIUM PYRROSIAE.
2011年07期 v.2 5-8页 [查看摘要][在线阅读][下载 217K] -
[Objective] To study the flowering biomass allocation of Lilium sulphureum Baker apud Hook.f.,so as to provide references for field management and reasonable harvesting of this resource.[Method] The biomasses of different modules of wild Lilium sulphureum Baker apud Hook.f.produced in Wuchuan County of Guizhou Province were quantified.Correlation test and regression analysis on experimental data were carried out by SPSS software.[Result] The order of biomass allocation of single organ was bulb scales>aboveground stems>leaves>flowers>belowground stems>stem roots>scale seats or basal roots.The maximal biomass was invested in bulb scales whether in fresh weight (33.4%) or dry weight (41.2%).The dry weight biomass of bulb scales had extremely significant positive correlation with the basal diameter,plant height and other modules (P<0.01).The dry weight biomass allocation of bulb scales had significant negative correlation with the stem and the propagation module (flower) (0.01<P<0.05).[Conclusion] The growth of a plant was assorted with the nutrition allocation and materiel accumulation.The effect of propagation module and stem on the growth of bulb scales should be controlled in the artificial cultivation of Lilium sulphureum Baker apud Hook.f.
2011年07期 v.2 9-11+15页 [查看摘要][在线阅读][下载 102K] [Objective] To discuss effect of snow melting time on characteristics of growth and phenological phase of Fritillaria unibracteata Hsiao et K.C.Hsia.[Method] In an alpine snow bed on the eastern Tibetan Plateau,the transplant test of F.unibracteata was carried out between early-melting portion and late-melting portion.Individual characteristics and phenological changes of F.unibracteata were observed.[Result] Height,area per leaf and aboveground biomass of F.unibracteata in late-melting portion were significant higher than those in early-melting portion.Snow melting time showed a strong influence on early phenological phases like plant emergence and first flowering,while it presented a weak influence on late phenological phases such as consenescence.[Conclusion] It was more advantageous for F.unibracteata to grow in late-melting habitats than in early-melting locations,which should be followed in artificial cultivation of F.unibracteata on Tibetan Plateau.
2011年07期 v.2 12-15页 [查看摘要][在线阅读][下载 205K] -
[Objective] To study the effect of different growth regulator on raising seedlings of Lycium ruthenicum Murr by hardwood cuttings.[Method] 5 kinds of different growth regulator including 75 mg/L IBA,750 mg/L IAA,750 mg/L GGR7,300 mg/L NAA and 300 mg/L GA3 were adopted in the treatment of cuttings of Lycium ruthenicum Murr.Then,emergence rate,new-shoot number,new-root number and average new-root length of hardwood cuttings were respectively investigated.[Result] The emergence rate of Lycium ruthenicum Murr was influenced mostly by IBA at 85%,followed by IAA (80%),GGR7 (78%),NAA (75%) and GA3 (73%),and the least by clean water at 65%.NAA and GGR7 could enhance the new-shoot number obviously.The new-root number was enhanced very significantly by GGR7,followed by IBA.The average new-root length was enhanced very significantly by GGR7,followed by IAA.[Conclusion] 750 mg/L GGR7 could mostly promote the raising seedlings of Lycium ruthenicum Murr by hardwood cuttings.
2011年07期 v.2 16-17+20页 [查看摘要][在线阅读][下载 80K] [Objective] The aim was to obtain the optimum method to remove protein from polysaccharides of Armillariella tabescens.[Method] Deproteinization from the polysaccharides of A.tabescens was conducted by Sevag method,enzyme method,trichloroacetic acid(TCA)method;and the combination of enzyme method and Sevag method;and the optimum deproteinization method was selected.[Result] The combination of enzyme method and Sevag method was better than others with the contents of protein being only 0.18%;the optimum condition for enzyme method was 0.5% enzyme dosage,40 ℃,water bath for 0.5 h and pH=6.[Conclusion] The combination of enzyme method and Sevag method was the optimum method for deproteinization.
2011年07期 v.2 18-20页 [查看摘要][在线阅读][下载 113K] -
[Objective] The aim was to determine the contents of metallic elements in Indigofera fortunei Craib from Mengzi,Yunnan Province,so as to provide scientific basis for its further development and utilization.[Method] Contents of six metallic elements including Ca,Fe,Zn,Cu,Pb and Cr in I.fortunei were determined by flame atomic absorption spectrometry.[Result]I.fortunei from Mengzi,Yunnan Province was rich in Ca,Zn,Cu and Fe.The contents of four metallic elements were in the order of Ca > Fe > Zn > Cu.Harmful metallic elements Pb and Cd were not detected in tested samples.[Conclusion]I.fortunei was rich in metallic elements.
2011年07期 v.2 21-22页 [查看摘要][在线阅读][下载 71K] [Objective] The aim was to determine the content of eight metal elements in Typha latifolia L.in Jianshui County,Yunnan Province,China,so as to provide a scientific basis for the development and utilization of this.[Method] Contents of eight metal elements,including Fe,Zn,Cu,Ni,Co,Cd,Pb and Cr were determined by flame atomic absorption spectrometry.[Result] There were abundant mineral elements in T.latifolia which were beneficial to human.Their contents were in the order of Fe>Zn>Cu>Ni>Co>Cd>Pb and element Cr was not detected.The content of Fe was high in T.latifolia,while Cu was relatively low.[Conclusion] There were abundant mineral elements which were necessary for human body in the T.latifolia.
2011年07期 v.2 23-24页 [查看摘要][在线阅读][下载 71K] -
[Objective] To analyze the volatile components from Sabina procumbens (Endl.) Iwata et kusaka.[Method] The volatile components from Sabina procumbens (Endl.) Iwata et kusaka were firstly extracted by using simultaneous distillation,and then analyzed and identified by gas chromatography-mss spectrometry (GC-MS) and computer spectrum searching.[Result] 0.68% of the volatile oil was extracted from the Sabina procumbens (Endl.) Iwata et kusaka.88 compounds were identified occupying 85.74% of the total extracts,among which the main compositions were β-phellandrene (17.06%),terpinen-4-ol (2.96%),β-citronellol (2.34%),2-undecanone (8.35%),6-octenoic acid,3,7-dimethyl-,methyl ester (5.87%) and α-cadinol (4.07%).[Conclusion] The study on the volatile components from fresh leaves of Sabina procumbens (Endl.) Iwata et kusaka could provide a test basis for further development and utilization of this resources.
2011年07期 v.2 25-27+38页 [查看摘要][在线阅读][下载 118K] [Objective] To study the chemical components of volatile oils from leaves of Uvaria microcarpa Champ.ex Benth.[Method] Volatile oils were extracted from leaves of Uvaria microcarpa Champ.ex Benth by supercritical fluid CO2 extraction (SFE-CO2),and then their chemical components were separated and identified by GC-MS.[Result] 58 chromatographic peaks were isolated from leaves of Uvaria microcarpa Champ.ex Benth,among which 37 compounds were identified occupying 89.70% of the total volatile oils,mainly referred to linoleic acid,palmitic acid,mono (2-ethylhexyl) phthalate,bicyclogermacrene and benzoic acid.[Conclusion] There were obvious differences in chemical components of volatile oils extracted by SFE-CO2 and steam distillation.
2011年07期 v.2 28-29+41页 [查看摘要][在线阅读][下载 112K] -
[Objective] To establish methods for determining contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing,so as to provide a scientific basis for further development and utilization of this resources and quality control.[Method] Contents of total flavonoids and rutin were respectively determined by aluminium trichloride colorimetric method and HPLC.[Result] Contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing were 5.485-5.742 and 0.769-0.811 mg/g,respectively.[Conclusion] The method was simple,rapid,accurate,stable and suitable for determining the content of total flavonoids and rutin in Ephorbia helioscopia L..
2011年07期 v.2 30-32+45页 [查看摘要][在线阅读][下载 178K] [Objective] To compare the contents of volatile oil and germacrone in RHIZOMA CURCUMAE from different producing area such as Lingshan,Guiping and Guigang in Guangxi Province.[Method] The volatile oil was extracted from RHIZOMA CURCUMAE by steam distillation.The content of germacrone was determined by high performance liquid chromatography method (HPLC),and its chromatograph conditions were as follows:acetonitrile-water (V/V,70:30),flow rate of 1 ml/min,column temperature of 35 ℃,detection wavelength of 215 nm and sampling volume of 10 μl.[Result] The germacrone presented a good linear relation between the detection concentration of 0.022 3 and 0.139 8 mg/ml.Contents of volatile oil and germacrone in RHIZOMA CURCUMAE were varied for different producing area.[Conclusion] The contents of volatile oil and germacrone in RHIZOMA CURCUMAE from Lingshan were higher than that in other 8 areas.
2011年07期 v.2 33-34+49页 [查看摘要][在线阅读][下载 1278K] -
[Objective] To optimize the extraction technology of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method.[Method] Effects of enzymolysis temperature,ethanol volume fraction,extraction time and volume of extracting solvent on the extraction of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method were investigated through single factor test and quadratic regression rotation-orthogonal combination design.[Result] The optimum extraction conditions were as follows:enzymolysis temperature of 10 ℃,ethanol volume fraction of 40%,extraction time of 60 min,and volume of extracting solvent of 70 ml.The theoretical extraction rate of chlorophyll was 32.29 mg/g which was approximated to the experimental value of 31.87 mg/g.[Conclusion] This study could provide reference for rational development and utilization of Brassica juncea var.tumida Tsen et Lee and improvement of extraction rate of chlorophyll.
2011年07期 v.2 35-38页 [查看摘要][在线阅读][下载 157K] [Objective] To obtain a suitable macroporous adsorption resin and optimize its purification process of total flavonoids from root of Rosa laevigata Michx..[Method] Effect of 4 resins like ADS-7,HPD300,AB-8 and D-101 on the adsorption and desorption of total flavonoids from root of Rosa laevigata Michx.were comparatively studied,and effects of concentration of loading solution and eluent concentration on desorption performance were investigated.[Result] AB-8 was the optimum macroporous adsorption resin for purifying total flavonoids from root of Rosa laevigata Michx..The optimum process to isolate and purify total flavonoids was that 20.0 ml of 0.200 mg/ml sample solution was adsorbed on the surface of AD-8 resin and eluted with 4 BV of 50% ethanol.Under the optimum conditions,the purification rate of total flavonoids from root of Rosa laevigata Michx.reached as high as 205.40%.[Conclusion] The high quality total flavonoids from root of Rosa laevigata Michx.were obtained in this work.
2011年07期 v.2 39-41页 [查看摘要][在线阅读][下载 118K] -
[Objective] The aim was to prepare sustained-release microcapsules of baicalin,as well as to design and optimize the prescription,and to make initial quality standards for this preparation.[Method] Complex coacervation method was applied to prepare sustained-release microcapsules using gelatin and Arabic gum as capsule materials,baicalin capsule as core and Tween-80 as the wetting agent.Taking the entrapment efficiency as primary quality index,the preparation process of microcapsules was optimized by single factor experiment and orthogonal test.Morphology of microcapsule was observed using microscopy and electron microscopy;content of microcapsule was determined by ultraviolet spectrophotometry;the in vitro dissolution of this preparation was investigated using water as dissolution medium.[Result] The optimum prescription for baicalin microcapsules was:13.3 mg/ml gelatin and Arabic gum,50 ℃ reaction temperature,pH 3.90,and material-drug ratio 5:1.Microcapsule obtained by the optimum prescription had good dispersion,and significant loading rate.The morphology was round and smooth with approximately the same size.[Conclusion] Baicalin sustained-release microcapsules with high entrapment efficiency could be successfully prepared by complex coacervation method,and the product had good stability.This method was feasible,reproducible,and of some practical value.
2011年07期 v.2 42-45页 [查看摘要][在线阅读][下载 140K] [Objective] This study was to investigate the effects of fermentation time on the contents of two main toxic ingredients of fatty oil and total protein in Lingba Junzhi (LBJZ,a croton product fermented by Ganoderma/ucidum (Curtis:Fr.)P.Karst.).[Method] Using gravimetric analysis and Coomassie brilliant blue G-250 staining method,the contents of fatty oil and total protein in LBJZ fermented at day 0,12,16,20,23,26,29 and 32 were determined and compared,respectively.After methyl esterification,the components of fatty oils were further analyzed quantitatively and qualitatively by GC-MS,the relative contents of which in percentage were determined with area normalization method.[Pesult] The contents of both fatty oil and total protein in LBJZ decreased on the whole with prolonged fermentation time.The minimum from day 23 to 32 and from day 12 to 32 of fermentation respectively,and retained at a rather stable level.Forty two chemical compounds in total were isolated and identified from fatty oils by GC-MS during the whole fermentation process,among which 11 compounds had the relative contents more than 1.0%.Compared with the fermentation at day 0,contents of 6 compounds increased while 5 compounds decreased on the 32nd day of fermentation,and at least 8 novel substances were formed.[Conclusion] The optimal fermentation time of LBJZ is around 23 to 26 days.
2011年07期 v.2 46-49页 [查看摘要][在线阅读][下载 3931K] -
[Objective] To investigate the optimum extraction technology of total flavonoids from celery leaves by using Soxhlet extraction method,so as to provide theoretical basis for development and utilization of celery leaves.[Method] With the ethanol as solvent,Soxhlet method was adopted to extract total flavonoids from celery leaves.Effects of ethanol concentration,extraction time,extraction temperature and solid-liquid ratio on the yield of total flavonoids were respectively investigated through the single-factor test.[Result] Factors that affected the yield of total flavonoids were in the order of ethanol concentration>solid-liquid ratio>extraction temperature>extraction time.The optimum technology conditions of total flavonoids from celery leaves by using Soxhlet extraction method were as follows:ethanol concentration of 60%,extraction time of 1 h,extraction temperature of 70 ℃,and solid-liquid ratio of 1:60 g/ml.Under the optimum condition,the yield of total flavonoids was 47.85 mg/g.[Conclusion] The Soxhlet extraction method was available for extracting total flavonoids from celery leaves.
2011年07期 v.2 50-52页 [查看摘要][在线阅读][下载 146K] [Objective] To establish a method for distinguishing RADIX GINSENG from its counterfeits including RADIX PLATYCODI,RADIX ISATIDIS and RADIX GLEHNIAE by ultraviolet absorption spectra lines group (UASLG).[Method] The 4 kinds of medicines were respectively extracted with five different solvents of water,ethanol,petroleum ether,ethyl acetate and chloroform.Then,their absorbance in UV and one-order derivative spectra was measured.[Result] There were obvious differences regarding peak position and absorbance in ultraviolet absorption spectra among RADIX GINSENG and RADIX PLATYCODI,RADIX ISATIDIS,RADIX GLEHNIAE.[Conclusion]The method is simple and accurate,and could be used to distinguish RADIX GINSENG from its counterfeits.
2011年07期 v.2 53-56+59页 [查看摘要][在线阅读][下载 317K] -
[Objective] To determine the optimum extraction conditions of polysaccharides from Camellia chrysantha (Hu) Tuyama by acetone deposition method.[Method] Extracts of polysaccharides that were obtained from Camellia chrysantha (Hu) Tuyama as research object and water as solvent under certain conditions were precipitated with acetone.Using the amount of acetone,precipitation time and centrifugation time as investigated factors,single factor and orthogonal test were carried out to optimize the extraction process of polysaccharides from Camellia chrysantha (Hu) Tuyama.[Result] The optimum extraction conditions of polysaccharides from Camellia chrysantha (Hu) Tuyama by acetone deposition method were obtained:centrifugation time of 15 min,acetone amount of 20 ml and precipitation time of 12 h.Under the above condition,the weight of polysaccharides was 0.003 7 g.[Conclusion] The method established in this study was simple,quick and practical.
2011年07期 v.2 57-59页 [查看摘要][在线阅读][下载 121K] [Objective] To optimize the preparation process of milk beverage with RHIZOMA DIOSCOREAE and SEMEN ARMENIACAE AMARUM as raw materials.[Method] Detoxification and debittering conditions of SEMEN ARMENIACAE AMARUM were confirmed.Effects of different emulsion stabilizer on the stability of milk beverage were investigated.The preparation process of milk beverage with SEMEN ARMENIACAE AMARUM and RHIZOMA DIOSCOREAE was optimized by orthogonal experiment.[Result] The emulsion stabilizer including 0.08% of monoglyceride,0.02% of sucrose ester,0.07% of polyglycerin ester,0.03% of sodium caseinate,0.08% of CMC,0.02% sodium alginate,0.03% of xanthan gum and 0.07% of pectin was the optimum formula determined in this study.The optimum preparation formula of milk beverage was as follows:30.00% of SEMEN ARMENIACAE AMARUM milk,15.00% of RHIZOMA DIOSCOREAE juice,pH of 3.8 and 10.00%of white granulated sugar.[Conclusion] The milk beverage prepared by this process was good in color and flavor,and stable in state.
2011年07期 v.2 60-62+66页 [查看摘要][在线阅读][下载 108K] -
[Objective] The aim was to find out the optimum conditions for separation and purification of total saponins from RADIX ASTRAGALI.[Method] Employing ultraviolet spectrophotometry as dynamic adsorption-desorption method,the content of total saponins in RADIX ASTRAGALI was determined to reveal the optimum conditions for separating and purifying total saponins from RADIX ASTRAGALI with macroporous resin.[Result] The optimum conditions for separation and purification of total saponins with AB-8 were as follows:extract concentration of 0.1 mg/ml,sampling volume of 3 BV,flow rate of 1.0 ml/min,washing with 4 BV of distilled water first and then desorption with 4 BV of 50% ethanol.Under these conditions,the yield of total saponins was reached as high as 52.1%.[Conclusion] This method is simple and feasible,which could meet industrial requirements.
2011年07期 v.2 63-66页 [查看摘要][在线阅读][下载 179K] [Objective] To optimize the extraction technology of paeonol from CORTEX MOUTAN.[Method] Taking extraction rate/yield of paeonol as index,effects of ultrasonic temperature,ultrasonic time,extracting solvent concentration (NaCl concentration) and solid-liquid ratio (NaCl dosage) on extraction rate/yield of paeonol from CORTEX MOUTAN were investigated by L9 (34) orthogonal test.[Result] The optimum extraction technology of paeonol was as follows:ultrasonic temperature of 50 ℃,ultrasonic time of 20 min,NaCl concentration of 3% and solid-liquid ratio of 1:10 g/ml.Under this condition,the extraction yield and rate of paeonol were 0.964 0 g and 85.69%,respectively.[Conclusion] Ultrasonic wave had obvious effects on extraction rate/yield of paeonol from CORTEX MOUTAN.
2011年07期 v.2 67-68+71页 [查看摘要][在线阅读][下载 87K] -
[Objective] To screen out the optimal medium for tissue culture of wild Lilium lancifolium.[Method]The tissue culture of wild Lilium lancifolium was studied using its scales,bulbils,stem pieces and leaves as explants respectively.The basic mediums were MS,B5 and White that included 0.03 mg/L of NAA.Four differential mediums were:MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10 mg/L KT+0.10 mg/L NAA and MS+0.03 mg/L NAA.Six proliferation formulas were as below:MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10 mg/L KT,MS+0.20 mg/L KT+0.20 mg/L NAA and MS+0.10 mg/L KT+0.15 mg/L NAA.Four rooting media were:MS+1.00 mg/L IBA,MS+1.00 mg/L IBA+0.20 mg/L 6-BA,1/2MS+1.00 mg/L IBA+0.20 mg/L 6-BA and White+1.00 mg/L IBA+0.20 mg/L 6-BA.[Result] MS medium was the optimum basic medium.The optimal differential mediums for scale,bulbils,leaf and stem pieces of wild Lilium lancifolium were MS+0.03 mg/L NAA,MS+2.00 mg/L 6-BA+0.20 mg/L NAA,MS+0.20 mg/L IAA,and MS+2.00 mg/L 6-BA+0.20 mg/L NAA,respectively.The optimum subculture medium was MS+ 0.10 mg/L KT +0.15 mg/L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1/2MS+0.20 mg/L 6-BA+1.00 mg/L IBA.In the field,the tissue culture seedlings formed by scales and bulbils grew more strongly than others.[Conclusion] The optimal tissue culture mediums for wild Lilium lancifolium were obtained in this work,and this study provided basis for the rapid propagation of wild Lilium lancifolium.
2011年07期 v.2 69-71页 [查看摘要][在线阅读][下载 84K] 下载本期数据