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[Objective] This research was to study the effect of palygorskite adding NPK fertilizer on plant dry matter accumulation and polysaccharide content of Radix Hedysari,providing a scientific basis for the rational fertilization of Radix Hedysari.[Method] Plant dry matter accumulation of Radix Hedysari of growth period in each sample was determined;the yields after post-harvest were calculated;the polysaccharide content of Radix Hedysari was determined with phenol-sulfuric acid method and the polysaccharide yields were calculated.[Result] Palygorskite and NPK fertilizer can promote the dry matter accumulation and the HPS content of the underground sections at the latter period (the latter September and the end October) of Radix Hedysari,or slow down the reduction of polysaccharide content and improve the quality of Radix Hedysari;and can significantly increase the yield of Radix Hedysari and polysaccharide.The high-volume palygorskite(2 250 kg/hm2) and NPK fertilizers can significantly increase the yield of Radix Hedysari and polysaccharide,to 731.32 kg/hm2.The single application of Palygorskite can promote the amount of dry matter accumulation and the polysaccharide content of various parts of Radix Hedysari,and slow down the reduction of polysaccharide content of underground parts and promote the yield of Radix Hedysari and polysaccharide.The effect of high-volume palygorskite(2 250 kg/hm2) and NPK fertilizers was obvious,increasing to 368.34 kg/hm2.[Conclusions] Palygorskite and NPK fertilizer can increase the use of NPK fertilizer and significantly increase the yield and quality of R.Hedysari,the effect of the high-volume palygorskite(2 250 kg/hm2) and NPK fertilizer was obvious.The single application of palygorskite had the effect of extending the period of dry matter accumulation and delaying the polysaccharide content reduction.
2010年04期 v.1 1-4+7页 [查看摘要][在线阅读][下载 386K] <正>1.Requirement for ArticlesThe journal mainly publishes original research papers,research notes,letters and reports about new technology on pharmaceutics,medicinal botany,medicinal chemistry of natural
2010年04期 v.1 2页 [查看摘要][在线阅读][下载 242K] -
<正>Sir/Madam:Shalom! In order to expand the spreading scale of medicinal plant researching,increase the international academic exchange opportunities and improve the international influence on the protection,development and utilization of
2010年04期 v.1 4页 [查看摘要][在线阅读][下载 33K] [Objective] The aim was to optimize the extraction condition of tanshinone Ⅱ A from Salvia miltiorrhiza Buhge.with ultrasonic extraction method.[Method] On the basis of single factor experiment,an optimum extracting technology of tanshinone Ⅱ A from S.miltiorrhiza was investigated through the orthogonal experiment under the ultrasonic wave assistance conditions,then the content of tanshinone Ⅱ A from S.miltiorrhiza in extract was analyzed with HPLC method.[Result] The optimum extraction conditions were as follows:at room temperature,ethanol volume fraction 85%,extraction time 30min,solid-liquid ratio 1:10,for four times.Under the above conditions,the content of tanshinone Ⅱ A in extract was 0.388%.[Conclusion] Extracting tanshinone Ⅱ A with ultrasonic extraction method has the following characteristics:rapid,efficient,convenient and low energy consumption.It is beneficial to produce tanshinone Ⅱ A in large scale.
2010年04期 v.1 5-7页 [查看摘要][在线阅读][下载 138K] -
[Objective] The aim was to screen out the differentiation medium formula for the virus-free test-tube seedling from stem tips of Dioscorea oppasita.[Method] With MS as the basic medium,a three-factor and three-level test was designed with 6-benzyl aminopurine (6-BA),2,4-dichlorophenoxyacetic acid (2,4-D) and naphthylacetic acid (NAA) as treatment factors and different hormones concentrations as levels.The screening of the differentiation medium for virus-free seedling from the stem tips of D.oppasita was studied.[Result] Formula of MS+2mg/L 6-BA+1mg/L 2,4-D+ 3mg/L NAA was the optimum differentiation medium for the virus-free seedling of D.oppasita.[Conclusion] D.oppasita cultured in the screened medium could reach a favorable balance between dedifferentiation and differentiation of stem tips.The medium is a suitable differentiation medium for culturing the virus-free seedlings of D.oppasita stem tips.
2010年04期 v.1 8-9+12页 [查看摘要][在线阅读][下载 145K] [Objective] The research aimed to study the Juglans regia and Arachis hypogaea by Fourier transform infrared spectroscopy (FTIR).[Method] The infrared spectra of Arachis hypogaea and Juglans regia from six different producing areas were analyzed.[Result] The results showed that the spectral shape and position of the peaks were very similar for the Juglans regia from different areas and between the Juglans regia and the Arachis hypogaea.The differences were observed among the absorbance ratios of several peaks.For the Juglans regia and Arachis hypogaea,the absorbance ratio A1746/A1653 was greater than 1,while A1099/A1653 was slightly larger than 1 for the Juglans regia and less than 1 for the Arachis hypogaea.By the differences of absorbance ratios of A1746/A1653 and A1099/A1653,Juglans regia from six different areas might be discriminated.[Conclusion] The FTIR might be a rapid,convenient simple and non-destructive method in determination of the origin of agricultural products.
2010年04期 v.1 10-12页 [查看摘要][在线阅读][下载 147K] -
[Objective] The research aimed to establish the propagation system of tissue culture seedlings of Gentiana scabra Bunge.[Method] Using the tender stems of G.scabra as materials,the callus induction and differentiation,the differentiation and rooting of adventitious buds,the transplanting of tube seedlings were studied.[Result] The ideal medium for inducing the tender stems to form the differentiation calli was MS+ 0.2mg/L BA+ 1.0-1.5mg/L 2,4-D.The ideal medium for the differentiation culture of calli and adventitious buds was MS 1.2mg/L AgNO3+ 0.6mg/L BA+ 0.1mg/L NAA.The ideal medium for the rooting culture and rooting sub-culture of G.scabra tube seedling was 1/2 MS+ 0.1mg/L IAA+ 0.3mg/L NAA.The botanic characters of G.scabra tube seedlings transplanted on the hillside were remained.[Conclusion] Under these experimental conditions,the transplanting survival ratio of cultured tube seedlings reached 96%,indicating that the method can be used in field production completely.
2010年04期 v.1 13-15+23页 [查看摘要][在线阅读][下载 104K] The effective chemical compositions and nutrient components in medicinal and edible homogeny wilding Rosa laevigata Mickx.were briefly described.The research and development situations of main bioactive components in the extraction of Rosa laevigata were summarized.The existing problems and further research directions were proposed so as to provide theory basis for the medicinal research and comprehensive utilization of Rosa laevigata Mickx..
2010年04期 v.1 16-23页 [查看摘要][在线阅读][下载 183K] -
[Objective] Flame atomic absorption spectrometry was used to determine the levels of elements content in Asparagus officinalis L.,which could provide references for the rational utilization and development of A.officinalis L.[Method] A.officinalis L.samples were digested using nitric acid and hydrogen peroxide as solvents in high-pressure closed-vessel.LaCl3 and CsCl were used as releasing agent to eliminate the chemical interference to calcium,magnesium and potassium determination.[Result] The rate of recovery varied from 91.2% to 112.3%,and the relatively standard deviation was from 1.43% to 2.17%.The copper,iron,zinc and calcium contents of A.officinalis L.cans were higher than those before processing,but their potassium and magnesium content were lower than those before processing.The copper,iron,zinc,calcium,magnesium and potassium contents of green A.officinalis L.were higher than those contained in white Asparagus offcinalis and utilization.
2010年04期 v.1 24-26页 [查看摘要][在线阅读][下载 87K] [Objective]The research aimed to establish the detection method of isoorientin from different parts of Gentianna straminea Maxim.by reversed phase-HPLC.[Method]The chromatographic conditions were as follows:Hypersil ODS column (4.6 mm×250 mm,5μm),40% methanol-H2O (containing 0.02% phosphoric acid) as a mobile phase,with flow rate of 1 ml/min,column temperature at 35℃,detection wavelength at 254 nm.[Result]Under the chromatographic conditions,isoorientin and other components reached the baseline separation.The linear range was 0.089 9-0.539 μg,r=0.999 9.The addition recovery was 99.7%,with RSD of 0.49%.[Conclusion]The method is rapid and accurate and the results are reliable.
2010年04期 v.1 27-28+32页 [查看摘要][在线阅读][下载 114K] -
[Objective] The aim was to study the extraction technological conditions of total flavonoids from Toona sinensis leaves in order to provide theoretical basis for further development of T.sinensis leaves in the field of food and medicine.[Method] Spectrophotometry was adopted to determine the total flavonoids in T.sinensis leaves.By using single-factor test,the effects of ethanol concentration,solid-liquid ratio,temperature and time on extraction rate of the total flavonoids were analyzed.Based on this,the extraction technological conditions of the total foavonoids from T.sinensis leaves were optimized through orthogonal design method.[Result] The results showed that the optimal extracting conditions were as follows:ethanol concentration 70%,solid-liquid ratio 1:50,extracting duration 2.0 h and refluxing temperature 70℃.Under these optimum experimental conditions,the total flavonoids yield from T.sinensis leaves could reach 5.84%.[Conclusion] The test was repeated for 5 times under the optimum extraction conditions,and the mean yield of total flavonoids was 5.84%.Therefore,the method is stable and reliable.
2010年04期 v.1 29-32页 [查看摘要][在线阅读][下载 99K] [Objective] To investigate the chemical compositions from the cortex of Broussonetia papyrifera.[Method] The cortices of Broussonetia papyrifera were extracted with 95% ethanol.The EtOH extract was dispersed in H2O and extracted with petroleum,EtOAc and BuOH successively.The EtOAc fraction was isolated and purified by column chromatography on silica gel and Sephadex.The compounds were identified on the basis of spectral analysis (including MS,1H-NMR,1C-NMR).[Result] Nine compounds were isolated from the cortex of Broussonetia papyrifera,and its structure was characterized as kazinol B(1),7,4'-dihydroxy-3'-prenylflavan(2),7,3'-dihydroxy-4'-methoxyflavan(3),kazinol A (4),3'-(3-methylbut-2-enyl)-3',4',7-trihydroxyflavane(5),Broussochalcone A (6),oleanolic acid (7),2',3β-dihydroxy-12-oleanen-28-oic acid (80),daucosterol (9).[Conclusion] 3,7,8 were obtained from the cortex of Broussonetia papyrifera for the first time.The antibacterial test showed that compound 1,2,5,7 had better inhibition on S.m ATCC25175,A.n ATCC12104,P.g ATCC33277 and F.n ATCC10953.
2010年04期 v.1 33-35页 [查看摘要][在线阅读][下载 98K] -
[Objective] This research was to study the protective effects of phenolic acid from Salvia chinensis Benth.(PAS) against CCL4-induced acute liver injury in mice and its possible mechanism.[Method] Mice were divided into control group,model group randomly,S.chinensis was divided into 6 groups such as high,medium and low-dose treatment group and positive group(Silibinin).Mice were pretreated by phenolic acid(i.g) for 5 d.Hepatic injury was induced by injecting 1 ml/kg 50% peanut oil solution of carbon tetrachloride,the contents of AST,ALT,T-AOC,MDA,GSH were determined after 24.[Result] Compared with model group,serum AST,ALT in PAS high,medium and low-dose groups reduced significantly;total antioxidant capacity(T-AOC),reduced gluthione(GSH) increased,as well as MDA content decreased significantly,all above indexes changed in a dose-dependent way.Histopathological study showed that the protective effects of the PSA also indicated a dose-dependent manner.[Conclusion] The PAS has a protective effect on the carbon tetrachloride induced acute liver injury in mice,hepatoprotective effects of PAS may be related to its antioxidant characteristic.Total phenolic acids of S.chinensis can enhance anti-oxidant capacity of organizations,reduce carbon tetrachloride induced lipid peroxidation,and protect cell membranes from damage.
2010年04期 v.1 36-38+41页 [查看摘要][在线阅读][下载 365K] [Objective] To identify the characteristics of nrDNA-ITS from the leaf of famous-region drug Codonopsis tangshen Oliv.It will provide molecular evidence to distinguish C.tangshen Oliv.[Method] Direct PCR sequencing was used to detect the homology of nrDNA-ITS sequence of C.tangshen Oliv and analyze its sequence composition.[Result] The ratio of OD260/OD280 of genome DNA absorption value in samples extracted by improved CTAB was 1.8-2.0,DNA strips were clear and tidy,no obvious tail phenomenon,indicating that purity and quality of DNA samples was better,met the test requirements.NrDNA-ITS sequences universal primers were used to PCR amplification reactions,the expected double-stranded product of about 800 bp were obtained.After sequencing,it revealed that the Nucleotide sequence length of C.tangshen Oliv.nrDNA-ITS1 sequence was 260 bp,its G+C contents were 52%,the nucleotide sequence length of nrDNA-ITS sequence,and had been submitted to GenBank,registration No.was GQ906566.[Conclusion] DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of C.tangshen Oliv,which can also provide the basic information for molecular identification of C.tangshen Oliv.
2010年04期 v.1 39-41页 [查看摘要][在线阅读][下载 154K] -
[Objective] The research aimed to determine the optimum extraction technology of volatile oil from Rhododendron capitatum.[Method] Three factors of extraction time (A),soaking time (B),water-adding multiple (C) were selected with three levels in each factor.Using the orthogonal test,taking the extraction amount of volatile oil as the observation index,the best conditions of water steam distillation method were screened out.[Result] The influence ranking of three factors on the extraction process of volatile oil was as follows:extraction time>soaking time> water-adding multiple.The optimum combination was A2B2C1,that was to say,adding water ten times,extraction time of 3 h,soaking time of 2 h.Because water addition had little effect on the extraction amount of volatile oil,it was determined as 1:8 so as to save the cost.So the optimum extraction process was determined as A2B2C1.The verification test was carried under these conditions.The extraction amount of volatile oil was 1.5% with RSD=0.066%,which indicated that the optimized method was accurate and reliable.[Conclusion] The optimized extraction method is easy to control and it has simple instrumentation.The research provides a theoretical basis for the production and wide use of Rhododendron capitatum.
2010年04期 v.1 42-43+46页 [查看摘要][在线阅读][下载 167K] By applying the principal component analysis and cluster analysis method,combining with the statistic software SPSS,the comprehensive evaluation of quantitative and qualitative of trace elements in Nitraria leaf from Qinghai region were studied and a new area in comprehensive evaluation scientifically was explored.
2010年04期 v.1 44-46页 [查看摘要][在线阅读][下载 116K] -
[Objective] The research aimed to optimize the best extraction technology of ferulic acid in Angelica sinensis(Oliv.) Diels.[Method] The active ingredients in A.sinensis were extracted by using supercritical CO2 extraction technology.The effects of particle size,extraction pressure,extraction temperature,separation temperature on the extraction rate of volatile oil and ferulic acid were investigated.And their contents were determined by using high performance liquid chromatogram.[Result] The optimized technology parameter of extracting volatile oil from A.sinensis by using supercritical CO2 extraction technology were as follows:the particle size of 30,extraction pressure of 28MPa,extraction temperature of 35℃,separation temperature of 45℃ and the separation pressure of 6MPa.The optimized technology parameter of extracting ferulic acid from A.sinensis by using supercritical CO2 extraction technology were as follows:the particle size of 10,the extraction pressure of 22MPa,extraction temperature of 35℃,separation temperature of 40℃ and the separation pressure of 6MPa.CO2 flow was 20 L/h and the extraction time was 2 h.[Conclusion] The optimized process of the active ingredients in A.sinensis had higher extraction rate and the efficacy components were retained better.
2010年04期 v.1 47-50页 [查看摘要][在线阅读][下载 191K] [Object]To investigate the purification conditions of total flavonoids from Aurantii immaturus Fructus by macroporous resin.[Method] The adsorption quantity and desorption rate of three kinds of macroporous resins (AB-8,HPD-450,D101) were compared;the separation parameters of D101 on total flavonoids were studied.[Results] D101 is the best macroporous resin on the separation of total flavonoids from Fructus Aurantii immaturus.The optimal adsorption conditions are as follows:3 mg/ml of total flavonoids concentration,sample volume is 2 times of the bed volume (BV),pH 4.5,70% ethanol as eluent and elution volume is 2 times of BV.[Conclusion] D101 macroreticular resin showed better comprehensive adsorption property and can be used to separate and purify the total flavonoids in Aurantii immaturus Fructus.
2010年04期 v.1 51-53+57页 [查看摘要][在线阅读][下载 198K] -
[Objective]To establish an effective method for controlling the quality of Astragalus polysaccharide Meal.[Method]The assay of glucose and sucrose in the self-made Astragalus polysaccharide meal was semi-quantitatively determined by TLC and the assay of total sugar was determined by phenol-sulfuric acid method.The influencing factors of measuring repeatability were preliminarily studied.[Results]The assay of glucose,sucrose and total sugar in the Astragalus polysaccharide meal were 0.83%,6.67%,42.0% respectively.[Conclusion]The methods in this research were simple and rapid,and can be used as a site detection technique for Astragalus polysaccharide preparations.
2010年04期 v.1 54-57页 [查看摘要][在线阅读][下载 191K] [Objective] The aim was to establish the activity assay system of uridine 5'-diphosphoglucose(UDPG):ginsenoside Rh2 glucosyltransferase(UGRh2GT) of Panax ginseng cells.[Method] With UDPG as the glucose donor,ginsenoside Rh2 was catalyzed into Rg3 by certain enzyme which was extracted from P.ginseng suspension cells at appropriate temperature and pH.The generated ginsenoside Rg3 was determined by high performance liquid chromatography (HPLC).The analytical column was C18 (150 mm×4.6 mm).Mobile phases were acetonitrile and water with gradient elution.[Result] In the activity assay system of UGRh2GT of ginseng cells,the optimum pH value was 9.3,the optimal temperature was 34.1℃.[Conclusion] The method of activity assay can determine the activity of UGRh2GT more easily.
2010年04期 v.1 58-60页 [查看摘要][在线阅读][下载 231K] -
[Objective] The research aimed to discuss the dormancy mechanism of Panax quinquefolium Linne.seeds.[Method] The germination inhibitors were extracted and isolated by using systematic solvent extraction,separated by TLC.The germination inhibitors were purified by using GC-MS and the structural formula was identified.[Result] 21 kinds of germination inhibitors were identified in the pulp of Panax quinquefolium Linne..[Conclusion] It was proved that the pulp of Panax quinquefolium Linne.contained many germination inhibitors and the inhibition activity showed a reserve relationship with the dilution times.
2010年04期 v.1 61-63页 [查看摘要][在线阅读][下载 133K] 下载本期数据