-
[Objective] The paper aimed to study the rapid propagation method of Spatholobus suberectu, which provided a reference and technical basis for its cultivating. [Method] Root stimulate Fengyebao, IBA, rooting powder ABTI were chosen as chemical enhancers of cutting slips; three different cutting types with different lignification degrees were chosen; Sand, mud rock and perlite were chosen for the study on the optimization of cutting propagation techniques of Spatholobus suberectus with single-factor test. [Result] The results showed that the optimum conditions of rooting effects as follows, dipping the lignified cutting kept 2-3 nodes in ABTI 300 mg/L for 2 hours and cutting in pearlite or yellow soil in spring. [Conclusions] The feasibility of cutting propagation of Spatholobus suberectus was good.
2010年03期 v.1 1-3页 [查看摘要][在线阅读][下载 95K] <正>1. Requirement for Articles The journal mainly publishes original research papers, research notes, letters and reports about new technology on pharmaceutics,medicinal botany, medicinal chemistry of natural products, pharmacology and toxicology, and other related fields. These articles can refer to basic theory, applied research and high-tech, etc. A manuscript must offer detailed document and reliable data. The innovative
2010年03期 v.1 2页 [查看摘要][在线阅读][下载 254K] -
[Object] The study was to provide a reference for organ development and molecular study. [Method] Fritillaria ussuriensis Maxim. was used as an experiment material, total soluble sugar, reducing sugar and starch contents of tissue culture as indexes, the metabolism of carbohydrates in cultures of Fritllaria ussuriensis Maxim. during different stages of tissue culture were studied. [Result] The total soluble sugar and reducing sugar contents showed decrease and then increase trend in cultures of Fritillaria ussuriensis during different stages, and reached their maximum of 53.41 and 9.23 mg/g at a bulblet growing stage (bulblet-2). Total starch contents showed increase-decrease-increase trend in cultures. At callus stage reached their minimum of 42.32 mg/g, and reached maximum of 84.06 mg/g at a bulblet expanding stage (bulblet-2). [Conclusion] The mechanism of the metabolism of carbohydrates was verified, which provided a theoretical basis for organ development.
2010年03期 v.1 4-5+8页 [查看摘要][在线阅读][下载 232K] [Objective]The purpose was to find out the most suitable growth condition region of Cynomorium Songaricum.in XinJiang and provide a reference for standardizing planting, site selection and rational planning of scientific production layout of Cynomorium Songaricum. [Method] On the basis of field survey sampling point, the specimen inspection and literature,comprehensive analyzed by Traditional Chinese Medicine Geographic Information System-Ⅰ. [Result]The results of origin suitable area analysis showed that suitable area >90% including 70 counties and cites and accounting for 70.7% in XinJiang. Suitable plant areas were 198 276 km2, accounting for 12.4% in XinJiang. [Conclusion]Cynomorium Songaricum distributed widely in XinJiang province, almost can be planted in whole XinJiang.
2010年03期 v.1 6-8页 [查看摘要][在线阅读][下载 172K] -
[Objective]The research aimed to optimize the extraction methods for genomic DNA in Eomecon chinantha Hance.. [Method]The methods of CTAB and modified CTAB were chosen to extract the genomic DNA in Eomecon chinantha Hance. leaves.[Result]The results showed that the genomic DNA extracted by CTAB method contained many impurities, which were difficult to dissolve and easily degradable.The modified CTAB method was suitable to extract the genomic DNA in Eomecon chinantha Hance..The concentration and extraction rate of DNA from both fresh leaves and dry leaves preserved with silica gel at -80 ℃ were higher. [Conclusion]The study can provide the basis for studying the genetic diversity of Eomecon chinantha Hance..
2010年03期 v.1 9-11页 [查看摘要][在线阅读][下载 168K] [Objective] Comparison of the extraction methods of genomic DNA from the leaves of Tripterygium wilfordii Hook. f. [Method] With leaves of Tripterygium wilfordii Hook. f. as tested material, the genomic DNA was extracted using five different methods: CTAB method, modified CTAB method, SDS method, high salt and low pH method and ROSE method. The purity of the DNA was examined by agarose gel electrophoresis, OD260/OD280 and RAPD amplification, and the yield of the DNA was estimated by OD260. [Result] Modified CTAB method was the optimum one for DNA extraction. The ratio of OD260 and OD280 of the genomic DNA extracted by modified CTAB method was 1.869-1.903, and showed good performance in RAPD analysis. [Conclusion] The modified CTAB method is an optimum method for the extraction of genomic DNA from Tripterygium wilfordii Hook. f.
2010年03期 v.1 12-14+18页 [查看摘要][在线阅读][下载 250K] -
[Objective] The aim was to extract and isolate Bupleurum chinense DC. polysaccharide (BPP2), and study its molecular weight and monosaccharide composition. [Method] BPP2 was extracted from B. chinense with water, isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method, and TLC, HPLC were adopted to study its monosaccharide composition. [Result] The molecular weight of BPP2 was 67 836, which was composed of Rha, Xyl, Ara, Gal and Gle with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. [Conclusion] BPP2 is pure heterosaccharide which is composed of five kinds of monosaccharide, the molecular weight is 67 836.
2010年03期 v.1 15-18页 [查看摘要][在线阅读][下载 179K] [Objective]The biological characteristics of Limonium Bicolor were explored which provided a reference for its domestication and cultivation technique.[Method]Many years of field observation were documented, and then Limonium Bicolor were sowed in the greenhouse, cultivated with vegetable cultivation and management model.[Result]Observations and experiments showed Limonium Bicolor was a biennial plant, seldom flowered at the same year. Protective cultivation of Limonium Bicolor was easy with a survival rate of transplanting was over 92%,planting spacing over 30 cm was better.In the protected cultivation, flowering could be regulated by controlling density, nutrient supply and defloration. Making Limonium Bicolor flower once in a year, and in the next year of June is flower stage, multi-lateral branch flowering is promoted when picking up the flowers at the beginning of the flower stage.[Conclusion]Limonium Bicolor had higher ecological and economic benefits, the biomass of cultivated environment was four times than that under the natural environment, so the cultivation planting had a broad prospect.
2010年03期 v.1 19-20+23页 [查看摘要][在线阅读][下载 255K] -
[Objective] The research was to establish the method of determining the content of chlorogenic acid in compound Shilintong capsule. [Method] The high performance liquid chromatography was adopted: chromatographic column: ZORBAXSB-C18(5 μm, 4.6 mm×250 mm), moving phase: acetonitrile-0.2% phosphoric acid solution(8∶92 V/V), flowing speed: 1.2 ml/min, detected wavelength: 327 nm. [Result] Chlorogenic acid showed good linear relationship within the range of 0.04-0.08 μg/ml. The sample recovery rate was 101.7%, RSD was 1.88%(n=6). [Conclusion] Using high performance liquid chromatography to determine the content of chlorogenic acid in compound Shilintong capsule has many advantages, such as simple operation process, accuracy, good repetitiveness, high precision and strong specificity. Therefore, it could be used as the content determination standard of compound Shilintong capsule.
2010年03期 v.1 21-23页 [查看摘要][在线阅读][下载 141K] [Object] The aim was to determine the contents of Cu, Fe, Zn, Mn in different parts of Lippia nodiflora and explore the development and utilization value of L. nodiflora. [Method] By using mixed acid which contains nitric acid and perchloric acid to digest samples and by atomic absorption spectrometry, four kinds of trace elements Cu, Fe, Zn, Mn in root, stem, leaf of L. nodiflora were determined. [Result] The results showed that each part of L. nodiflora contained abundant Cu, Fe, Zn, Mn elements, among which Fe ranked first. [Conclusion] The contents of Fe, Mn, Zn in root, stem and leaf of L. nodiflora are higher, so it is appropriate for all herbs used for medicine, which is consistent with the formulation of traditional Chinese medicine encyclopaedia.
2010年03期 v.1 24-25页 [查看摘要][在线阅读][下载 76K] -
[Objective] The antibacterial activity of pigment from Kandelia candel was studied, in order to provide a reference for the evaluation and exploitation of the medicinal values of K.candel. [Method] Using filter paper method, taking the determination of inhibition zone as an inspection target, the inhibitory effects of K. candel on the growth of Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa were tested respectively. [Result] K. candel performed different degrees of inhibitory effects on three strains, the sequence was as follows: Bacillus subtilis was the most obvious, the next was Escherichia coli, and Pseudomonas aeruginosa was the weakest. [Conclusion] The K. candel pigment has obvious inhibitory effects. This test only selects three pathogenic strains, and more effective tests regarding more strains are needed, in order to evaluate the inhibitory effects of pigment comprehensively.
2010年03期 v.1 26-27+30页 [查看摘要][在线阅读][下载 94K] [Objective] This paper was to discuss the method for the determination of trace elements arsenic and mercury in Rhizoma paridis, which provided a reference for the evaluation system of toxic and side-effect of R. paridis. [Method] GFAAS was used, matrix modifier of nickel, palladium chloride dosage, acid medium, ashing temperature, atomization temperature and other test parameters were optimized, and the influence of matrix modifier on the content determination of arsenic and mercury in R. paridis was explored. [Result] The results showed that the matrix modifier Ni(NO3)2 and PdCl2 can not only improve the ashing temperature and atomization temperature of arsenic and mercury, but also improve the sensitivity and precision of determination, and the reproducibility was good. [Conclusion] This method is rapid and reliable. It is a simple and effective method for the determination of trace elements arsenic and mercury in R. paridis.
2010年03期 v.1 28-30页 [查看摘要][在线阅读][下载 140K] -
[Objective] The pollen viability of honeysuckle was determined at different storage conditions, which provided a reference for the production and research of honeysuckle. [Method] Taking the fresh pollen of honeysuckle as an experimental material, Ⅰ-KⅠ staining, TTC staining and culture medium germination methods were used to determine its fresh pollen ability. Effects of different storage conditions on the pollen germination were studied. [Result] The results showed that adding different concentrations of sucrose and boric acid to culture media had promoted effects on the pollen germination. When the concentrations were 150 and 30 mg/L, the germination rate reached the maximum value of 75.23% and 52.78%. Ⅰ-KⅠ staining was not suitable for the determination of pollen viability of honeysuckle, while TTC staining was suitable for it and the rate reached 80.46%. [Conclusion] This method is relatively accurate and simple. It is better for mid-low temperature storage(-80 ℃) than cold storage(4 ℃, -23 ℃), and it is not suitable for the normal condition.
2010年03期 v.1 31-33+36页 [查看摘要][在线阅读][下载 267K] [Objective] The aim was to discuss the inhibition of Cortex Moutan and Radix Paeoniae Rubra extract to bacteria and then provide further information to develop the two new medicine. [Method] Variant concentration of Cortex Moutan and Radix Paeoniae Rubra extract was studied to the inhibition of six food-borne microorganisms and pathogens and determination of the minimum inhibitory concentration (MIC) by employing filter paper diffusion method. [Result] The Cortex Moutan extract has stronger bacteriostasis activity to the S.typhimurium and E.coli with 0.09 and 0.11 mg/ml as their MIC, respectively, while relatively lower inhibition to S.aureus and S.faecalis; however, Radix Paeoniae Rubra extract has stronger bacteriostasis activity to the L.monocytogenes and S.typhimurium with 0.10 and 0.19 mg/ml as their MIC, respectively, but little inhibition to S.faecalis. [Conclusion] Both of the Cortex Moutan and Radix Paeoniae Rubra extract have some bacteriostasis activity to the six food-borne microorganisms and pathogens.
2010年03期 v.1 34-36页 [查看摘要][在线阅读][下载 92K] -
In order to optimize the extraction of volatile oil from cloves and provide a basis for modification of production processes, the extraction technology by ultrasonic method with the orthogonal experiments L9 (34) is conducted and the yield rate of volatile oil is used as an index. As a result, the extraction rate is up to 9% under this condition (i.e., ratio of solid-liquid, 1∶20; extraction time, 90 min; ethanol concentration, 75%; extraction temperature, 65 ℃). With GC-MS extraction technique, the components of clove volatile oil are isolated and 21 kinds are identified, accounting for 99.867% of total volatile oil, in which the contents of eugenol, isoeugenol and caryophyllene are 65.710%, 19.920% and 9.332%, respectively. Therefore, the main components of clove volatile oil by assisted-ultrasonic extraction technique are eugenol, isoeugenol and caryophyllene and this method to extract volatile oil from clove is much easier and more practical to operate.
2010年03期 v.1 37-40+45页 [查看摘要][在线阅读][下载 297K] The mechanism and research status of biotransformation were summarized.The feasibility of making use of this technology to improve the quality of traditional Chinese medicinal materials was discussed. Finally, the study pointed out that the technology has a wide application prospect in development and utilization of active ingredient in traditional Chinese medicine.
2010年03期 v.1 41-45页 [查看摘要][在线阅读][下载 141K] -
[Objective] This research was to establish a method for the determination of alpha-mangostin in Garcinia mangostana L. [Method] Reverse phase high-performance liquid chromatography(RP-HPLC) method with an ultra-violet detector(UVD) was performed on a 5 μm DiamonsilTM C18 column(250.0 mm×4.6 mm) at 25 ℃, the mobile phase composition was methanol-water(83∶17), the flow rate was at 1.0 ml/min, the detection wavelength was 317 nm. [Result] The method fulfilled all the standard requirements of precision, accuracy and linearity(r>0.999). The recovery was 98.9%, RSD was 1.87%. Alpha-mangostin contents of three batches samples of G. mangostana were 36.4, 37.3, 38.5 mg/g respectively, the average content was 37.4 mg/g. [Conclusion] RP-HPLC-UVD method is simple, accurate, high sensitivity, good reproducibility, and can be used for the determination of alpha-mangostin in G. mangostana.
2010年03期 v.1 46-47+51页 [查看摘要][在线阅读][下载 100K] Leonurus Heterophyllus is the whole plant of Leonurus Artemisia (a species of labiatae family), which is widely used in traditional Chinese medicine. Modern researches indicate that it is an important drug with wide applications in medicine and development prospect. Therefore, the progress in extraction method, purification method, chemical constituents, pharmacological function and cultivation technique of Leonurus Heterophyllus are reviewed in this paper so as to provide a reference for the further utilization in future.
2010年03期 v.1 48-51页 [查看摘要][在线阅读][下载 120K] -
[Object] The aim was to study the chemical components of essential oil in Buddleia officinalis Maxim and provide the scientific basis for further exploitation and utilization. [Method] The essential oil was extracted by steam distillation. The chemical components of the essential oil were analyzed by GC-MS. The relative contents of these components were calculated using square peaks to normalization. [Result] 46 peaks were separated and 27 components were identified, which were composed of about 72.41% of the total essential oil. [Conclusion] The main components are palmiticacid(14.07%), 6, 10, 14-trimethyl-2-pentadecanone(12.92%), heneicosane(6.15%), and heptacosane(3.28%).
2010年03期 v.1 52-53页 [查看摘要][在线阅读][下载 107K] In order to establish the method for mensurating total flavonoids content in Camellia chrysantha (Hu) Tuyama, UV spectrophotometry method is employed. As a result, in the range of 0.008 64-0.069 12 mg/ml, the linear relationship is good(r=0.999 9), the average recovery rate is 100.4% and RSD is 0.018 0%. Therefore, the method is simple and reliable and thus can be used to control the quality of C. chrysantha.
2010年03期 v.1 54-55页 [查看摘要][在线阅读][下载 68K] -
The application of Rheum purification methods such as ultrasonic extraction, microwave extraction, supercritical CO2 extraction, macroporous adsorption resin etc. were summarized.
2010年03期 v.1 56-60页 [查看摘要][在线阅读][下载 130K] [Objective] This paper was to study the chemical components of volatile oil from Polygala tenuifolia Willd. which could provide a reference for evaluating its medicinal value. [Method] The volatile oil from P. tenuifolia was extracted by simultaneous distillation and extraction(SED), then its chemical compositions were analyzed by GC-MS. [Result] The results showed that 16 compounds were separated from the volatile oil of P. tenuifolia, and 14 compounds of the formula, molecular weight, and relative content were identified. [Conclusion] Oleic acid is the major chemical constituents in the volatile oil, which accounts for 52.81%, the next are the palmitic acid and steraic acid, which account for 28.26% and 12.78% respectively.
2010年03期 v.1 61-62页 [查看摘要][在线阅读][下载 98K] -
<正>TROPAG & RURAL,produced by the Information,Library and Documentation department of the Royal Tropical Institute,The Netherlands,are two databases containing full bibliographic references with abstracts to the worldwide literature on agriculture and economic and social development.
2010年03期 v.1 62页 [查看摘要][在线阅读][下载 81K] 下载本期数据