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[Objective] To obtain the optimum medium formula for proliferation of D. candidum protocorm. [Method] The optimum concentration ratio of 6-BA, NAA and KT was screened by orthogonal test, and effect of each factor on the proliferation of D. candidum protocorm was investigated. [Result] The optimum medium formula was: 1/2MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L KT. [Conclusion] The study provides basis for the industrialized production of protocorm and breeding high quality D. candidum.
2011年02期 v.2 1-2页 [查看摘要][在线阅读][下载 106K] [Objective] To select the optimum hormones ratio and concentration for the scale cutting propagation of Lycoris plants, so as to provide basis for the production of Lycoris plants in winter. [Method] 4 concentrations of IBA and NAA were combined randomly into 16 formulas. [Result] Different concentrations of IBA and NAA have a certain effect on the multiplication coefficient, bulb mean weight and average rooting number, and the concentration of NAA-IBA mixed solution was proved to be the best in the range of 25-50 mg/L; under this condition, the multiplication coefficient of Lycoris plants was higher with lower bulb mean weight. [Conclusion] It is feasible to carry out scale cutting propagation of Lycoris radiate and Lycoris sprengeri in winte.
2011年02期 v.2 3-5页 [查看摘要][在线阅读][下载 98K] -
[Objective] This study was to investigate the accumulation and absorption characteristics of Solanum torvum Swartz. to heavy metals from soil, so as to guarantee the safe use of traditional Chinese medicine. [Method] Using S. torvum sampled from its main distribution areas as experimental materials, the contents of eight heavy metals of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn in the roots, stems and the habitat soil these plants grew were determined to analyze the distribution law of heavy metals in different parts of S. torvum and the soil, as well as the accumulation and transport capacity of S. torvum plants sampled from different producing areas to heavy metals. [Result] S. torvum and corresponding habitat soil collected from different sampling plots differed significantly in the contents of heavy metals. Contents of Cu, Pb and As in roots of S. torvum exceeded the national standard, and the absorption of S. torvum to heavy metals was interactional. The contents of accumulated Cu and Ni in roots of S. torvum ranked the first and second, respectively, whereas all the heavy metals did not accumulate remarkably in stems and displayed a lower transport coefficient. [Conclusion] S. torvum roots accumulated most of the heavy metals and its stems could be used as the main medicinal parts.
2011年02期 v.2 6-9页 [查看摘要][在线阅读][下载 98K] [Objective] The resource of the weeping forsythia would comprehensively be developed. [Method] The mutant of weeping forsythia-Goldvein was introduced from Canada and its genetic stability in seedling multiplication, regional trial and extensive planting was observed. [Result] The special character of the variety could stably be kept from the parent to next generation and the genetic stability and coincidence was also showed in the new variety, which was with good developing prospect in actual production. [Conclusion] The theoretic basis of the utilization of weeping forsythia was provided through the introduction of its planting, multiplication and cultivation management.
2011年02期 v.2 10-12页 [查看摘要][在线阅读][下载 91K] -
[Objective] To study the effects of salt stress on the contents of proline in the leaves of different varieties of Lonicera japonica Thunb.. [Method] The seedlings of Lonicera japonica Thunb. were treated with different concentrations of NaCl solution (0,100,200,400 and 600 mmol/L), and the proline content can be used as indicator for evaluating the salt tolerance of Lonicera japonica Thunb..[Result] After short treatment, the content of proline in the leaves of treelike Lonicera japonica Thunb. increased dramatically with the concentration of NaCl increased; although there were no significant difference among the vinelike Lonicera japonica Thunb. groups, the content of proline in the leaves of vinelike Lonicera japonica Thunb. increased dramatically with the concentration of NaCl increased as time went by. The contents of proline in treelike and vinelike Lonicera japonica Thunb. achieved the highest level when the concentration of NaCl was 400 mmol/L. [Conclusion] The proline in the leaves of treelike Lonicera japonica Thunb.was more sensitive to salt stress and showed stronger osmotic regulation ability.
2011年02期 v.2 13-14+17页 [查看摘要][在线阅读][下载 123K] [Objective] To carry out preliminary research on main chemical components of Lethariella zahlbruckneria, so as to lay a foundation for further isolation and structure identification of active components. [Method] A pre-test for chemical components of Lethariella zahlbruckneria was performed by combining test tube method with thin-layer chromatography. [Results] Phenols, volatile oil, organic acid, polysaccharide and glycoside were main chemical components of Lethariella zahlbruckneria. Besides, it may contain cardiac glycoside, sterides and hemiterpene. [Conclusion] This research can provide a basis for taxonomic studies of lichen that belongs to Parmeliaceae family, Lethariella genus.
2011年02期 v.2 15-17页 [查看摘要][在线阅读][下载 87K] -
[Objective] The research aimed to choose appropriate conditions for determining trace antimony in RADIX ACHYRANTHIS BIDENTATAE from different producing areas by hydride generation-atomic fluorescence spectrometry, and to measure the content of trace antimony. [Method] Effects of factors such as acidity, concentration of potassium borohydride and pre-reducing agent on the analytical sensitivity were investigated. [Result] Under the optimum conditions, parameters of antimony were: detection limit of 0.05 μg/L, linear range of 0.05-100 μg/L, relative standard deviation value (RSD) of 1.36%, recovery of 93.1%-106.5% and antimony content of 0.059-0.772 μg/g. [Conclusion] The method was simple and easy to be operated, rapid, high sensitive and less interference.
2011年02期 v.2 18-20页 [查看摘要][在线阅读][下载 90K] [Objective] To research the inhibitory effects of polysaccharides from Dendrobium officinale and Dendrobium hookerianum on Staphyloccocus aureus, Escherichia coli and Bacillus subtilis in vitro.[Method] The antibacterial activity of two Dendrobium polysaccharides on the above bacteria were explored by filter paper disk method.[Results] Polysaccharide from Dendrobium officinale showed the most remarkable antibacterial effect on Escherichia coli, i.e., the minimal inhibitory concentration(MIC) was 0.5%, and the diameter of inhibition zone was 15.8 mm; polysaccharide from Dendrobium hookerianum showed the most remarkable antibacterial effect on Bacillus subtilis, i.e., MIC was 0.5%, and the diameter of inhibition zone was 12.8 mm. [Conclusion] Polysaccharide from Dendrobium officinale had antibacterial effect on Escherichia coli and Bacillus subtilis, while that from Dendrobium hookerianum showed antibacterial effect on all tested bacteria.
2011年02期 v.2 21-22页 [查看摘要][在线阅读][下载 72K] -
[Objective] To study antibacterial activities of crude extracts from the whole herb of Orostachys cartilaginous A.Bor in vitro. [Method] liquid-liquid extraction technique was applied to isolate active ingredients from 0.75 acetone (volume fraction) extracts for preparing chloroform, ethyl acetate, normal butanol and water phases. The antibacterial activities of extraction phases against 4 tested strains in vitro were investigated by combining filter paper method with double dilution method. [Result] Extraction phases from 0.75 acetone extracts had antibacterial activities against Staphyloccocus aureus Rosenbach, Bacillus subtilis (Ehrenberg) Cohn, Escherichia coli and Proteus vulgaris in varying degrees. Chloroform and ethyl acetate extraction phases were provided with strong antibacterial activities against Staphylococcus aureus with minimal inhibitory concentration of 1.25 mg/ml. [Conclusion] Active ingredients of Orostachys cartilaginous A.Bor plant were mainly existed in chloroform and ethyl acetate phases, which could provide a scientific basis for developing antibacterial agents from Orostachys cartilaginous A.Bor.
2011年02期 v.2 23-25页 [查看摘要][在线阅读][下载 97K] [Objective] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method] The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPLC. The chromatographic conditions were as follows: column of Agilent Zorbax XDB-C18 (4.6 mm×150 mm, 5 μm), mobile phase of acetonitrile (A)-0.2% phosphoric acid solution (B) (gradient elution: 0-20 min, 0-20% A), flow rate of 1.0 ml/min, column temperature of 30 ℃, sampling volume of 10 μl and detection wavelength of 275 nm. [Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg/ml (r=0.999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. with 5.59%, and the lowest in Rodgersia aesculifolia Batalin with 3.45% among 4 species and 3 varieties of genus Rodgersia. [Conclusion] The bergenin content in the genus Rodgersia was various in different species, and there were obvious differences among different populations from the same species in content.
2011年02期 v.2 26-29+33页 [查看摘要][在线阅读][下载 159K] -
[Objective] To establish a method for determining the contents of hyperoside in ethanolic extract of Abelmoschus manihot (L.) Medic, and study its growth inhibitory effect on Hela cells. [Method] Using 80% ethanol as solvent for extracting total flavonoids, the contents of hyperoside in different parts of Abelmoschus manihot (L.) Medic were determined by HPLC. The HPLC conditions were: chromatographic column of Yilite C18 column (4.6 mm ×250 mm, 5 μm), mobile phase of methanol and 0.05% phosphoric acid solution (V/V, 60∶40), flow rate of 1.0 ml/min, column temperature of 25 ℃, detection wavelength of 360 nm and sampling volume of 10 μl. Growth inhibitory effect of ethanolic extract on Hela cells was assessed by MTT assay. [Results] The hyperoside presented a good linear relationship with peak area in the range of 0.04 to 0.4 mg/ml with correlation coefficient at 0.999 8. The content of hyperoside decreased in the following order, flowers, leaves, stems, roots and seeds. The average content in flowers was the highest that up to 4.002%. The average recovery and RSD value were 99.23% and 1.08% (n=6), respectively. Flavonoids extract of Abelmoschus manihot (L.) Medic could effectively suppress the growth of Hela cells, IC50 of which was 228 μg/ml. [Conclusion] The method was accurate, simple and reproducible that could be used for determining the hyperoside in Abelmoschus manihot (L.) Medic. The flavonoids extract was provided with strong growth inhibitory effect on Hela cells.
2011年02期 v.2 30-33页 [查看摘要][在线阅读][下载 163K] <正>Naresuan University Library is situated in the main campus ( Nong-Or) Established in 1993. The present library building has 3 storeys with approximately 4 920 square meters. In 1994 ,the Health Sciences Library was established as a department of the
2011年02期 v.2 33页 [查看摘要][在线阅读][下载 64K] -
[Objective] To determine the contents of trace elements in RADIX PUERARIAE THOMSONII. [Method] Flame atomic absorption spectrometry (FAAS) method was adopted to determine the contents of 4 trace elements including Mg, Fe, Mn and Zn in 7 kinds of RADIX PUERARIAE THOMSONII. [Results] RADIX PUERARIAE THOMSONII was rich in Mg, Fe, Mn and Zn; contents of trace elements were various not only in different producing areas and species, but also in different species from the same producing area. [Conclusion] This research could provide reference for further exploitation and utilization of RADIX PUERARIAE THOMSONII resource.
2011年02期 v.2 34-35+52页 [查看摘要][在线阅读][下载 99K] [Objective] To determine the types and contents of amino acids in the flowers of Fritilaria thunbergii Miq., so as to provide scientific basis for its further development.[Method] Natural dried, lyophilized and fluidized bed treated flowers of Fritillaria thunbergii Miq. were as test materials, and the content of amino acids in which were determined by ninhydrin column derivatization method. [Result] There were 17 types of amino acids in the flower of Fritillaria thunbergii Miq., and the total contents of amino acids in different treated Fritillaria thunbergii Miq.flowers achieved 200 g/kg, among which the content of glutamic acid was the highest, and the essential amino acids accounted for more than 30% in total.[ Conclusion] The flowers of Fritillaria thunbergii Miq.were endowed with high medical and healthy value, which deserve further research.
2011年02期 v.2 36-37+56页 [查看摘要][在线阅读][下载 94K] -
[Objective] To investigate the effects of different digestion procedures on microelements contents (Fe, Mn, Zn, Cu) in Taraxacum mongolicum Hand.-Mazz.. [Method] The Taraxacum mongolicum Hand.-Mazz. samples were treated by dry ashing, wet ashing and microwave digestion methods respectively, and the contents of Fe, Mn, Zn, Cu were determined by flame atomic absorption spectrometer(FAAS). [Result] The contents of Cu, Zn, Fe, Mn were the largest in microwave treated sample, which achieved 35.01, 103.11, 210.86, 21.13 μg/g respectively. [Conclusion] Microwave digestion method was proved to be suitable for the pretreatment of plant samples.
2011年02期 v.2 38-39+56页 [查看摘要][在线阅读][下载 84K] [Objective] To discuss the effects of arsenic stress on the activities of antioxidant enzymes in medicinal plant Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao, so as to provide a theoretical basis for revealing the arsenic toxicity mechanism on plants. [Method] Using Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as tested materials, potassium permanganate titration, guaiacol method, pyrogallol autooxidation methods and thiobarbituric acid method were respectively adopted to study effects of arsenic stress on catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) activities and malondialdehyde (MDA) concentrations. [Results] With the increase in concentration of arsenic, the activities of CAT, PCD and SOD in Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.)Hsiao could be maintained, especially POD. From the view point of dynamic time, CAT activity increased firstly then declined, POD activity increased and SOD activity changed little along with the increasing treatment time. Besides, MDA content increased firstly and then decreased that reached the maximum on 8th day. [Conclusions] The study could provide theoretical basis for control of heavy metals in traditional Chinese medicine and identification of pharmacological activities.
2011年02期 v.2 40-42+59页 [查看摘要][在线阅读][下载 245K] -
[Objective] To sub-clone and express glycosidase gene of Dioscorea nipponica. [Method]The glycosidase gene of Dioscorea nipponica was sub-cloned into Pichia pastoris GS115 expression vector pPIC9K, and the constructed expression vector was introduced into Pichia pastoris GS115 by electroporation method. [Result]After have been cultivated for 144 hours in 0.5% methanol, the glycosidase gene in recombinant was proved to be active, and the relative molecular weight of which was 58 kDu through SDS-PAGE analysis. [Conclusion]Glycosidase gene of Dioscorea nipponica was expressed successfully.
2011年02期 v.2 43-45+47页 [查看摘要][在线阅读][下载 402K] [Objective] To identify the genuine and false FLOS CHRYSANTHEMI INDICI by HPLC, so as to provide basis for the standardization of traditional Chinese medicine market. [Method] By determining the content of buddleoside and chlorogenic acid, the genuine and false FLOS CHRYSANTHEMI INDICI were identified. HPLC conditions were as follows: mobile phase of methanol-water- glacial acetic acid (V∶ V∶ V=26∶31∶1), detection wavelength of 334 nm, flow rate of 1 ml/min, column temperature of 35 ℃ and sampling amount of 10 μl. [Result] Contents of buddleoside and chlorogenic acid in genuine FLOS CHRYSANTHEMI INDICI were 8.365 and 0.228 mg/g respectively, while which were not detected in false FLOS CHRYSANTHEMI INDICI. [Conclusion] The method established in this study was convenient and feasible, which could be employed to distinguish genuine and false FLOS CHRYSANTHEMI INDICI.
2011年02期 v.2 46-47页 [查看摘要][在线阅读][下载 162K] -
[Objective] To distinguish Gentiana straminea Maxim. from wild and cultivated plants by comparing chloroplast DNA psbA-trnH sequences, so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH sequences of Gentiana straminea Maxim. were amplified by polymerase chain reaction (PCR), and then sequenced by direct PCR sequencing method for homologous analysis. [Result] The lengths of cpDNA psbA-trnH from wild and cultivated plants were 316 and 317 bp respectively, and there were 4 variable sites.[Conclusion] The nucleotide differences of psbA-trnH regions could be used for distinguishing Gentiana straminea Maxim. from wild and cultivated plants.
2011年02期 v.2 48-49+62页 [查看摘要][在线阅读][下载 86K] [Objective] To investigate the effect of different methods on the extraction of rutin and quercetin in Rosa Laevigata Michx., so as to provide scientific basis for its reasonable development. [Method] Rutin and quercetin in Rosa Laevigata Michx. were extracted by hot water soaking, alcohol reflux and alcohol-ultrasonic assisted methods, and the contents of which were determined by HPLC, methodology investigation was carried out as well. [Result] Rutin and quercetin were separated well by HPLC, which had good relationships with peak areas in the range of 0.03-15 μg/ml (r=0.999 0) and 0.04-20 μg/ml(r=0.999 4) respectively, and the limits of detection (LOD) were 12.6 and 15.2 ng/ml. The extraction effect of the above three methods was in the order of alcohol-ultrasonic assisted method>alcohol reflux>hot water soaking, and the yield rates of rutin were 0.62, 1.04, 1.52 μg/ml respectively; the yield rates of quercetin were 0.78, 1.18 and 2.24 μg/ml.[Conclusion] Alcohol-ultrasonic assisted method was proved to be the best way to extract rutin and quercetin from Rosa Laevigata Michx.
2011年02期 v.2 50-52页 [查看摘要][在线阅读][下载 146K] -
[Objective] To optimize the extraction conditions of polysaccharides from the root tuber of Ipomoea batatas (Linn.) Lam.. [Method] Polysaccharides were extracted from the root tuber of Ipomoea batatas (Linn.) Lam. by ultrasonic extraction method, whose extraction conditions were optimized by combining orthogonal test design with single factor experiment. [Result] The influencing order of each factor on yield of polysaccharides was determined to be: ultrasonic frequency>solid-liquid ratio>extraction temperature>extraction time. The optimum conditions for extracting polysaccharides from the root tuber of Ipomoea batatas (Linn.) Lam. were as follows: solid-liquid ratio of 1∶15, extraction time of 40 min, extraction temperature of 60 ℃ and ultrasonic frequency of 80 kHz. Under the optimum extracting conditions, the extraction rate of polysaccharides reached as high as 3.63%. [Conclusion] The extraction process of polysaccharides from the root tuber of Ipomoea batatas (Linn.) Lam. by ultrasonic technology was high in yield and short in extraction time. If carried out at low temperature, the extraction was less in extracting solvent and less in cost.
2011年02期 v.2 53-56页 [查看摘要][在线阅读][下载 228K] <正>TROPAG & RURAL,produced by the Information,Library and Documentation department of the Royal Tropical Institute, The Netherlands,are two databases containing full bibliographic references with abstracts to the worldwide literature on agriculture
2011年02期 v.2 56页 [查看摘要][在线阅读][下载 60K] -
[Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content, so as to provide scientific evidence for the development, application and quality control of ursolic acid. [Method] The extraction conditions of ursolic acid were optimized by orthogonal test, and content of ursolic acid was determined by HPLC. [Result] The optimal extraction conditions were as follows: extraction duration of 30 min, extraction solvent of absolute ethanol, ethanol dosage of 25.0 ml. Under the optimum conditions, the content of ursolic acid was 0.324%. Ursolic acid concentration had good linear relationship with peak area within the range of 0.650-3.250 μg/ml (r=0.999 1), and the mean recovery rate of ursolic acid was 99.20% with RSD of 0.30%. [Conclusion] The optimum extraction conditions were stable and efficient, and HPLC method was simple, sensitive and accurate. This study provided scientific evidence for the utilization and quality control of ursolic acid.
2011年02期 v.2 57-59页 [查看摘要][在线阅读][下载 129K] <正>CAS is a team of scientists,creating and delivering the most complete and effective digital information environment for scien- tific research and discovery. CAS provides pathways to published research in the world's journal and patent literature-virtually eve-
2011年02期 v.2 59页 [查看摘要][在线阅读][下载 57K] -
[Objective] To obtain high-quality beverage prepared by flower of Syringa reticulata Blume. [Method] The Syringa reticulata Blume flower beverage was prepared by fermentation method. Using sensory score of the beverage as indices, the preparation parameters were optimized by orthogonal test based on single factor test. [Result] The optimal preparation parameters for Syringa reticulata Blume flower drink were determined to be: added amount of crude juice of 20.0%, 8.00 g of white granulated sugar and 0.75 g of citric acid. The beverage prepared under the optimum parameters was endowed with good sensory characteristics like good mobility, even luster and fine odor etc.. [Conclusion] The high-quality Syringa reticulata Blume flower beverage was obtained in this study, which provided basis for the development of Syringa reticulata Blume flower.
2011年02期 v.2 60-62页 [查看摘要][在线阅读][下载 87K] <正>Centre for Agriculture and Bioscience International( CABI) is a not-for-profit international Agricultural information Institute with headquarters in Britain. It aims to improve people's lives by providing information and applying scientific expertise to solve prob-
2011年02期 v.2 62页 [查看摘要][在线阅读][下载 56K] -
[Objective] To compare the chemical constituents of artificial induced Dalbergia Odorifera heartwood with those of natural Dalbergia Odorifera heartwood. [Method] The chemical ingredients of Dalbergia Odorifera were refluxing extracted by absolute ether and determined by TLC. [Result] The chemical ingredients between induced and natural Dalbergia Odorifera heartwood were the same on the whole. [Conclusion] The formation of Dalbergia Odorifera can be accelerated by artificial method, which offers scientific evidence for its further utilization.
2011年02期 v.2 63-64页 [查看摘要][在线阅读][下载 132K] [Objective] To optimize the extraction process of total flavonoids from RADIX RHIZOMA GLYCYRRHIZAE. [Method] Total flavonoids were extracted from RADIX RHIZOMA GLYCYRRHIZAE with ethanol by ultrasonic-assisted method, whose extraction process was optimized by orthogonal test design. [Result] The optimum extraction process was as follows: ethanol concentration of 75%, solid-liquid ratio of 1∶25, ultrasonic power of 350 W and ultrasonic duration of 25 min. Under the above conditions, the extraction rate of total flavonoids reached as high as 1.943%. The influencing order of each factor on extraction rate of polysaccharides was determined to be: ethanol concentration>solid-liquid ratio>ultrasonic power>ultrasonic duration. [Conclusion] The study could provide a scientific basis for the further exploitation and utilization of RADIX RHIZOMA GLYCYRRHIZAE.
2011年02期 v.2 65-67+71页 [查看摘要][在线阅读][下载 232K] -
[Objective] To optimize the extracting conditions of antioxidant components from Arctium lappa L., and determine their antioxidant activity. [Method] The extraction conditions were optimized by orthogonal test. The scavenging effects of Arctium lappa L. extracts on superoxide anion radical and hydroxyl radical were determined by conventional methods. [Result] The optimal extracting conditions were as follows: solid-liquid ratio of 1∶20, ethanol concentration of 100%, extraction duration of 1.5 h and extraction temperature of 70 ℃. The scavenging effects of Arctium lappa L. extracts on superoxide anion radical and hydroxyl radical showed upward trend with the increase of concentration, and the antioxidant activity of ethanol extracts was stronger than that of water extracts. Chlorogenic acid was the main component of Arctium lappa L. extracts. [Conclusion] This study provides scientific basis for the application of Arctium lappa L. extracts in food and pharmaceutical industries.
2011年02期 v.2 68-71页 [查看摘要][在线阅读][下载 333K] 下载本期数据