Review

  • Advance in Pathogenesis of Gout and Related Gene Polymorphism

    Lamei ZHOU;Wen XU;Chunqing LI;

    Gout is an independent risk factor for hypertension, diabetes mellitus, hyperlipidemia, coronary heart disease and cerebral infarction. At present, the studies on the mechanism of gout at home and abroad have mainly focused on immune inflammation, gene polymorphism and related studies. Uric acid deposition or crystal precipitation activates phagocytes, fibroblasts and mast cells in synovium, produces IL-1β, TNF and chemokine IL-8(CXCL8), thereby activating neutrophils, urate crystal polyanion surface can be coated with immunoglobulins and other serum proteins as substrates for complement activation, complement substitution pathways and classical complement pathways to activate complements. Uric acid crystals activate inflammation-related signal transduction pathways including the activation of inflammation-related signal transduction pathway by uric acid crystals and TLRS/MyD88 signal transduction pathways. Gene polymorphism is related to inflammation and signaling pathway, EGF gene is closely related to gout inflammation, which may be involved in the regulation of gout inflammation, among which NLRP3 inflammatory signaling pathway and gene polymorphism have been deeply studied in the pathogenesis of gout, which is the main therapeutic target of anti-inflammatory and uric acid lowering.

    2019年06期 v.10 1-3+5页 [查看摘要][在线阅读][下载 150K]
  • Pharmacological Activity and Application of Roots, Stems and Leaves of Scutellaria baicalensis Georgi

    Xinyang LIU;Yuanyuan YE;Qianqian LIU;Shengkai DING;Yazhen SHANG;

    The root of Scutellaria baicalensis Georgi is traditionally used as medicine, and it has been confirmed that S. baicalensis Georgi has flavonoid chemical constituents, pharmacological activity and cosmetic efficacy. With the extensive application of S. baicalensis Georgi roots, the resource of S. baicalensis Georgi has been increasingly short. The above-ground part of stems and leaves of S. baicalensis Georgi has also been gradually recognized and developed. Studies have found that the chemical constituents from stems and leaves of S. baicalensis Georgi are also a group of flavonoids with a lot of pharmacological activity and have a great application value. Based on this, the present review will be reported on the chemical constituents and application of the roots, stems and leaves of S. baicalensis Georgi.

    2019年06期 v.10 4-5页 [查看摘要][在线阅读][下载 120K]
  • Systematic Evaluation and Meta-analysis of Modified Baitouweng Decoction in the Treatment of Ulcerative Colitis

    Bing WEI;Yi ZHANG;Ningning ZHAO;Shiying TANG;

    [Objectives] To evaluate the clinical efficacy and safety of Modified Baitouweng Decoction in the treatment of ulcerative colitis(UC). [Methods] A randomized controlled trial of Modified Baitouweng Decoction in the treatment of ulcerative colitis was conducted, and the biased risk was evaluated in the included literature using a standardized method. A meta-analysis of the total effective rate and recurrence rate of the included literature was carried out. Sensitivity and safety analysis was carried out on the included literature. [Results] A total of 19 articles were included, involving a total of 1 613 patients. The results of meta-analysis showed that the treatment of Modified Baitouweng Decoction alone or in combination with the western medicine is better than the treatment with the western medicine alone. [Conclusions] Modified Baitouweng Decoction is safe and effective in the treatment of ulcerative colitis. However, since the 19 articles included in this study are not high in quality, they have certain influence on the objectivity of the results.

    2019年06期 v.10 6-9+14页 [查看摘要][在线阅读][下载 1012K]
  • Nomenclature Origin and Development of Bi Syndrome Prescriptions

    Lamei ZHOU;Yuanyuan DING;Donglin HAO;

    More than 40 medical history and classical medical literature on the nomenclature of Bi syndrome were systematically arranged, induced and analyzed, the prescriptions named after the medicine include RHIZOMA ET RADIX NOTOPTERYGII, os tigris, RADIX ASTRAGALI, RADIX SAPOSHNIKOVIAE, Prepared common monkshood daughter root, doubleteeth pubescent angelica root,RADIX ANGELICAE SINENSIS, frankincense, RADIX STEPHANIAE TETRANDRAE, RADIX ACHYRANTHIS BIDENTATAE, myrrh, RADIX ACONITI、RADIX DIPSACI, RHIZOMA GASTRODIAE, ginseng root, HERBA DENDROBII, CORNU SAIGAE TATARICAE, RADIX MORINDAE OFFICINALIS, PORIA, SEMEN COICIS, poison yam, and so on. It basically reflected the ancient and modern treatment of Bi syndrome in traditional Chinese medicine named after the formula.

    2019年06期 v.10 10-14页 [查看摘要][在线阅读][下载 158K]

Chemical Composition of Natural Medicine

  • Extraction Process and Content Determination of Caffeic Acid in Zhuang Medicine Cryptolepis buchananii

    Chenyan LIANG;Lu ZHANG;Yunli TANG;Liuyan HE;Ganneng HUANG;

    [Objectives] To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii. [Methods] The content of caffeic acid in C. buchananii leaves was determined by high performance liquid chromatography(HPLC). [Results] The results of HPLC determination showed that the RSD values were less than 3%, and the content of caffeic acid in 10 batches of C. buchananii leaves was in the range of 0.096 5%-0.477 2%. [Conclusions] This method is accurate and reliable, has good linear relationship and high precision, and can be used for quality evaluation of C. buchananii.

    2019年06期 v.10 15-19页 [查看摘要][在线阅读][下载 1507K]
  • Stability of Sanguinarine and Chelerythrine in Macleaya cordata (Willd.) R. Br.

    Chunmei LI;Xu CHEN;Wenlan LI;Bingmei LIU;Li WANG;Yubin JI;

    [Objectives] To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.) R. Br. [Methods] The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC. Besides, the effects of water quality, light source, oxidant, temperature, and pH on stability were investigated. [Results] The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good; the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable, but they are unstable in tap water whether they are protected from light or not; oxidants have a great influence on the stability of sanguinarine and chelerythrine; sanguinarine and chelerythrine are stable at room temperature lower than 54 ℃; sanguinarine is stable in pH 2.5-7.0, while chelerythrine is stable in pH 2.5-8.0. [Conclusions] The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.

    2019年06期 v.10 20-23页 [查看摘要][在线阅读][下载 967K]
  • Chemical Composition of Rubus pirifolius Smith

    Liuping WANG;Danfeng GUO;Shuhui LI;Zhiqi HUANG;Dongping TU;Jianhua WEI;

    [Objectives] This paper aimed to study the chemical composition of Rubus pirifolius Smith [Methods] A preliminary experiment was carried out using the qualitative reactions of various chemical constituents, in order to roughly detect the types of chemical constituents that may be contained in R. pirifolius Smith The ethanol extract of R. pirifolius Smith were separated and purified using silica gel column chromatography, silica gel thin-layer chromatography and recrystallization, and the chemicals obtained were identified using mass spectrometry, nuclear magnetic resonance and literature reference. [Results] The main constituents of R. pirifolius Smith are saponins, tannins, flavonoids, phytosterols, triterpenes and anthraquinones. A total of five compounds were isolated from the ethanol extract of R. pirifolius Smith, and they were identified as β-sitosterol(1), arjunolic acid(2), stigmasterol-3-O-β-D-glucopyranoside(3), euscaphic acid(4) and 3, 3′, 4-trimethyle llegic acid(5). [Conclusions] Compounds 1, 2, 3, 4 and 5 were isolated from R. pirifolius Smith for the first time.

    2019年06期 v.10 24-26+34页 [查看摘要][在线阅读][下载 148K]
  • Simultaneous Determination of Four Phenolic Acids in Radix Salviae Miltiorrhizae Formula Granules by QAMS

    Hongyan WEI;Qiongxi YU;Jinguang LU;Zhihong JIANG;Jingzheng SONG;Jian ZHOU;

    [Objectives] This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker) method for simultaneous determination of four phenolic acids, salvianolic acid B, tanshinol, lithospermic acid and rosmarinic acid in Radix Salviae Miltiorrhizae by HPLC and conduct methodological investigation. [Methods] Salvianolic acid B, with moderate retention time, good resolution, great response value and easy availability was selected as the internal standard, and the relative retention values and relative correction factors between tanshinol, rosmarinic acid, lithospermic acid and salvianolic acid B were established. The results of QAMS were compared with those of external standard method to verify the accuracy and applicability of QAMS. [Results] There were no significant differences between the calculated values of QAMS and the measured values of external standard method. The relative deviation was less than 3%, and the relative correction factors obtained from the experiment were credible. [Conclusions] QAMS can be used to determine the contents of phenolic acids in Radix Salviae Miltiorrhizae formula granules.

    2019年06期 v.10 27-29+38页 [查看摘要][在线阅读][下载 256K]
  • Determination of Naringin Content in Peel of Guangxi Citrus maxima (Burm.) Merr by HPLC

    Chenyan LIANG;Lu ZHANG;Cuiliu PAN;Yunli TANG;Rongling MA;

    [Objectives] To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.) Merr. [Methods] The high performance liquid chromatography(HPLC) method was applied. The chromatographic column was Agilent HC-C_(18)(4.6 mm×250 mm, 5 μm); the mobile phase was acetonitrile-0.1% phosphoric acid(18∶82); the flow rate was 1.0 mL/min; the column temperature was 25 ℃; the detection wavelength was 283 nm. [Results] Naringin showed a good linear relationship in the range of 0.164-3.27 μg, r=0.999 9. The average recovery rate was 98.66%, and RSD=1.80%(n=6). [Conclusions] This method is simple, feasible, reproducible, and accurate, so it can be used for the determination of naringin content in the peel of Guangxi C. maxima.

    2019年06期 v.10 30-31+51页 [查看摘要][在线阅读][下载 248K]

Pharmacological and Toxicological Analyses

  • Acute Toxicity Test of Abrus cantoniensis Hance Extract

    Xuankai HU;Haichun LONG;Qiao WEI;Yuxia WEI;Jinli HUANG;Tingshu LI;Zhidong LEI;Suoyi HUANG;

    [Objectives] To investigate the acute toxicity of petroleum ether extract, n-butanol extract, ethyl acetate extract and water extract to mice. [Methods] The medium lethal dose(LD_(50)) in mice could not be determined, so the maximum dose method was used for experiment. Mice were not fed but allowed to drink water for 16 h before the experiment. There were 74 SPF Kunming Mice, half male and half female, and it was divided into extract group and blank control group. The toxicity and death were recorded after intragastric administration and regular close observation. If any mice died, the dead mice were dissected. The changes of heart, liver, spleen, lung, kidney, brain, stomach, small intestine and other organs were observed with the naked eye. At the end of the 14 d observation period, the surviving mice in each group were killed and dissected. The gross pathological changes of the main organs were observed with the naked eye according to the same method mentioned above. [Results] There were no obvious toxic symptoms and no death in the mice. [Conclusions] The maximum doses of petroleum ether extract, n-butanol extract, ethyl acetate extract and water extract in mice were 1.61, 1.71, 1.65 and 1.79 g/L, respectively. Thus it was found that the quarterly tests of petroleum ether extract, n-butanol extract, ethyl acetate extract and water extract suggested that the medicine was safe.

    2019年06期 v.10 32-34页 [查看摘要][在线阅读][下载 128K]
  • Effects of Manshenkangning Prescription on Adenine-induced Renal Interstitial Fibrosis in Rats

    Linghui NIE;Aishan SU;Weifeng FENG;Long YI;Shiping ZHU;

    [Objectives] To study the protective effects of Manshenkangning Prescription on adenine-induced renal interstitial fibrosis in rats, and explore the possible mechanism. [Methods] Sixty Wistar male rats were divided into normal group, model group, control group(administered with 10 mg/(kg·d) losartan) and high, medium and low dose experimental groups(30, 15, 7.5 mg/(kg·d) Manshenkangning). The rat models of renal interstitial fibrosis were induced by intragastric administration of adenine(250 mg/(kg·d)). After 2 h, the above drugs were administered intragastrically for 21 consecutive days and the administration time was 30 consecutive days. Serum creatinine(SCr), blood urea nitrogen(BUN), 24 h urinary protein(24 h MTP) and glomerular filtration rate(eGFR) were measured by biochemical method; renal histopathological changes were observed by hematoxylin-eosin(HE) staining. Renal collagen deposition in rats was observed by Masson staining. [Results] The SCr in model group and the high, medium and low dose experimental groups were(340.00±22.99),(176.80±18.60),(234.75±13.59),(266.11±14.78) μmol/L, and BUN were(23.74±2.51),(14.53±2.25),(18.78±0.88),(18.90±2.14) mmol/L; 24 h MTP were(675.86±74.58),(323.81±41.83),(438.84±34.69),(493.76±37.04) mg/d; eGFR were(19.30±2.48),(49.96±10.95),(32.61±10.75),(27.18±5.98) mL/min, and the difference was statistically significant compared with the normal group(all P<0.05). HE staining and Masson staining showed that compared with normal group, the renal interstitial lesions in model group were severe and the renal interstitial collagen material was deposited in a large amount. The renal interstitial tubule injury was relieved and the renal interstitial collagen deposition was reduced in experimental groups. And the difference was statistically significant(all P<0.01). [Conclusions] Manshenkangning can significantly protect the kidney against the progress of interstitial fibrosis in rats. Its possible mechanism is to regulate the activity of SIRT1 and inhibit the expression of COX-2 in order to resist the inflammatory reaction of kidney and improve the ability of anti-oxidative stress of kidney, thus delaying the occurrence and development of chronic renal failure.

    2019年06期 v.10 35-38页 [查看摘要][在线阅读][下载 693K]
  • Review of Effects of Active Components of Gastrodiae Rhizoma on Central Nervous System

    Hua ZHU;Piaoling HUANG;Wenqi YANG;Anda WEI;Ruiting HE;Zhonghua DAI;

    In order to explore the pharmacological effects of active components of Gastrodiae Rhizoma on the central nervous system, through consulting related literatures, this paper summarized the pharmacological effects of the active components of Gastrodiae Rhizoma. The pharmacological effects of the active components of Gastrodiae Rhizoma on the central nervous system include improving the ability of learning and memory, analgesia, sedation and hypnosis, anticonvulsant, anti-aging, anti-anxiety and neuroprotective effects. The active components of Gastrodiae Rhizoma have certain curative effect in the treatment of some diseases of the central nervous system. At present, it has been widely used clinically. There are no toxic and side effects at conventional doses, and it is worth popularizing and applying.

    2019年06期 v.10 39-41页 [查看摘要][在线阅读][下载 127K]
  • Inhibitiont of Obazema on Proliferation of Gastric Cancer BGC-823 Cells and Its Mechanism

    Ying LI;Risha WEIZE;Hairong ZHONG;Jixiu SHEN;Yisong LI;Yuan LIU;

    [Objectives] This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism. [Methods] BGC-823 cells were treated with high, medium and low concentrations of drug-containing serum(0.316%, 0.158% and 0.079%) for 0, 48, 72 and 96 h, respectively. Then, the proliferation of the cells was detected with CCK-8 method, and the expression of related proteins, B lymphocytoma-2(Bcl-2), phosphorylated protein kinase B(p-Akt), protein kinase B(Akt) and glyceraldehyde-3-phosphate dehydrogenase(GAPDH), was detected using Western blotting. [Results] The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.) Reichb. ex Meisn. var. albiflora(CH) and B. sessilicarpa Lévl.(S)(P<0.01), in dose-dependent and time-dependent manners. The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration; the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01); different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells; the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05, P<0.01), in a certain dose-dependent manner; at the same dose, the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05, P<0.01); administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05, P<0.01). [Conclusions] Obazema has the capacity to inhibit the proliferation of BGC-823 cells. The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2, and GAPDH may be the target gene of its anti-tumor mechanism. The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.

    2019年06期 v.10 42-46+51页 [查看摘要][在线阅读][下载 1014K]
  • Study on the Hepatoprotective Effect of Oxalis coriniculata L. and Related Mechanism by Regulating Oxidative Stress and TLR-2/NF-κB Signaling Pathway

    Ya GAO;Chun CHEN;Kefeng ZHANG;Riming WEI;

    [Objectives] This study aimed to explore the protective effect of Oxalis coriniculata L. on rats with acute liver injury induced by carbon tetrachloride(CCl_4) and related mechanism by regulating oxidative stress and the TLR-2 TLR-2/NF-κB signaling pathway. [Methods] A total of 48 female rats were randomly and evenly divided into normal group, model group, silymarin group(0.12 g/kg), and high(16 g/kg), middle(8 g/kg) and low-dose(4 g/kg) O. coriniculata L. groups. The rats in the groups were intragastrically administered with 5 mL/kg of corresponding drugs(equal-volume distilled water for normal group and control group), respectively. The administration was conducted twice a day, for 10 consecutive days. After 2 h of the last administration, the rats in all the groups except the normal group were intraperitoneally injected with 12% carbon tetrachloride(CCl_4) olive oil solution(5 mL/kg), respectively to establish liver injury rat models. After 16 h, the eyeball blood of the rats was collected, and their liver tissues were collected for preparation of HE sections. The biochemical indicators detected included aspartate aminotransferase(AST), alanine aminotransferase(ALT), total superoxide dismutase(T-SOD) and glutathione peroxidase(GSH-Px) activity and malondialdehyde(MDA) content in the serum. The contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in the serum were detected by ELISA. The expression of Toll-like receptor-2(TLR-2) and nuclear factor-κB(NF-κB) in liver tissue was detected using Western blotting. The pathological changes of liver were observed under light microscope. [Results] Compared with the normal group, the ALT, AST activity and MDA, IL-1β, IL-6, TNF-α levels in rat serum significantly increased(P<0.01), the GSH-Px, T-SOD activity in rat serum significantly decreased(P<0.01), and the expression of TLR-2 and NF-κB in liver tissue was up-regulated(P<0.01) in the model group. Compared with the model group, the ALT, AST activity and MDA, IL-1β, IL-6 and TNF-α levels in rat serum reduced(P<0.05, P<0.01), the GSH-Px and T-SOD activity in rat serum increased(P<0.05, P<0.01), and the expression of TLR-2 and NF-κB in liver tissue was down-regulated(P<0.05, P<0.01) in the O. coriniculata L. administration groups. Pathological sections show that O. coriniculata L. had an improving effect on rats with acute liver injury induced by CCl_4. [Conclusions] O. coriniculata L. has a good protective effect on rats with acute liver injury induced by CCl_4. Its mechanism may be related to inhibition of oxidative stress, inhibition of inflammatory response and regulation of the TLR-2/NF-κB signaling pathway.

    2019年06期 v.10 47-51页 [查看摘要][在线阅读][下载 867K]
  • Antimicrobial Mechanism of Saponin from Sapindus mukorossi against Escherichia coli

    Faling LEI;Lei XIAO;Chunyan YAO;Zengli WANG;

    [Objectives] To investigate the antimicrobial activity and mechanism for saponin from Sapindus mukorossi against Escherichia coli. [Methods] E. coli was used as the test bacteria, and the antimicrobial effect of Sapindus saponin was studied by the minimum inhibitory concentration method. The antimicrobial mechanism of Sapindus saponin was studied in terms of growth curves, membrane potential, activity of cells, cell surface morphology and cell internal structure. [Results] The results indicate that Sapindus saponin can inhibit the growth of E. coli, the minimum inhibitory concentration is 5 mg/mL and has a significant inhibitory effect on the growth of E. coli. After treated with Sapindus saponin, the membrane potential of E. coli increased by 16.7, 17.3 and 19.1 times after exposition to 0.5, 1, 2 MIC Sapindus saponin respectively. PI staining results show that cell viability decreased and permeability of cell membranes increased. The results of SEM and TEM further corroborate the membrane damage, the cell was damaged after exposition to Sapindus saponin. [Conclusions] The antimicrobial mechanism of Sapindus saponin were to changed membrane potential of cell, and damage the cell membrane structures.

    2019年06期 v.10 52-55页 [查看摘要][在线阅读][下载 1394K]
  • Effects of Alcohol Extract of Giantleaf Ardisia Rhizome on Levels of Inflammatory Factors in Serum of Rats with Adjuvant Arthritis

    Weibo DAI;Fengrong WU;Pengpeng DONG;Congyan ZENG;Quanxi MEI;

    [Objectives] The purpose of this study was to investigate the effects of ethanol extract of Giantleaf Ardisia Rhizome on the serum levels of interleukin 6(IL-6), tumor necrosis factor α(TNF-α) and interleukin-1β(IL-1β) in adjuvant arthritis(AA) rats induced by Freund's complete adjuvant(CFA), so as to investigate its role in the treatment of rheumatoid arthritis. [Methods] Rat models of AA were induced by CFA, and they were given with different doses of ethanol extract of Giantleaf Ardisia Rhizome, followed by scoring of systemic and joint inflammation and swelling degree and determination of serum IL-6, TNF-α and IL-1β levels. [Results] Compared with the model group, the ethanol extract of Giantleaf Ardisia Rhizome significantly reduced the scores of systemic and joint inflammation and swelling degree and the serum levels of IL-6, TNF-α and IL-1β in AA rats(P<0.05, P<0.01). [Conclusions] The ethanol extract of Giantleaf Ardisia Rhizome can suppress the development of AA in rats and the mechanism may be related to the down-regulation of the expression of serum inflammatory factors(IL-6, TNF-α, IL-1β).

    2019年06期 v.10 56-59+65页 [查看摘要][在线阅读][下载 636K]

Processing of Traditional Chinese Medicine and Quality Control

  • Water Extraction Process of Chinese Herbal Compound Man Gan Ning

    Chunli TANG;Jiangcun WEI;Zhen XIE;Meiyan QIU;Jiabao MA;Fengxian ZHAO;Hongxia CHEN;

    [Objectives] To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination. [Methods] The L_9(3~4) orthogonal test was carried out, the comprehensive scores of ginsenoside Rb1 content, notoginsenoside R1 content, tanshinone I content and saikosaponin A content in the compound extract were used as evaluation indicators to explore the effects of water addition, the water addition, decoction time and decoction times on the water extraction process, and verification tests were carried out. [Results] The optimized water extraction process was as follows: adding 9 times of water in the first extraction, and extracting for 90 min; adding 7 times of water in the second extraction, and extracting for 60 min; adding 7 times of water in the third extraction, and extracting for 60 min. [Conclusions] The optimized water extraction process is stable and feasible, and can be used for the extraction of various herbs in Compound Man Gan Ning. It can also provide experimental basis for the formulation development of Compound Man Gan Ning sustained release tablet.

    2019年06期 v.10 60-65页 [查看摘要][在线阅读][下载 165K]
  • Determination of Adulteration Dye Auramine O in Gegen Qinlian Tablets by HPLC-MS/MS

    Xueyan ZHU;Liqing GU;Jiantong YUAN;

    [Objectives] This study aimed to establish a method for determination of adulteration dye auramine O in Gegen Qinlian tablets by HPLC-MS/MS. [Methods] The instrument conditions were as follows: column, Agilent Eclipse plus C_(18)(2.1 mm × 100 mm, 1.8 μm); mobile phase, acetonitrile-0.01 mol/L ammonium acetate; gradient elution; flow rate, 0.3 mL/min; column temperature, 35 ℃; injection volume, 2 μL; ion source, electrospray ionization source(ESI); and scanning mode, multiple reaction monitoring(MRM). [Results] Methodological validation shows that auramine O had a good linear relationship in the range of 0.093 4-1.869 pg(r=0.999 9), and the average recovery rate was 94.38%(RSD=3.60%, n=9). [Conclusions] The method established is accurate, convenient, precise, reproducible, and stable, with good linear relationship, and it can be used determine the content of adulteration dye auramine O in Gegen Qinlian tablets.

    2019年06期 v.10 66-67+71页 [查看摘要][在线阅读][下载 449K]
  • Establishment of Specific Chromatogram of Spica Prunellae Formula Granules Based on Standard Decoction

    Xinwei NONG;Mei YANG;Xiongmei HUANG;Huilian DENG;Chan WANG;Jian ZHOU;

    [Objectives] This study aimed to establish HPLC chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae. [Methods] The chromatographic conditions were as follows: column, SHISEIDO CAPCELL PAK C_(18) MGII column(4.6 mm×250 mm, 5 μm); mobile phase, acetonitrile-0.1% phosphoric acid; gradient elution; detection wavelength, 280 nm; flow rate, 1.0 mL/min; and column temperature, 30 ℃. The correlation between the decoction pieces, standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms. [Results] The fingerprint chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae showed five common peaks, with good correlation. Among the five common peaks, four of them were tanshinol, protocatechuic acid, caffeic acid and rosmarinic acid. [Conclusions] The main chemical constituents of decoction pieces, standard decoction and formula granules of Spica Prunellae are basically the same. The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.

    2019年06期 v.10 68-71页 [查看摘要][在线阅读][下载 891K]

Germplasm Resources and Cultivation

  • Establishment of Fingerprint of Cryptolepis buchananii Roem. et Schult. by HPLC

    Hongxia LI;Lu ZHANG;Mingcheng CHEN;Chenyan LIANG;Yunli TANG;Xiaoxun WANG;Ganneng HUANG;

    [Objectives] This study aimed to establish the fingerprint of Cryptolepis buchananii Roem. et Schult. by HPLC. [Methods] Taking the peak of chlorogenic acid as the references, the 10 batches of C. buchananii Roem. et Schult. materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5 μm, 4.6 mm×250 mm), detection wavelength of 323 nm, column temperature of 25 ℃, mobile phase of methanol-0.1% phosphoric acid solution, gradient elution and injection volume of 20 μL. The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A). [Results] Among the HPLC fingerprints of the 10 batches of C. buchananii Roem. et Schult. materials, there are 13 common peaks, and their similarity is all greater than 0.9, indicating good similarity. [Conclusions] This study can provide a scientific basis for the quality evaluation of C. buchananii Roem. et Schult.

    2019年06期 v.10 72-76页 [查看摘要][在线阅读][下载 544K]

Biotechnology

  • Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells

    Wangyuan ZENG;Wenxin DAI;

    [Objectives] To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells. [Methods] The in vitro cytology studies were carried out to confirm the expression of the AURKB gene in HCT 116 cells and make clear its role in cell activity, cell cycle control and apoptosis, and investigate the effect of AURKB gene in colorectal cancer(CRC). Quantitative reverse transcription/polymerase chain reaction(PCR) analysis and immunofluorescence(IF) staining for markers of AURKB gene were used to examine the effect of AURKB on HCT 116. The AURKB gene target was examined using western blot analysis. In addition, inhibition of AURKB expression was examined using RNA interference(RNAi) on HCT 116 cells in vitro. [Results] HCT 116 cells infected with AURKB shRNA virus suppressed expression of AURKB in vitro. AURKB gene knockdown HCT 116 cells showed reducing cell apoptosis in vitro. Finally, it demonstrated that AURKB function can induce apoptosis of HCT cells. [Conclusions] AURKB is a key regulator of colorectal cancer. AURKs are potential novel molecular targets for the prevention of cancer cell proliferation.

    2019年06期 v.10 77-86页 [查看摘要][在线阅读][下载 7307K]
  • Establishment of Variable-wavelength Fingerprint for Yixinshu Tablets

    Yuanchun QI;Yanchun ZHENG;Li CHU;Chunmin WANG;

    [Objectives] The purpose of this study was to establish the HPLC fingerprint of Yixinshu tablets, in order to provide a basis for quality control of Yixinshu tablets. [Methods] The chromatographic conditions were as follows: column, Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm, 5 μm); mobile phase, acetonitrile-0.02% phosphoric acid solution(gradient elution); column temperature, 25 ℃; detection wavelength, 280 nm(0-62 min) and 260 nm(62-125 min). Using salvianolic acid B as the reference, the fingerprints of nine batches of Yixinshu tablets were determined. The similarity between the fingerprints was evaluated according to the Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2012). [Results] A total of 25 common peaks were calibrated, 10 of which were identified. The similarity between the fingerprints of the nine batches of Yixinshu tablets and the reference fingerprint was all greater than 95%, indicating good similarity. [Conclusions] The established HPLC fingerprint can be applied to the quality control of Yixinshu tablets.

    2019年06期 v.10 87-89页 [查看摘要][在线阅读][下载 706K]

Clinical Application

  • Study on Quality Standards of Children Antipyretic Lotion

    Suhong QIN;Jiahuan WEI;Guangyuan XIE;Zhenyuan WEI;Lilong YANG;Yuqing ZHOU;Min HUANG;

    [Objectives] To establish suitable quality standards of children antipyretic lotion. [Methods] Qualitative identification was performed for Paederia scandens and Artemisia carvifolia in Children Antipyretic Lotion by thin-layer chromatography(TLC), and the content of paederosidic acid in children antipyretic lotion was determined by high performance liquid chromatography(HPLC). Besides, chromatographic column Feinigen XPeonyx C_(18)(250 mm×4.6 mm, 5 μm), mobile phase was used, gradient elution was carried out with acetonitrile-0.1% phosphoric acid aqueous solution, the detection wavelength was 236 nm, the flow rate was 1.0 mL/min, the column temperature was 30 ℃, and the injection volume was 10 μL. [Results] The thin-layer chromatography of P. scandens and A. carvifolia reference materials showed clear spots, good separation effect, strong specificity, and no interference in the limit control; paederosidic acid showed a good linear relationship in the range of 0.65-9.75 μg(R~2=0.999 6), the average sample recovery rate was 99.72%(RSD=2.52%). [Conclusions] The qualitative and quantitative methods established in this experiment are simple, reproducible, and the results are accurate and reliable, and can be applied as a quality control method for children antipyretic lotions.

    2019年06期 v.10 90-93页 [查看摘要][在线阅读][下载 873K]
  • Effects of Paeonol on Expression of Type Ⅰ and Type Ⅲ Collagen of High Glucose Induced Proliferation of Cardiac Fibroblasts

    Dandan WU;Xiaohui ZHOU;

    [Objectives] To explore the effects of paeonol on the inhibition of myocardial fibrosis in high glucose induced cardiac fibroblasts(CFs). [Methods] Differential adherence method was used to culture the primary CFs of neonatal rats(passage culture); CCK-8 method was used to detect the cell proliferation; MTT assay was used to screen the safe concentration of paeonol; the 2 nd to 3 rd generation CFs were randomly divided into normal group(5.5 mmol/L, expressed in C), high glucose group(30 mmol/L, expressed in HG), paeonol low dose group(Pae-L, 17.5 mg/L), and medium dose paeonol group(Pae-M, 35 mg/L), paeonol high dose group(Pae-H, 70 mg/L); Western Blot method was used to detect the expression of Col-I and Col-III protein. [Results] The extraction of CFs from primary neonatal rats was successful; high glucose(30 mmol/L) induction had a significant proliferation effect on CFs; compared with the normal group, the expressions of COI-I and Col-III protein were increased in the high glucose group(P<0.05); compared with the high glucose group, the expression of COI-I protein was decreased in each treatment group(P<0.05, P<0.01), and the decrease was most significant in the high dose group(P<0.01); the expression of COI-III in the high dose group was decreased and it was statistically significant(P<0.05). [Conclusions] Paeonol significantly inhibited the proliferation of high glucose induced CFs in neonatal rats. This experiment is intended to provide a new experimental basis for the prevention and treatment of diabetic cardiomyopathy(DCM).

    2019年06期 v.10 94-96+99页 [查看摘要][在线阅读][下载 658K]
  • Efficacy of Soya Lecithin Powder Combined with Conventional Therapy on Chronic Hepatitis B

    Weibin MO;Wan'ai OUYANG;

    [Objectives] To probe into the treatment effects of soya lecithin powder combined with conventional therapy on chronic hepatitis B. [Methods] A total of 120 patients with chronic hepatitis B admitted to the Eighth People's Hospital of Guangzhou from February 2017 to February 2018 were randomly divided into observation group and control group in the way of drawing lots, with 60 cases in each group. Both groups of patients were treated with conventional therapy. On this basis, the control group was given Liver Protection Tablet, and the observation group was given soya lecithin powder for 2 months. The relief of symptoms of both groups and the recovery of liver functions were observed, including alanine aminotransferase(ALT), aspartate aminotransferase(AST), serum total bilirubin(TBIL), and glutamyl transpeptidase(GGT), as well as recovery of B-scan ultrasonography image and adverse drug reactions were observed. [Results] After treatment, the relief rate of fatigue, anorexia, abdominal distension and the disappearance rate of hepatic discomfort in the observation group were significant higher than those in the control group, and the difference was statistically significant(P<0.05); the recovery rate of ALT, AST, TBIL and GGT levels in the observation group was higher than that in the control group, and the difference was statistically significant(P<0.05); the recovery rate of B-scan ultrasonography image in the observation group was significantly higher than that in the control group, and the difference was statistically significant(P<0.05). No significant adverse drug reactions were found in two groups during the drug administration. [Conclusions] Soya lecithin powder combined with conventional therapy on chronic hepatitis B can significantly improve the liver function and relieve the symptoms.

    2019年06期 v.10 97-99页 [查看摘要][在线阅读][下载 138K]
  • Effects of Ropivacaine-sufentanil Epidural Analgesia on Labor and Maternal and Neonatal Outcomes

    Tingyuan YAN;Junhuan WANG;Xuena CUI;Jin'e XU;

    [Objectives] This study aimed to investigate the effects of ropivacaine-sufentanil epidural analgesia on labor and maternal and neonatal outcomes. [Methods] A total of 180 primiparas in full-term pregnancy were selected. They were randomly divided into treatment group(n=90) and control group(n=90). The primiparas in the treatment group were injected epidurally with ropivacaine and sufentanil for analgesia, and the primiparas in the control group were subjected to vaginal delivery. The VAS scores at 5, 10, 30 and 60 min of analgesia were observed. The vaginal bleeding amount, total labor duration, neonatal Apgar score and vaginal delivery rate of the two groups were compared. [Results] Compared with the control group, the VAS score in the treatment group differed insignificantly after 5 min of analgesia(P>0.05), and decreased significantly after 10, 30 and 60 min of analgesia(P<0.05). The vaginal bleeding amount of the treatment group was significantly smaller than that of the control group(P<0.05). There was no significant difference in the neonatal Apgar score between the two groups(P>0.05). In the treatment group, the vaginal delivery rate increased(P<0.05), the second stage of labor was prolonged(P<0.05), and the first and third stages of labor did not change significantly(P>0.05). [Conclusions] Epidural analgesia with ropivacaine and sufentanil has a good analgesic effect and good safety, and is worthy of clinical promotion.

    2019年06期 v.10 100-101+104页 [查看摘要][在线阅读][下载 128K]
  • Application of Xiao’er Kechuanling Oral Liquid Combined with Tulobuterol Patch in Children with Bronchiolitis

    Zhen YAO;Haiping ZHANG;Fei WANG;

    [Objectives] To explore the use of Xiao'er Kechuanling Oral Liquid combined with Tulobuterol Patch on the basis of comprehensive treatment, compare it with the combined aerosol inhalation of terbutaline, ipratropium bromide and budesonide which are commonly used in clinic, and observe the curative effect in the treatment of acute bronchiolitis and the effect on serum IL-4 and IL-8. [Methods] 80 children with acute bronchiolitis were randomly divided into observation group(n=40) and control group(n=40), and both groups were given routine comprehensive treatment. The observation group was treated with Xiao'er Kechuanling Oral Liquid combined with Tulobuterol Patch and budesonide, while the control group was treated with combined aerosol inhalation of terbutaline, ipratropium bromide and budesonide. The curative effect of the two groups was observed; the remission duration of cough, wheezing, dyspnea and nocturnal wheezing, and the disappearance time of wheezing rales and moist rales were observed in the two groups; the treatment course of intravenous infusion of methylprednisolone sodium succinate, oxygen inhalation time, sputum suction times and hospitalization time were observed in the two groups; the changes of serum IL-4 and IL-8 were observed before and after treatment in the two groups; the adverse drug reactions were observed. [Results](i) The total effective rate of the two groups was 100%(P>0.05), but the cure rate of the observation group(72.5%) was significantly higher than that of the control group(42.5%)(P<0.01).(ii) The disappearance time of cough, wheezing rales and moist rales and hospitalization time in the observation group were significantly shorter than those in the control group(P<0.01), the remission duration of nocturnal wheezing in the observation group was shorter than that in the control group(P<0.05), and the remission duration of wheezing and dyspnea in the control group was slightly longer than that in the control group(P>0.05).(iii) There was no significant difference(P>0.05) in serum IL-4 and serum IL-8 between the two groups before and after treatment. After treatment, the levels of serum IL-4 and IL-8 in the observation group and the control group were significantly lower than those before treatment in the observation group and the control group. [Conclusions] On the basis of comprehensive treatment, the use of Xiao'er Kechuanling Oral Liquid combined with Tulobuterol Patch in the treatment of acute bronchiolitis could reduce the effect of nocturnal wheezing on sleep, reduce the levels of serum IL-4 and IL-8, and significantly improve adverse symptoms.

    2019年06期 v.10 102-104页 [查看摘要][在线阅读][下载 136K]

  • Medicinal Plant

    <正>Indexed by Chemical Abstracts(CA),AGRIS Database, CAB International(CABI),China National Knowledge Infrastructure(CNKI),Naresuan University Library, Indian Fund for Agricultural Development, Royal Tropical Institute, Library of Congress, ProQuest

    2019年06期 v.10 2页 [查看摘要][在线阅读][下载 634K]
  • AGRIS

    <正>AGRIS (The Agricultural Sciences and Technology) is a booklist-style international agricultural database which is established by the international agricultural technology information system subordinate to FAO. It embodies continued publications,documents,corpuses,

    2019年06期 v.10 65页 [查看摘要][在线阅读][下载 102K]
  • About KIT

    <正>The Royal Tropical Institute (KIT) in Amsterdam is an independent centre of knowledge and expertise in the areas of international and intercultural cooperation,operating at the interface between theory and practice and between policy and implementation. The Institute

    2019年06期 v.10 89页 [查看摘要][在线阅读][下载 277K]
  • Submit your manuscript today!MEDICINAL PLANT

    <正>Sponsored by the Wuchu(USA-CHINA) Science and Culture Media Corporation Medicinal Plant(ISSN 2152-3924) is an academic journal sponsored by USA-China Science & Culture Media Corporation in January 2010, published monthly in the general

    2019年06期 v.10 105页 [查看摘要][在线阅读][下载 483K]
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