Medicinal Plant

2010, v.1(08) 74-75+78

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Determination of Berberine Hydrochloride in Shihu Yeguang Pills by HPLC
Determination of Berberine Hydrochloride in Shihu Yeguang Pills by HPLC

摘要(Abstract):

[Objective] HPLC method was developed to determine the contents of berberine hydrochloride in Shihu Yeguang pills.[Method] C18 column(250 mm×4.6 mm,5 μm) was introduced in this study,acetonitrile-0.34% potassium dihydrogen phosphate-0.17% sodium dodecylsulphate(48:26:26) as the mobile phase,345 nm as the detection wavelength.[Result]The linear relationship of chlorogenic acid was good in the range of 0.042 8-1.154 8 μg,the recovery rate was 99.5% and the RSD was 2.8%.[Conclusion]The method is convenient and accurate that could be applied in quantitative determination of the medicine.

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