Medicinal Plant

2017, v.8(06) 40-42+47

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HPLC Finger-print Study of Marchantia convoluta in Guangxi
HPLC Finger-print Study of Marchantia convoluta in Guangxi

Yuefeng WANG;Xu FENG;Jian SU;Peng FU;Jipeng QIU;Hua ZHU;

摘要(Abstract):

[Objectives]To establish HPLC finger-print of Marchantia convolute Gao et Chang,and to control the quality of medicinal materials. [Methods] Agilent ZORBAX SB-C18 chromatographic column( 4. 6 mm × 250 mm,5μm) was adopted,acetonitrile( B)-0. 1% phosphoric solution( D) was used as the mobile phase( gradient elution),and the detection was conducted at the wavelength of 230 nm and at the column temperature of 25℃. HPLC finger-print of herb materials in the upland-field was established,and 8 lots of medicinal materials were used for the similarity assessment to establish the finger-print. [Results] Theprecision,repeatability and stability RSD of HPLC finger-prints of the 8 lots of Marchantia convoluta Gao et Chang were all less than 3%,and their similarities were all greater than 0. 75; 6 common peaks were observed,and the control finger-print was also established. [Conclusions] With reliable data and simple and efficient manipulations,HPLC finger-print analysis can provide references for the quality assessment of Marchantia convoluta Gao et Chang.

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Abstract:

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基金项目(Foundation): Supported by National Natural Science Foundation of China(NSFC)(No.81260620);; Zhuang Yao Medicines Collaborative Innovation Center[Gui JKY(2013)No.20];; Guangxi Zhuang Yao Medicines Key Laboratory[Gui KJZ(2014)No.32];; Guangxi Key Disciplines on Zhuang Medicine[Gui JKY(2013)No.16];; Studies of Bagui Scholars on Innovation Theory and Efficacy of TCM

作者(Author): Yuefeng WANG;Xu FENG;Jian SU;Peng FU;Jipeng QIU;Hua ZHU;

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