Medicinal Plant

2010, v.1(08) 95-97

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Determination of Coixol in Root,Testa and Stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf by HPLC
Determination of Coixol in Root,Testa and Stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf by HPLC

摘要(Abstract):

[Objective]To establish a method for the determination of coixol in root,stem and testa of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf herbs by HPLC.[Method]HPLC was used and the conditions were as follows:Agilent HC-C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid(V:V=25:75),flow rate of 1.0 ml/min,column temperature of 25 ℃ and detection wavelength of 232 nm.[Result] The contents of coixol in different parts of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.were as follows:root>testa>stem.[Conclusion]This method is accurate and specific,which is useful for the integrative exploitation of coixol in root,testa and stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.

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基金项目(Foundation): Supported by Key Research Projects of Natural Science of Department of Education of Guizhou Province(2008027)

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