Medicinal Plant

2014, v.5(02) 46-50

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HPLC Fingerprint of Pyrrosia calvata (Bak.) Ching
HPLC Fingerprint of Pyrrosia calvata (Bak.) Ching

Yan HUANG;Youmin WEN;Junhua MA;Buming LIU;Lu CHEN;Mingsheng CHEN;

摘要(Abstract):

[Objective]To establish the fingerprint of Pyrrosia calvata( Bak.) Ching for evaluating its quality by HPLC. [Methods] The HPLC analysis was carried out on SinoChrom ODS-BP( 5 μm,4. 6 mm × 250 mm) column with mobile phase of acetonitrile- 0. 2% phosphoric acid solution( gradient elution) at the flow of 1. 0 mL / min,and the detection wavelength was 205 nm. [Results]The established fingerprint of P. calvata was used to determine 10 batches of sample from different sources. 12 common peaks were confirmed as the character fingerprints through similarity analysis on the fingerprint of the P. calvata. [Conclusions]The method had good precision,stability and reproducibility, providing a scientific base for controlling the quality of P. calvata by establishing fingerprint of the P. calvata.

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基金项目(Foundation): Supported by the Key Scientific and Technological Project of Guangxi(1099062-4);; the Technology Innovation Project of Nanning(2009-2-15)

作者(Author): Yan HUANG;Youmin WEN;Junhua MA;Buming LIU;Lu CHEN;Mingsheng CHEN;

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