Medicinal Plant

2011, v.2(07) 63-66

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Study on the Separation and Purification of Total Saponins from RADIX ASTRAGALI by Macroporous Resin
Study on the Separation and Purification of Total Saponins from RADIX ASTRAGALI by Macroporous Resin

摘要(Abstract):

[Objective] The aim was to find out the optimum conditions for separation and purification of total saponins from RADIX ASTRAGALI.[Method] Employing ultraviolet spectrophotometry as dynamic adsorption-desorption method,the content of total saponins in RADIX ASTRAGALI was determined to reveal the optimum conditions for separating and purifying total saponins from RADIX ASTRAGALI with macroporous resin.[Result] The optimum conditions for separation and purification of total saponins with AB-8 were as follows:extract concentration of 0.1 mg/ml,sampling volume of 3 BV,flow rate of 1.0 ml/min,washing with 4 BV of distilled water first and then desorption with 4 BV of 50% ethanol.Under these conditions,the yield of total saponins was reached as high as 52.1%.[Conclusion] This method is simple and feasible,which could meet industrial requirements.

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基金项目(Foundation): Supported by Key Program from Education Department of Sichuan Province(2005A76)

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