Medicinal Plant

2010, v.1(11) 23-24+27

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Preliminary Study on Transformation of Endophytic Fungus XT5 in T. brevifolia by Taxadiene Synthase Gene
Preliminary Study on Transformation of Endophytic Fungus XT5 in T. brevifolia by Taxadiene Synthase Gene

摘要(Abstract):

[Object] To transform endophytic fungus XT5 of T.brevifolia by taxadiene synthase gene.[Method] The taxadiene synthase gene was transferred into T.brevifolia endophytic fungus XT5 by soil agrobacterium-mediated method,and the fermentation yield of taxol was studied.[Result] 8 recombinants were obtained,and the taxol productivity of strain XT5-TS-2 reached as high as 423.983 4 μg/L,53.19% higher than the original strain,which suggested the output of taxol can be improved effectively by taxadiene synthase gene.[Conclusion] The study would provide theoretical foundation for improving the fermentation yield of taxol by T.brevifolia endophytic fungus.

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基金项目(Foundation): Supported by Scientific Research Fund of Chongqing Municipal Education Commission (KJ061202,KJ081213)

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