Medicinal Plant

2010, v.1(07) 38-40+44

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Study on Extraction of Polyphenols from Punica granatum Linn.Peel and Its Antibacterial Activity
Study on Extraction of Polyphenols from Punica granatum Linn.Peel and Its Antibacterial Activity

摘要(Abstract):

[Objective]To optimize the extraction process of polyphenols from Punica granatum Linn.peel and discuss the antibacterial activity of the crude extracts.[Method]Microwave-assisted extraction(MAE) was used to extract polyphenols,and the extraction process was optimized by employing both the single-factor test and orthogonal test.The inhibitory activity of the crude extracts on Staphylococcus aureus,Proteus vulgaris and Bacillus cereus were compared.[Result]The optimum extraction conditions were as follows:screen of 60-80 mesh,extraction power of 385 W,solid-liquid ratio of 1:25(W/V,the same as following),extraction duration of 120 s and extraction times of 3,under which the yield of polyphenol from Punica granatum Linn.peel was 21.4%.The inhibitory activity of 0.96 mg/ml extracts on Staphylococcus aureus,Proteus vulgaris and Bacillus cereus were the strongest,the corresponding diameter of inhibition zone were 1.15,1.10 and 0.95 cm respectively and the corresponding MIC were 0.80,1.60 and 0.40 g/ml respectively.[Conclusion]The natural medicine was extracted from Punica granatum Linn.peel,which provided theoretical evidence for exploiting natural bacteriostat.

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基金项目(Foundation): Supported by Talents Training and Introducing Scientific research of Xihua University (R0820501)

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