Medicinal Plant

2018, v.9(06) 39-42

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Apoptotic Effect of Cryptotanshinone on Human Melanoma A375 Cells
Apoptotic Effect of Cryptotanshinone on Human Melanoma A375 Cells

Jinqian LI;Jiaru WANG;Lingqi MENG;Yi ZHANG;Hao WANG;Wanting XU;Yang LIU;Yu ZHANG;Shinong WANG;Yuqing ZHAI;Yinghua LUO;Chenghao JIN;

摘要(Abstract):

[Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on apoptosis of human melanoma A375 cells was detected by MTT colorimetry.The apoptosis of melanoma A375 cells was detected with Annexin V-FITC/PI and observed by fluorescence inverted microscope.The expression of apoptosis-related proteins was detected by Western blotting.[Results]The viability of the A375 cells decreased with the increase of drug concentration.The fluorescence intensity of the cells increased with the treatment time.The expression of pro-apoptotic protein caspase-3 gradually increased,while the expression of apoptosis-inhibiting proteins p-AKT and Bcl-2 gradually reduced.[Conclusions]Cryptotanshinone induces apoptosis of human melanoma A375 cells via AKT signaling pathway,thus exerting a good cytotoxic effect on A375 cells.

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基金项目(Foundation): Supported by Multigrain Production and Processing Characteristic Discipline Construction Project;; Postdoctoral Scientific Research Foundation of Heilongjiang Province of China(LBH-Q13132)

作者(Author): Jinqian LI;Jiaru WANG;Lingqi MENG;Yi ZHANG;Hao WANG;Wanting XU;Yang LIU;Yu ZHANG;Shinong WANG;Yuqing ZHAI;Yinghua LUO;Chenghao JIN;

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