Medicinal Plant

2012, v.3(08) 64-67

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

The Effect of Compound Danshen Dripping Pills on the Pharmacokinetics of Steady-state Warfarin in Rats
The Effect of Compound Danshen Dripping Pills on the Pharmacokinetics of Steady-state Warfarin in Rats

摘要(Abstract):

[Objective]To determine the potential interaction between the Chinese herb medicine,Compound Danshen Dripping Pills(CDDP)and a widely used oral anticoagulant warfarin in steady-state in vivo when administered concomitantly.[Method]Each day for 4 days the warfarin control group and the warfarin plus single-dose of CDDP group of rats were treated orally with warfarin(0.2 mg/kg,once per day at 8 a.m.),and the warfarin and CDDP combined treatment group of rats were treated orally with warfarin(0.2 mg/kg,once per day at 8 a.m.)and CDDP(500 mg/kg, once per day at 8 a.m.),the warfarin steady-state was achieved after 4 days of treatment,blood samples were collected each day(at 8 a.m.)for HPLC analysis.Until the 5 day the warfarin control group of rats were still treated orally with warfarin(0.2 mg/kg,once at 8 a.m.),the warfarin plus single-dose of CDDP group of rats were treated orally with warfarin(0.2 mg/kg,once at 8 a.m.)and CDDP(500 mg/kg,once at 8 a.m.), and the warfarin and CDDP combined treatment group of rats were still treated orally with warfarin(0.2 mg/kg,once at 8 a.m.)and CDDP(500 mg/kg,once at 8 a.m.),and blood samples were collected at 0,0.5,1,2,4,8,12,24,36,48 h after warfarin-treatment.The concentration of warfarin in blood plasma was determined by high performance liquid chromatography(HPLC).[Result]The linear calibration curve ranged from 0.05 to 10μg/ml,the limit of quantitation(LLOQ)for warfarin was 0.05 ng/ml(r>0.999),moreover,all the validation data including accuracy, precision(intra-and inter-day),sensitivity,recovery,stability,were within the required limits.CDDP did not alter the pharmacokinetic(PK)behavior of warfarin in steady-state in vivo in rats after simultaneous and concomitant treatment.[Conclusion]The simultaneous and concomitant treatment of rats with CDDP did not give rise to significant effects on the pharmacokinetics in steady-state of warfarin.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by National Key Special Project of Science and Technology for Innovation Drugs of China(Grant No.2008ZX09401-006)

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享