Medicinal Plant

2012, v.3(01) 52-53+57

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RP-HPLC Determination on the Content of Chlorogenic Acid in FLOS LONICERAE JAPONICAE and FLOS LONICERAE from Different Producing Areas
RP-HPLC Determination on the Content of Chlorogenic Acid in FLOS LONICERAE JAPONICAE and FLOS LONICERAE from Different Producing Areas

摘要(Abstract):

[Objective]To compare the differences in the content of chlorogenic acid between Shandong Pingyi,Henan Fengqiu FLOS LONICERAE JAPONICAE and Hunan Longhui,Chongqing Xiushan,Chongqing Wulong,Chongqing Jiangjin FLOS LONICERAE. [Method]The content of chlorogenic acid in FLOS LONICERAE JAPONICAE and FLOS LONICERAE from different producing areas was determined by RP-HPLC. The separation was performed on a chromatographic column Platisil ODS ( 250 mm ×4.6 mm,5 μm) with the detection wavelength at 326 nm. Following the injection of sample solution,an elution with acetonitrile-0.2% phosphoric acid as mobile phase ( V/V,17∶83) was initiated at a flow rate of 1.0 ml/min. [Result]The content of chlorogenic acid in Chongqing Xiushan,Chongqing Jiangjin,Chongqing Wulong and Hunan Longhui,FLOS LONICERAE was 9.03%,8. 62%,4. 51% and 3. 71% respectively,and that in Shandong Pingyi and Henan Fengqiu FLOS LONICERAE JAPONICAE was 4.08% and 3.79%. Of them,the content of chlorogenic acid was the highest in Chongqing Xiushan FLOS LONICERAE. [Conclusion]This method is reliable,stable and sensitive with high precision,strong resolving power and high recovery rate,and could be used for determining the content of chlorogenic acid in different producing areas of FLOS LONICERAE JAPONICAE and FLOS LONICERAE.

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