Medicinal Plant

2011, v.2(07) 35-38

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Study on the Extraction Technology of Chlorophyll from Leaves of Brassica juncea var.tumida Tsen et Lee by Pectinase-assisted Method
Study on the Extraction Technology of Chlorophyll from Leaves of Brassica juncea var.tumida Tsen et Lee by Pectinase-assisted Method

摘要(Abstract):

[Objective] To optimize the extraction technology of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method.[Method] Effects of enzymolysis temperature,ethanol volume fraction,extraction time and volume of extracting solvent on the extraction of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method were investigated through single factor test and quadratic regression rotation-orthogonal combination design.[Result] The optimum extraction conditions were as follows:enzymolysis temperature of 10 ℃,ethanol volume fraction of 40%,extraction time of 60 min,and volume of extracting solvent of 70 ml.The theoretical extraction rate of chlorophyll was 32.29 mg/g which was approximated to the experimental value of 31.87 mg/g.[Conclusion] This study could provide reference for rational development and utilization of Brassica juncea var.tumida Tsen et Lee and improvement of extraction rate of chlorophyll.

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基金项目(Foundation): Supported by the Science and Technology Project of Chongqing Education Commisson(KJ101316)

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