Medicinal Plant

2020, v.11(03) 34-38+42

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Optimization of Extraction Process and Antioxidant Activity of Polysaccharide in Embelia parviflora Wall. by RSM
Optimization of Extraction Process and Antioxidant Activity of Polysaccharide in Embelia parviflora Wall. by RSM

Wenli LI;Xianxian LIU;Xiaolian LIANG;Yong CHEN;Danqing PANG;Zhouyan HUANG;Qian HAN;Wenjun LIU;Liangni CHEN;

摘要(Abstract):

[Objectives] To optimize the extraction process of polysaccharide in Embelia parviflora Wall. by response surface methodology, and to study the antioxidant activity in vitro of polysaccharide in E. parviflora, so as to provide a basis for the further development and utilization of E. parviflora. [Methods] The solid-to-liquid ratio, extraction temperature, extraction time and extraction times were used as single factors to investigate the effects. On this basis, the response surface methodology(RSM) was used to optimize the extraction process of polysaccharide in E. parviflora. DPPH and ABTS free radicals were used to investigate the antioxidant capacity of polysaccharide in E. parviflora. [Results] The optimal solid-to-liquid ratio for extraction of polysaccharide from E. parviflora was 32∶1(mL/g), extraction temperature was 77 ℃, and extraction time was 36 min. The IC_(50) values of DPPH and ABTS were 0.04 and 0.03 mg/mL, respectively. [Conclusions] The optimized extraction process of polysaccharide in E. parviflora is stable, the extraction rate is high, and has strong antioxidant activity. It is expected to provide a reference for the industrial extraction of polysaccharide from E. parviflora.

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基金项目(Foundation): Supported by National Natural Science Foundation of China(81660701);; Natural Science Foundation of Guangxi(2016GXNSFAA380148);; Scientific Research Innovation Program of Guangxi University of Chinese Medicine(YJSZ201712);; Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6)

作者(Author): Wenli LI;Xianxian LIU;Xiaolian LIANG;Yong CHEN;Danqing PANG;Zhouyan HUANG;Qian HAN;Wenjun LIU;Liangni CHEN;

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