Medicinal Plant

2016, v.7(Z2) 37-41

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Optimization of SFE-CO_2 Extraction Process of Total Vol atile Oil from Citrisarcodactylisfructus by Box-Behnken Design-Response Surface Methodology
Optimization of SFE-CO_2 Extraction Process of Total Vol atile Oil from Citrisarcodactylisfructus by Box-Behnken Design-Response Surface Methodology

Siwei CHEN;Dan ZHANG;Zhuo ZHANG;Xin YU;

摘要(Abstract):

[Objective] To optimize the SFE-CO_2 extraction process of total volatile oil from Citrisarcodactylisfructus. [Methods] The influences of extraction temperature,extraction pressure,CO_2 flow,extraction time,acquisition pressure and acquisition temperature on the extraction rate of total volatile oil from Citrisarcodactylisfructus were investigated firstly. Moreover,four significant factors such as extraction temperature,extraction pressure,CO_2 flow and extraction time were selected to optimize the SFE-CO_2 extraction process by Box-Behnken Design-response surface methodology( BBD-RSM). [Results] The highest extraction rate of total volatile oil from Citrisarcodactylisfructus 1. 892% could be obtained when extraction pressure was 57 MPa; extraction temperature was 67 ℃; CO_2 flow was 22.0 L/h; extraction time was 4 h.It was verified by the further experiments and the relative standard deviation( RSD) between them was 2. 32%. [Conclusions] This experiment provides a reasonable method of optimizing the SFE-CO_2 extraction process of total volatile oil from Citrisarcodactylisfructus,which can promote the further development and utilization of volatile oil from Citrisarcodactylisfructus.

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基金项目(Foundation): Supported by the Scientific Research Project of Educational Commission of Sichuan Province,China(16ZB0205);; Funds for Distinguished Young Scholars of Sichuan Medical University(201208)

作者(Author): Siwei CHEN;Dan ZHANG;Zhuo ZHANG;Xin YU;

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