Medicinal Plant

2015, v.6(Z4) 34-37

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Properties of Phenylalnine Ammonialyase in Dioscorea opposita‘Tiegun' and Its Browning Control
Properties of Phenylalnine Ammonialyase in Dioscorea opposita‘Tiegun' and Its Browning Control

Xiting ZHAO;Liwei JIANG;Yannan LI;Lin MA;Huizhen WANG;Mingjun LI;

摘要(Abstract):

With Dioscorea opposita‘Tiegun'as the research materials,phenylalnine ammonialyase( PAL) characteristics related to browning and the effects of different inhibitors on PAL activity were studied by spectrophotometry. Research results showed that within the test range,the optimal p H was 8. 6 for PAL of D. opposita‘Tiegun'. PAL had poor tolerance to acid and alkali. The optimal temperature was 50 ℃,showing certain tolerance to high temperature. Even under incubation for 30 min at 80 ℃,there were still about 12% PAL activity. L-cysteine hydrochloride and citric acid had significant inhibitory effects on PAL; while phytic acid had no significant inhibitory effects on PAL activity; but phytic acid showed no significant inhibitory effects. Sodium chloride promoted the PAL activity. In order to effectively restrict the browning of fresh slices of D. opposita‘Tiegun'caused by PAL,processing and storage of D. opposita‘Tiegun'slices should be carried out under acidic condition( p H < 2. 0),low temperature( < 20 ℃) or high temperature( > 80 ℃). L-cysteine hydrochloride should be the first choice of antibrowning reagent without sulfite for fresh slices,followed with citric acid.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by the National Major Scientific and Technological Special Project for‘Significant New Drugs Development’(2009ZX09308-002);; the Key Scientific and Technological Project of Henan Province(112102210300);; the Natural Science Foundation of Education Department of Henan Province(2010A180015,2010B360002)

作者(Author): Xiting ZHAO;Liwei JIANG;Yannan LI;Lin MA;Huizhen WANG;Mingjun LI;

Email:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享