Medicinal Plant

2014, v.5(05) 18-21+26

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Study on the Effect of APS on the Proliferation and Cytokines Secretion of the Bone Mesenchymal Stem Cells Drived from Patient with Multiple Myeloma
Study on the Effect of APS on the Proliferation and Cytokines Secretion of the Bone Mesenchymal Stem Cells Drived from Patient with Multiple Myeloma

Hai BAI;Lin LIU;Cunbang WANG;Yaozhu PAN;Rui XI;Yuping WEI;

摘要(Abstract):

[Objectives] In this study,the influence of astragalus polysaccharide on proliferation and cytokine expression of bone mesenchymal stem cells( abbreviated as BMSC) of patients who were attacked by multiple myeloma( abbreviated as MM) would be analysed. [Methods]MM bone marrow mesenchymal stem cells(MM-BMSC) and normal human bone marrow mesenchymal stem cells(ND-BMSC) were isolated by the method of density gradient centrifugation. The 3rdgeneration BMSC was taken and measured growth curve and cytokine expression of cells. Respectively by MTT,flow cytometry,the influence of different concentrations of APS on cell proliferation and cell cycle of MM-BMSC. The method of ELISA was ultilized to meaure the expression levels of IL-1β and IL-6. [Results]MM-BMSC and ND-BMSC were successfully isolated and obtained. The cell growth curve showed MM-BMSC proliferated slowly. Its doubling time was of 86 h while the ND-BMSC doubling time was of 60 h,which comparatively,exsited statistically significant difference(P < 0. 05). Normally on the basis,levels of secreted IL-1β and IL-6 between MM-BMSC and ND-MSC exsited significant difference(P < 0. 05). The proliferation of MM-BMSC could be promoted by APS,in which the role of 1 mg /mL APS could reach the optimum(P < 0. 05). MM-BMSC basically kept in the state of stationary phase. If 1 mg /mL MM-BMSC disposed by APS was in the period of G0/G1,comparatively,the cell proportion was significantly lower(P <0. 05). While in the period of G2,the cell proportion was significantly higher(P <0.05). Furthermore,1 mg/mL APS could significantly reduce the levels of IL-1β and IL-6(P < 0. 05) secreted by MM-BMSC. [Conclusions]The levels of IL-1β and IL-6 secreted by bone marrow mesenchymal stem cells of multiple myeloma patients were in the state of abnormality. Meanwhile,1 mg /mL astragalus polysaccharides could promote the proliferation of bone marrow mesenchymal stem cells and lower its secretion of IL-1β,IL-6.

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基金项目(Foundation): Supported by Science and Technology Major Projects in Gansu Province (1102FKDA005)

作者(Author): Hai BAI;Lin LIU;Cunbang WANG;Yaozhu PAN;Rui XI;Yuping WEI;

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