Medicinal Plant

2012, v.3(02) 41-44

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Content Determination of Vitexin and Apigenin-7-O-β-D-Pyranglycuronide in Broussonetia papyrifera Leaves by HPLC
Content Determination of Vitexin and Apigenin-7-O-β-D-Pyranglycuronide in Broussonetia papyrifera Leaves by HPLC

摘要(Abstract):

[Objective] To determine the content of vitexin and apigenin-7-O-β-D-pyranglycuronide in Broussonetia papyrifera leaves.[Method] Using absolute methanol-1% acetic acid glacial(V/V,30:70) as mobile phase,the content of vitexin in B.papyrifera leaves was measured by HPLC method at the detection wavelength of 359 nm.Using absolute methanol-0.05% phosphoric acid(V/V,40:60) as mobile phase,the content of apigenin-7-O-β-D-pyranglycuronide was measured by HPLC method at the detection wavelength of 324 nm.[Result] There was good linearity in the concentration range of 0.056 to 0.488 μg/ml for vitexin(r=0.999 2).The average recovery rate of vitexin was 99.2% with the RSD of 1.42%.Apigenin-7-O-β-D-pyranglycuronide presented good linearity in the concentration range of 1.808 to 4.068 μg/ml(r=0.999 8).The average recovery rate of apigenin-7-O-β-D-pyranglycuronide was 100.9% with the RSD of 1.58%.[Conclusion] This method that takes advantages of simplicity and good separating effect could be used for establishing the quality standard of B.papyrifera leaves.

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基金项目(Foundation): Supported by the Science and Technology Special Program for Traditional Chinese Medicine of State Administration of Traditional Chinese Medicine (04-05ZP43)

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