Medicinal Plant

2010, v.1(06) 30-32

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Optimization and Purification of Expression Condition of Recombinant Tyrosol Glycosyltransferase
Optimization and Purification of Expression Condition of Recombinant Tyrosol Glycosyltransferase

摘要(Abstract):

[Objective]The aim was to study the preparation of salidroside basing on modern biotechnology. [Method] Basing on SDS-PAGE method, the expression condition of the recombinant gene was optimized by investigating the effects of induction temperature, concentration of IPTG and induction time on the gene expression. [Result]The optimum induction conditions were as follows: induction time of 5 h, IPTG concentration of 0.6 mmol/L and induction temperature of 37 ℃. The target protein with purity of 99% was obtained by purifying the fusion protein with Ni-chromatography column. [Conclusion]The study will provide successful experience for the purification of tyrosol glycosyltransferase.

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基金项目(Foundation): Supported by Natural Science Foundation of China (30900112);; Starting Foundation of Science Research of Jilin Agricultural University(205-00369)

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