Medicinal Plant

2013, v.4(10) 12-16

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Protective Effect of Panax notoginseng Saponins on Experimental Liver Fibrosis in Rats and to Explore Its Possible Mechanisms
Protective Effect of Panax notoginseng Saponins on Experimental Liver Fibrosis in Rats and to Explore Its Possible Mechanisms

Hui JIANG;Jiarong GAO;Jiafu ZHANG;Yan MA;


[Objective]This article is written to observe the protective effect of Panax notoginseng saponins on experimental liver fibrosis in rats and to explore its possible mechanisms.[Methods]SD rats were divided at random into normal group,model group,Panax notoginseng saponins group(50,100 and 200 mg/kg)and colchicines group(Col,0.1 mg/kg).Rats in each group,except those in the normal group,were given subcutaneous injections of 50%CCl4twice per week in consecutive 18 weeks in order to abduct the liver fibrosis in rat models.From the 9th model building week on,the medicine-given groups were severally given intragastric administration of the corresponding drugs for testing while the normal and model groups were given equal amount of menstrua,once per day for continuous 10 weeks.After the experiment,the contents of hyaluronic acid(HA),laminin(LN),procollagen III N-terminal peptide(PIIINP),and Collagen IV(CIV)were detected through radio-immunoassay and the level of malondialdehyde(MDA)and superoxide dismutase(SOD)in liver homogenate through colorimetric method.Meanwhile,liver tissues from fixed position were fetched and the degree of liver fibrosis was observed through HE and Masson staining.The expressions of Bcl-2 and Bax protein in liver tissues were detected through immunohistochemical method.[Results]Comparing to the model group,Panax notoginseng saponins(100 and 200 mg/kg)have an impact not only on relieving liver fibrosis degree in rats,reducing HA,LN,PIIINP,CIV and MDA contents,raising SOD level in liver tissues,but also on inhibiting Bax expression and promoting Bcl-2 expression.[Conclusions]Panax notoginseng saponins have a certain influence on anti-fibrosis of rats'livers by an up-regulation of Bcl-2 expression and a down-regulation of Bax expression.It can be assumed that the inhibition of liver apoptosis is one of its possible mechanisms.




基金项目(Foundation): Supported by Key Project of Anhui Provincial Natural Science Research in Colleges and Universities(No.KJ2001A187);; Clinical TCM Construction Projects of State Key TCM Subject[(2012)32]

作者(Author): Hui JIANG;Jiarong GAO;Jiafu ZHANG;Yan MA;