Medicinal Plant

2011, v.2(12) 56-59

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Study on the Extraction Processes of Polysaccharides from FOLIUM MORI
Study on the Extraction Processes of Polysaccharides from FOLIUM MORI

摘要(Abstract):

[Objective]To optimize the extraction process of polysaccharides from FOLIUM MORI. [Method]Three different methods like hot water extraction,water extraction-ethanol precipitation and ultrasonic-assisted extraction were adopted to extract polysaccharides from FOLIUM MORI. Taking the yield of polysaccharides as index,the extraction process of polysaccharides from FOLIUM MORI was optimized. [Result] In hot-water immersion method,the optimum condition for extracting polysaccharides was solid-liquid ratio of 1∶ 40 g /ml,extraction time of 1. 5 h and extraction temperature of 80 ℃,which yielded a polysaccharides yield of 11. 3%. In water extraction-ethanol precipitation method,the yield of polysaccharides was the highest at 9. 5% if sample was refluxed for 1. 5 h with 80% ethanol and then extracted for 1. 5 h at 80 ℃ in water bath. In ultrasonic-assisted method,the yield of polysaccharides was 11. 6% if FOLIUM MORI was treated for 10 min with 50 W ultrasonic power before the extraction for 1. 5 h at 80 ℃ with the solid-liquid ratio of 1∶ 40 g /ml. [Conclusion]Based upon the yield of polysaccharides,3 different extraction methods followed a trend of ultrasound-assisted extraction > hot water extraction > water extraction-ethanol precipitation. Considering cost,work efficiency,etc. ,ultrasonic-assisted extraction was showed to be the most promising method for extracting polysaccharides from FOLIUM MORI.

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