Medicinal Plant

2010, v.1(06) 36-38+42

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Study on the Anti-oxidation of Polyphenol from the Seeds of Toona sinensis (A. Juss) Roem in Vitro
Study on the Anti-oxidation of Polyphenol from the Seeds of Toona sinensis (A. Juss) Roem in Vitro

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[Objective] The research aimed to study the anti-oxidation of polyphenol from the seeds of Toona sinensis in vitro. [Method] The reducing power of total polyphenol extracts from the seeds of T. sinensis was measured by using reducing K3Fe(CN)6 method. The antioxidant activities of polyphenols from seeds of Toona sinensis (A. Juss) Roem were evaluated by ferric reducing antioxidant power assay, hydroxyl ( ·OH) free radical scavenging capacity assay, superoxide anion (O2·) free radical scavenging capacity assay,LO· and LOO·peroxidation reaction of PUFA of yolk LDL induced by Fe2+ scavenging capacity assay. [Result] The polyphenol from the seeds of T. sinensis had good reducing power and had obvious inhibition effects on · OH, O2-·, LO · and LOO ·, with certain dosage-effect correlation. The scavenging capacity of polyphenol from the seeds of T. sinensis on free radicals was weaker than ascohic acid, but its total reducing power was stronger ascorbic acid. [Conclusion] The polyphenol from seeds of T. sinensis in vitro had obvious anti-oxidation.

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