Medicinal Plant

2011, v.2(09) 62-64

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Extraction Processes of Total Sterol from LONGAN SEEDS
Extraction Processes of Total Sterol from LONGAN SEEDS

摘要(Abstract):

[Objective] To optimize the extraction process of total sterol from LONGAN SEEDS.[Method] Total sterol was extracted from LONGAN SEEDS by using reflux method and ultrasonic method.As for the reflux method,effects of reflux time,solid-liquid ratio and extraction times were studied by single factor test.As for the ultrasonic method,effects of ultrasonic time,solid-liquid ratio and extraction times were studied by L9(33) orthogonal test.Taking the extraction rate of total sterol as the index,total sterol content in LONGAN SEEDS using two different extraction processes was determined by UV-Vis spectrophotometer.[Result] The optimal extraction conditions by ultrasonic method were 1∶8 solid-liquid ratio,2 times of extraction and 20 min for each extraction.Under these conditions,the extraction rate of total sterol from LONGAN SEEDS was 4.662 mg/g.At the same time,the optimal extraction conditions by reflux method were 3 h extraction time,1∶8 solid-liquid ratio,and 3 times of extraction.Under these conditions,the extraction rate of total sterol from LONGAN SEEDS was 4.383 mg/g.[Conclusion] Ultrasonic method was selected to extract the total sterol from LONGAN SEEDS.

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