Medicinal Plant

2012, v.3(08) 27-30

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Optimization of PCR-DGGE Reaction System on Rhizosphere Soil Microorganism for Panax ginseng C.A.Mey
Optimization of PCR-DGGE Reaction System on Rhizosphere Soil Microorganism for Panax ginseng C.A.Mey

摘要(Abstract):

[Objective]To explore the PCR-DGGE reaction system on rhizosphere soil microorganism for Panax ginseng C.A.Mey.[Method] Some important factors for PCR-DGGE amplification system including the concentration of DNA template,Mg2+,dNTPs and primers were optimized with 1-year-old P.ginseng soil as materials.[Result]An optimal system(25μl)was obtained as 0.8μg/μl DNA template,0.2 mmol/L dNTPs, 0.4μmol/L primer,and 1.5 mmol/L Mg2+.[Conclusion]The method is simple and fast,which can lay basis for further analyzing the genetic diversity of rhizosphere soil microorganism for P.ginseng.

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