Medicinal Plant

2019, v.10(06) 35-38

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Effects of Manshenkangning Prescription on Adenine-induced Renal Interstitial Fibrosis in Rats
Effects of Manshenkangning Prescription on Adenine-induced Renal Interstitial Fibrosis in Rats

Linghui NIE;Aishan SU;Weifeng FENG;Long YI;Shiping ZHU;

摘要(Abstract):

[Objectives] To study the protective effects of Manshenkangning Prescription on adenine-induced renal interstitial fibrosis in rats, and explore the possible mechanism. [Methods] Sixty Wistar male rats were divided into normal group, model group, control group(administered with 10 mg/(kg·d) losartan) and high, medium and low dose experimental groups(30, 15, 7.5 mg/(kg·d) Manshenkangning). The rat models of renal interstitial fibrosis were induced by intragastric administration of adenine(250 mg/(kg·d)). After 2 h, the above drugs were administered intragastrically for 21 consecutive days and the administration time was 30 consecutive days. Serum creatinine(SCr), blood urea nitrogen(BUN), 24 h urinary protein(24 h MTP) and glomerular filtration rate(eGFR) were measured by biochemical method; renal histopathological changes were observed by hematoxylin-eosin(HE) staining. Renal collagen deposition in rats was observed by Masson staining. [Results] The SCr in model group and the high, medium and low dose experimental groups were(340.00±22.99),(176.80±18.60),(234.75±13.59),(266.11±14.78) μmol/L, and BUN were(23.74±2.51),(14.53±2.25),(18.78±0.88),(18.90±2.14) mmol/L; 24 h MTP were(675.86±74.58),(323.81±41.83),(438.84±34.69),(493.76±37.04) mg/d; eGFR were(19.30±2.48),(49.96±10.95),(32.61±10.75),(27.18±5.98) mL/min, and the difference was statistically significant compared with the normal group(all P<0.05). HE staining and Masson staining showed that compared with normal group, the renal interstitial lesions in model group were severe and the renal interstitial collagen material was deposited in a large amount. The renal interstitial tubule injury was relieved and the renal interstitial collagen deposition was reduced in experimental groups. And the difference was statistically significant(all P<0.01). [Conclusions] Manshenkangning can significantly protect the kidney against the progress of interstitial fibrosis in rats. Its possible mechanism is to regulate the activity of SIRT1 and inhibit the expression of COX-2 in order to resist the inflammatory reaction of kidney and improve the ability of anti-oxidative stress of kidney, thus delaying the occurrence and development of chronic renal failure.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by Supported by the Scientific Research Foundation Project of Traditional Chinese Medicine Bureau of Guangdong Province(20171075,20191093)

作者(Author): Linghui NIE;Aishan SU;Weifeng FENG;Long YI;Shiping ZHU;

Email:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享