Medicinal Plant

2010, v.1(08) 87-88+91

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Isolation and Determination of Catechin from Cynomorium songaricum Rupr
Isolation and Determination of Catechin from Cynomorium songaricum Rupr

摘要(Abstract):

[Objective] To establish the isolation and determination methods of catechin from Cynomorium songaricum Rupr.[Method] The dried Cynomorium songaricum Rupr.powder was extracted with 50% ethanol through ultrasonic and purified with macroporous absorption resin.HPLC conditions were as follows:C18 column(250 mm×4.6 mm,5 μm);mobile phase of acetonitrile-0.2acetic acid(15:85 V/V);room temperature as column temperature;flow rate of 1ml/min,detective wavelength of 280 nm.[Result] Catechin was concentrated in 35% ethanol elution fraction;the sample and the negative control were separated well,the linear range of catechin was 0.005-0.04 mg/ml(r = 0.999 9),the recovery rate was 98%,RSD was 0.658%.[Conclusions] Catechin can be isolated and purified from Cynomorium songaricum Rupr.effectively;HPLC is suitable for the determination with the advantages of small deviation and good reproducibility.

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基金项目(Foundation): Supported by the Project of Scientific and Technological planning of Beijing Education Authority(KM200811417002)

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