Medicinal Plant

2012, v.3(08) 25-26+30

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Determination of Flavonoids Content in Basella rubra L.Fruits by HPLC
Determination of Flavonoids Content in Basella rubra L.Fruits by HPLC

摘要(Abstract):

[Objective]To establish the determination method for flavonoids content in Basella rubra L.fruits.[Method]With rutin as the reference substance,chromatographic column was Eclipse Zorbax XDB-C 18 (4.6 mm×150 mm,5μm);mobile phase was anhydrous methanol-water (V/V,55∶45),flow rate was 0.8 ml/min;detection wavelength was 358 nm;and column temperature was room temperature.[Result]Under the chromatographic conditions,regression equation of rutin standard curve was Y=20.889X-17.147(R2=0.998 2).Linear regression was good between the concentration of 2.00 and 40.0μg/ml.The average recovery rate was greater than 96%and RSD was smaller than 2.0%.Flavonoids content in B.rubra fruits was 179.0μg/g by this method.[Conclusion]This method was simple,rapid,efficient,repeatable,accurate and reliable, and was suitable for the content determination of flavonoids in B.rubra fruits.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by the Scientific and Technological Innovation Project of Zhaoqing City(2011G210)

作者(Author):

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享