Medicinal Plant

2014, v.5(03) 34-36+40

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HPLC Fingerprint of Flavonoids from SEMEN TRIGONELLAE Extracts
HPLC Fingerprint of Flavonoids from SEMEN TRIGONELLAE Extracts

Jinqing LU;Qiang LI;Shengnan GUO;Junlong CAI;Xiaoshuang LI;Yi DAI;Huan LIANG;Xiaojin YANG;

摘要(Abstract):

[Objective]To establish the HPLC fingerprint of flavonoids from SEMEN TRIGONELLAE extracts.[Methods]ZORBAX SB-C18chromatographic column(4.6 mm×250 mm,5μm)was adopted;based on gradient elution,mobile phase was methanol-0.4%phosphoric acid solution.Flow rate was 0.8 mL/min;detection wavelength was 339 nm;and injection volume was 10μL.A total of 10 batches of SEMEN TRIGONELLAE extracts were measured by HPLC with vitexin as the reference substances.[Results]The common mode of the HPLC fingerprints was established;and a total of 11 common peaks were marked.The similarity degrees of the 10 batches of samples were all greater than0.9.[Conclusions]This method was simple,accurate and repeatable,and provided scientific basis for the quality control standard and quality evaluation of SEMEN TRIGONELLAE extracts.

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作者(Author): Jinqing LU;Qiang LI;Shengnan GUO;Junlong CAI;Xiaoshuang LI;Yi DAI;Huan LIANG;Xiaojin YANG;

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