Medicinal Plant

2019, v.10(06) 87-89

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Establishment of Variable-wavelength Fingerprint for Yixinshu Tablets
Establishment of Variable-wavelength Fingerprint for Yixinshu Tablets

Yuanchun QI;Yanchun ZHENG;Li CHU;Chunmin WANG;

摘要(Abstract):

[Objectives] The purpose of this study was to establish the HPLC fingerprint of Yixinshu tablets, in order to provide a basis for quality control of Yixinshu tablets. [Methods] The chromatographic conditions were as follows: column, Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm, 5 μm); mobile phase, acetonitrile-0.02% phosphoric acid solution(gradient elution); column temperature, 25 ℃; detection wavelength, 280 nm(0-62 min) and 260 nm(62-125 min). Using salvianolic acid B as the reference, the fingerprints of nine batches of Yixinshu tablets were determined. The similarity between the fingerprints was evaluated according to the Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2012). [Results] A total of 25 common peaks were calibrated, 10 of which were identified. The similarity between the fingerprints of the nine batches of Yixinshu tablets and the reference fingerprint was all greater than 95%, indicating good similarity. [Conclusions] The established HPLC fingerprint can be applied to the quality control of Yixinshu tablets.

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作者(Author): Yuanchun QI;Yanchun ZHENG;Li CHU;Chunmin WANG;

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