Medicinal Plant

2013, v.4(09) 29-31

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Determination of Araloside X in Aralia elata( Miq.) Seem. by HPLC
Determination of Araloside X in Aralia elata( Miq.) Seem. by HPLC

Yan LIU;Bing HE;Wei XIONG;Chunhong LI;Ji TIAN;

摘要(Abstract):

[Objective]To detect the Araloside X content in Aralia elata(Miq.)Seem.by HPLC.[Methods]Chromatographic column was Kromasil C18column(250 mm×4.6 mm,5μm);mobile phase was acetonitrile-0.05%phosphoric acid solution(35∶65,V/V);detection wavelength was 203 nm;column temperature was 30℃;flow rate was 0.8 mL/min;and injection volume was 10μL.[Results]Araloside X showed good linear relationship with peak area(r=0.999 9)within the range of 0.60-4.80μg/mL.The average recovery rate was 98.14%and RSD was 1.13%.[Conclusions]This method was sensitive,accurate and repeatable,and could be used for the quality control of A.elata.

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基金项目(Foundation): Supported by the Science and Technology Bureau of Luzhou City([2011]108)

作者(Author): Yan LIU;Bing HE;Wei XIONG;Chunhong LI;Ji TIAN;

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