Medicinal Plant

2010, v.1(10) 12-15

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Identification and Determination of Total Flavonoids from Prinsepia utilis Royle
Identification and Determination of Total Flavonoids from Prinsepia utilis Royle

摘要(Abstract):

[Objective] To establish the methods for the identification and determination of flavonoids components in Prinsepia utilis Royle.[Methods] Ethanol reflux extraction method was adopted to extract total flavonoids from Prinsepia utilis Royle,the physiochemical properties of which were identified by color reaction and thin-layer chromatography(TCL),the content of total flavonoids was determined by Al(NO3)-NaNO2 complexometry.[Results] The maximum absorption wavelength of sample solution was 510 nm and the solution was stable within 15 min.There was a good linear relationship between the concentration of rutin control solution(x) and absorbance value(y) with the linear regression equation of y=-2.571×10-3+13.163x,r=0.999 8;the average recovery rate was in the range of 98.00%-104.00%,101.16%was taken as average with RSD=2.15.It is detected that the flavonoids content in Prinsepia utilis Royle was 7.20%-7.30% with an average of 7.25%±0.05%,RSD=0.69%;the flavonoids content in oil meal of Prinsepia utilis Royle was 0.37%-0.38% with an average of 0.38%±0.01%,RSD=1.54%;The yield rate of flavonoids was 5.17%-5.25% with an average of 5.20%±0.04%,RSD=0.80%.[Conclusion] The method was simple,rapid,accurate and reliable,which could be applied to the determination of flavonoids in Prinsepia utilis Royle.

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基金项目(Foundation): Supported by Sichuan Provincial Special Fund of Animal Husbandry(2008B004)

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