Medicinal Plant

2012, v.3(10) 72-74

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Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L. Stems by Orthogonal Design
Optimization of Enzyme Extracting Process of Polysaccharides from Coix lachrymal-jobi L. Stems by Orthogonal Design

GUO Li-qiang;HUANG Li-de;ZHANG Zhao-ping;PAN Ting-qi;WEN Quan-tai;YAN Zu-di;HUANG Suo-yi;

摘要(Abstract):

[Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems. [Method] The cellulase extraction process of polysaccharides from C. lachrymal-jobi stems was optimized by means of orthogonal test design. And the content of polysaccharides in extracts was determined by spectrophotometry. [Result] The wavelength of the maximum absorption was 490nm. Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100mg/ml (R=0.9993). The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5, amount of enzyme was 6.0ml, the reaction temperature was 40℃, and enzymatic hydrolysis time was 150min. Under these conditions, the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%. [Conclusion] The enzyme extracting procedure is reasonable, economical and feasible.

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基金项目(Foundation): Supported by the Natural Science Foundation of Guangxi Province(2011GXNSFA018046);; the Science and Technology Project of Guangxi Traditional Chinese Medicine(GZKZ10-128);; the Project of Guangxi Education Department (201106LX425);; the Project of Guangxi Administration of Traditional Chinese Medicine(gzzc1047);; Open Special Fund of Experiment Center of Guangxi Medical Science(KFJJ2011-04);; the National Medical Association-funded Project of Youjiang Medical College for Nationalities(20110715)

作者(Author): GUO Li-qiang;HUANG Li-de;ZHANG Zhao-ping;PAN Ting-qi;WEN Quan-tai;YAN Zu-di;HUANG Suo-yi;

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