Medicinal Plant

2014, v.5(05) 13-17

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Research on the Technology of Ultrasonic Assisted Extraction of Flavonoids from the Stems and Leaves of Pratia begoniifolia
Research on the Technology of Ultrasonic Assisted Extraction of Flavonoids from the Stems and Leaves of Pratia begoniifolia

Lunpei LIU;Libo LIU;Xianhui SHAO;Fang LIAO;

摘要(Abstract):

[Objective] To study the optimal technology of ultrasonic assisted extraction of flavonoids from the stems and leaves of Pratia begoniifolia. [Methods]Ethanol with the concentration of 70% was taken as the reagent,and ultrasonic was used as an auxiliary extraction equipment. Main factors influencing extraction of flavnoids and the optimal level range of the factors were determined by single factor experiment,and the optimal extraction conditions were determined by L9(34) orthogonal test. [Results]Factors influencing extraction of flavnoids from the stems and leaves of Pratia begoniifolia Lindl. from primary to secondary were: ultrasonic power > leaching temperature > extraction duration >ratio of solid to liquid. The optimal conditions were: the ultrasonic power was 350 W,the leaching temperature was 60 ℃,the extraction duration was 30 min,and the ratio of solid to liquid was 1∶20(g /mL). Under these conditions,the content of flavonoids in stems and leaves of Pratia begoniifoli was 38. 437 mg /g. [Conclusions]The optimum conditions gained from the experiment can provide basis for the development and utilization of Pratia begoniifolia.

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基金项目(Foundation): Supported by Specialized Research Projects of Professors in Kaili University(No.JS201310);; Guizhou Province Key Support Disciplines:Botany Building Projects(No.Qianjiaogaofa(2011)275);; Basic Course Teaching Team Construction Project of Bio-science in Kaili University(JXTD201003)

作者(Author): Lunpei LIU;Libo LIU;Xianhui SHAO;Fang LIAO;

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