Medicinal Plant

2011, v.2(09) 20-21+24

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Determination of Bergenin in the Rhizoma Bergeniae Scopulosae by HPLC
Determination of Bergenin in the Rhizoma Bergeniae Scopulosae by HPLC

摘要(Abstract):

[Objective] To establish a method to determine the content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE by HPLC.[Method] The research was carried out on Kromasil-C18 column(4.6 mm×150 mm,5 μm) .And the mobile phase was acetonitrile-0.2% phosphoric acid(10∶90) with a flow rate of 1.0 ml/min.The column temperature was 35 ℃ and the detection wavelength was 275 nm.Thus,the content of bergenin in RHIZOMA BERGENIAE SCOPULOSAE was detected.[Result] Bergenin showed good linear relationship between the range of 0.248 and 1.240 μg/ml(r=0.997 9) ;and the average recovery was 100.95% with RSD=2.47%(n=6) .[Conclusion] This method was simple,repeatable and accurate and could be used for the quality control of RHIZOMA BERGENIAE SCOPULOSAE.

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基金项目(Foundation): Supported by the Foundation of Shaanxi Educational Committee(2010JM3014)

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