Medicinal Plant

2013, v.4(10) 27-29

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Effect of Polygonum Perfoliatum On the Expression of HBsAg and HBeAg in HepG2.2.15 Cells
Effect of Polygonum Perfoliatum On the Expression of HBsAg and HBeAg in HepG2.2.15 Cells

Ya GAO;Kefeng ZHANG;

摘要(Abstract):

[Objective]To study the effect of alcohol extraction of Polygonum Perfoliatum on the expression of HBsAg and HBeAg in HepG2.2.15cells.[Methods]The half toxic concentration(TC50)and the maximum non-toxic concentration(TC0)of Polygonum Perfoliatum extracts to HepG2.2.15 cells can be detected via methyl thiazolyl tetrazolium(MMT)method.With enzyme-linked immunosorbent assay(ELISA)method,the secretion level of HBsAg and HBeAg in cell supernates could be measured after a 72-hour action of drugs with four different concentrations on the HepG2.2.15 cells.[Results]Liquids with concentration of 0.4,0.2 and 0.1 mg/mL have an inhibitory effect on the secretion of HBsAg and HBeAg after being cultured for 72 h in the cell strain of HepG2.2.15 and there is a dose-response relationship between the concentration and the effect.Therapeutic indexes of the liquids were all greater than 1 but lesser than 2,which showed that alcohol extract of Polygonum Perfoliatum was an effective therapeutic drug of low toxicity.[Conclusions]Alcohol extraction of Polygonum Perfoliatum L.has an anti-HBV effect in vitro and certain toxicity as well.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): Ya GAO;Kefeng ZHANG;

Email:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享