Medicinal Plant

2010, v.1(07) 62-64+66

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Determination of Ursolic Acid and Oleanolic Acid Contents in Kuding Tea of Aquifoliaceae
Determination of Ursolic Acid and Oleanolic Acid Contents in Kuding Tea of Aquifoliaceae

摘要(Abstract):

[Objective] The research aimed at determining the contents of ursolic acid and oleanolic acid in different species and different origins of Kuding tea and providing basic data for the industrialization development of ursolic acid.[Method] The contents of ursolic acid and oleanolic acid in functional leaves from 6 species of Kuding tea in Aquifoliaceae and 4 kinds of the commercial Ilex kudingcha C.J.Tseng shoots were determined by high performance liquid chromatography(HPLC).The chromatographic conditions were as follows:Eclipse XDB-C18 column(5 μm,4.6 mm×150 mm),mobile phases of methanol-0.2% aqueous solution of phosphoric acid(v:v=88:12),detection wavelength of 220 nm,flow rate of 0.8 ml/min,column temperature of 35 ℃ and injection volume of 20 μl.[Result] The contents of ursolic acid from 6 species of Kuding tea in Aquifoliaceae were as follows:I.cornuta Lindl.(1.64%)>I.huoshanesis Y.H.He(1.63%)>I.latifolia Thunb.(1.54%)>I.pentagona S.K.Chen,Y.X.Feng et C.F.Liang(1.23%)>I.kudingcha C.J.Tseng(0.96%)>I.centrochinesis X.Y.Hu(0.69%)>the shoots of Ilex kudingcha C.J.Tseng(<0.28%).The contents of oleanolic acid from 6 species of Kuding tea in Aquifoliaceae were as follows:I.latifolia Thunb.(0.22%)>I.huoshanesis Y.H.He(0.18%)>I.cornuta Lindl.(0.17%)>I.pentagona S.K.Chen,Y.X.Feng et C.F.Liang(0.14%)>I.kudingcha C.J.Tseng(<0.12%)>I.centrochinesis X.Y.Hu(0.1%)>the shoots of Ilex kudingcha C.J.Tseng(0).[Conclusion] The determination results were favorable for the selection of suitable materials for the processing of ursolic acid.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by the Subproject of the National Key Technology R&D Program (2007BAD76B05-02)

作者(Author):

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享