Medicinal Plant

2011, v.2(06) 25-28+32

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Evaluation on the Developmental Toxicity of Peimisine by the System of Embryonic Stem Cells test
Evaluation on the Developmental Toxicity of Peimisine by the System of Embryonic Stem Cells test

摘要(Abstract):

[Objective] To investigate the effects of peimisine on developmental toxicity by the system of embryonic stem cells (ESCs) test. [Method] RT-PCR method was utilized to study the influence of peimisine at different concentrations of 0, 20, 40, 60 and 80 μg/ml on ESCs differentiating into cardiocytes with MHC specific expression after hanging drop and suspending culture and neuron cells with nestin specific expression after inducing by 5.0×10-7 mol/L retinoic acid. Effects of peimisine on cells viability of ESCs and BALBC(3T3) were assessed by alamar blue. Combined with the inhibitory effects on cells viability, the developmental toxicity characteristics of peimisine could be evaluated clearly. [Result] The inhibition concentration was the concentration of peimisine that could differentiate half ESCs into cardiocytes (ID50 D3 MHC) or neuron cells (ID50 D3 nestin) in vitro. The ID50 D3 MHC and ID50 D3 nestin of peimisine were 94.8 and 110.7 μg/ml, respectively. [Conclusion] Peimisine was identified as none-embryotoxicity and none-developmental neurotoxicity.

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基金项目(Foundation): Supported by Non-commercial and Central-class Special Research Foundation of Institutes (No.1381);; Support Project for the 11th Five-year Plan of Ministry of Science and Technology (2006BAI09B05-3);; Special Fund of Traditional Chinese Medicine Industry (200707007);; Key Foundation Program for Creating New Drugs of Ministry of Science and Technology (2009ZX09502-25);; 2008 Special Research Foundation of Traditional Chinese Medicine Industry (200807042)

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