Medicinal Plant

2011, v.2(12) 38-42

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Comparative Study on the Methods for Determining the Content of Flavonoids in G.biloba Tea and Leaf
Comparative Study on the Methods for Determining the Content of Flavonoids in G.biloba Tea and Leaf

摘要(Abstract):

[Objective]To compare the methods for determining the content of flavonoids in G. biloba tea and leaf that were cultivated in Qinba Mountain Area. [Method]The content of flavonoids in G. biloba tea and leaf was determined and analyzed by high performance liquid chromatography ( HPLC) ,ultraviolet spectrophotometry ( UV) and fluorescence spectrophotometry ( FL) . [Result] The content of flavonoids in 9 bathes of G. biloba ranged from 1. 15% to 3. 09% by using HPLC,while that ranged from 1. 22% to 4. 54% by using FL and fom 3. 47% to 10. 5% by using UV. As a result,the content of flavonoids determined by UV was three times higher than that determined by HPLC and that determined by FL,but there was no significant difference between HPLC and FL. The content of flavonoids in G. biloba tea produced from Qinba Mountain Area was determined by HPLC at 2. 65%. [Conclusion]The HPLC method is accurate in qualitative and quantitative analysis; the UV method is more easy and quick than other methods,which is suitable for the quality control of corporation.

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