Medicinal Plant

2010, v.1(10) 22-23+28

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Study on the Extraction Process of Total RNA from Dioscorea opposita Leaves
Study on the Extraction Process of Total RNA from Dioscorea opposita Leaves

摘要(Abstract):

[Object] To extract high quality total RNA from Dioscorea opposita leaves. [Methods] Leaves of Dioscorea opposite as tested materials, the guanidinium isothiocyanate method and modified CTAB method were introduced for extracting total RNA from Dioscorea opposita leaves, the integrity and purity of total RNA were analyzed by agarose gel electrophoresis and UV-spectrophotometer respectively. [Result] The 28S and 18 SrRNA bands of total RNA extracted by modified CTAB method were clear and visible, A260/A280 was in the range of 1.7-2.0. [Conclusion] Modified CTAB method was suitable for extracting total RNA from Dioscorea opposita Leaves.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by Scientific Research Foundation for Returned Scholars of Shaanxi University of Technology (SLGQD0715);; Special Scientific Research Foundation of Shaanxi Educational Department (08JK246)

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享